Yes, you can make a screenshot of the entire screen, for example:
Private Function TakeScreenShot() As Bitmap
Dim screenSize As Size = New Size(My.Computer.Screen.Bounds.Width, My.Computer.Screen.Bounds.Height)
Dim screenGrab As New Bitmap(My.Computer.Screen.Bounds.Width, My.Computer.Screen.Bounds.Height)
Dim g As Graphics = Graphics.FromImage(screenGrab)
g.CopyFromScreen(New Point(0, 0), New Point(0, 0), screenSize)
Return screenGrab
End Function