ErrNo 32 during building markers database

[Violetta Florova]

 How can I solve this error?

*** ERROR ***  Cannot open 'tmp115201513281346060/clust/fams/tr|A0A024E4J3_9PSED_Alpha-subunit_of_nitrate_reductase_OS_Pseudomonas_mandelii_JR-1_GN_narG_PE_3_SV_1.faa' errno=22

Traceback (most recent call last):

  File "shortbred/shortbred_identify.py", line 275, in <module>

    pb.ClusterFams(dirClust, args.dClustID,strClustFile,args.dConsThresh,args.strMUSCLE )

  File "/cygdrive/e/Violetta/project1/shortbred/src/process_blast.py", line 339, in ClusterFams

    subprocess.check_call([strMUSCLE, "-in", str(fileFasta), "-out", str(fileAlign)])

  File "/usr/lib/python2.7/subprocess.py", line 186, in check_call

    raise CalledProcessError(retcode, cmd)

subprocess.CalledProcessError: Command '['muscle', '-in', 'tmp115201513281346060/clust/fams/tr|A0A024E4J3_9PSED_Alpha-subunit_of_nitrate_reductase_OS_Pseudomonas_mandelii_JR-1_GN_narG_PE_3_SV_1.faa', '-out', 'tmp115201513281346060/clust/fams/tr|A0A024E4J3_9PSED_Alpha-subunit_of_nitrate_reductase_OS_Pseudomonas_mandelii_JR-1_GN_narG_PE_3_SV_1.faa.aln']' returned non-zero exit status 2