Exome- trio analysis

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Serena Spartano

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Oct 14, 2016, 9:40:20 AM10/14/16
to SeqMule-dev
Hi everyone I've got some problem while i try to perform a seqmule trio-exome analysis with the following command:

seqmule pipeline -a fa_R1.fq.gz,mo_R1.fq.gz,son_R1.fq.gz -b fa_R2.fq.gz,mo_R2.fq.gz,son_R2.fq.gz -ms -e -q -prefix father,mother,son -capture default

i didn't receive any error message but only a screen stuck into a synopsis of the pipeline's options.

Anybody here could help me please?

I attach below a printscreen of what i mean

Thank you very much in advance, I find seqmule a very useful tool for NGS

Best

Serena
Schermata seqmule del 2016-10-14 15:34:40.png

Yunfei Guo

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Oct 14, 2016, 12:07:43 PM10/14/16
to Serena Spartano, SeqMule-dev
Hi Serena,

'-t NUMBER_CPU' is required if you use quick mode (-q).

Also, what you saw is the detailed help of seqmule, which won't show up unless you specify '-h' or '--help' option. Could you please double check your command? Thanks.

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