Hi Nugraha,
<still looking for the best combination to analyze the sperm head
area.>
Here is an idea:
1: Run ShapeLogic Color Particle Analyzer with the mask option.
2: Run Process >> Binary >>Open (mathematical morphology), this should
remove the tail.
3: Run either the ShapeLogic Color Particle Analyzer again or the
ImageJ Particle Analyzer.
If this does not work you can send me an image and I will try to take
a look to see if I can come up with better idea.
-Sami Badawi