single molecule RNA FISH

Welcome single molecule RNA FISHer-people! This forum is intended to be a place where people can post

Hi all, I am considering to set up smFISH protocol in my lab. I need to estimate the costs of

Where do you think the adding of 0.5% Triton would help? after cells have been permeabilised? Thanks

Hyb longer than overnight should still be okay. I think we've even done 36 hours and it was the

So we just made a worked example for StarSearch (Mac): https://docs.google.com/document/d/

Hi there, I try to detect b-actin mRNA in MEFs by performing smFISH. we designed 36 probes with

Hello, I just started using the smFISH method on E.coli cells and have a few questions: * My main

Hey there, I am interested in using this method with DNA plasmids that are present in very low

So sorry for the late reply! The answer is yes, you can work with cells grown on coverslips. Just