China-Lyme Reverse Breakthru on Diagnosis

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Kathleen

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Nov 30, 2010, 6:52:30 PM11/30/10
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Subject: China-Lyme Reverse Breakthru on Diagnosis

Date: Nov 30, 2010 6:50 PM

China says each band is as accurate for diagnosis as its assign
percent specificity and that you only need one specific band. And if
you only have band 41 (flagellin), one should rule out syphilis or
leptospirosis - which was the original way Steere claimed we should
diagnose:
http://www.actionlyme.org/CRYMEDISEASE_CHP3_B.htm

Hurray!! They have sane people
and *real* *scientists* in China!
http://www.actionlyme.org/index.htm
=======================================

http://www.ncbi.nlm.nih.gov/pubmed/21112481

Biomed Environ Sci. 2010 Oct;23(5):341-9.
Interpretation criteria for standardized Western blot for the
predominant species of borrelia burgdorferi sensu lato in china.

Jiang Y, Hou XX, Geng Z, Hao Q, Wan KL.

State Key Laboratory for Infectious Diseases Prevention and Control,
National Institute of Communicable Disease Control & Prevention,
Chinese Center of Disease Control & Prevention, Beijing 102206, China.
Abstract

OBJECTIVE: Western blotting (WB; immunoblotting) is a widely used tool
for the serodiagnosis of Lyme borreliosis (LB), but so far, no
generally accepted criteria for its performance and interpretation
have been established in China. The present study was designed to
determine the criteria for standardized Western blot for the
predominant species of Borrelia burgdorferi sensu lato in China, in
which WB was produced with strain PD(91) as the representative strain
attributed to predominant genospecies Borrelia garinii of Borrelia
burgdorferi sensu lato.

METHODS: Approximately 13 bands between 14 and 100 kD were
differentiated for strain PD(91) by using Gel-Pro analysis software.
In a study with 631 serum samples (taken from 127 patients with Lyme
borreliosis and 504 controls), all observed bands were documented. To
establish criteria for a positive WB result for strain PD(91),
receiver operating characteristic (ROC) curves were used.

RESULTS: The following interpretation criteria were recommended: for
IgG, at least one band of P83/100, P58, P39, P30, OspC, P17, P66, and
OspA; for IgM, at least one band of P83/100, P58, OspA, P30, OspC, P17
or P41. In addition, syphilis, leptospirosis and other related
diseases should be excluded when the positive band is P41 in IgM. For
IgG criteria, the sensitivity is 73.2%, the specificity is 99.4% and
Youden index is 0.726; for IgM criteria, the sensitivity is 50.6%, the
specificity is 93.1% and Youden index is 0.437.

CONCLUSION: Standardization of WB assays is necessary for comparison
of results from different laboratories. Moreover, the criteria of
other genospecies of Borrelia burgdorferi sensu lato should be
determined in the future to complete the criteria of WB for the
diagnosis of the Lyme disease in China.
Copyright © 2010 The Editorial Board of Biomedical and Environmental
Sciences. Published by Elsevier B.V. All rights reserved.

KMDickson

Lipanj

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Dec 3, 2010, 10:38:09 PM12/3/10
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The Chinese are 100% CORRECT. It is also common sense..


On Nov 30, 6:52 pm, Kathleen <janmusin...@earthlink.net> wrote:
> To: margaret.hamb...@hhs.fda.gov, dwhe...@forbes.com,
> ca...@drcarolgoodheart.com, lPicker...@cdc.gov, Durland.f...@yale.edu,
> A...@columbia.edu, gary_worm...@nymc.edu,
> scientificintegr...@ostp.gov, pkrug...@princeton.edu,
> Stanley.f...@fiu.edu, margaret.hamb...@hhs.fds.gov,
> emcswee...@niaid.nih.gov, afa...@niaid.nih.gov,
> SpinL...@yahoogroups.com, kshep...@calea.org, fitz...@gmail.com,
> patrick.fitzger...@usdoj.gov, modelt1...@sbcglobal.net,
> jdra...@nejm.org, lett...@courant.com, Jgerberd...@cdc.gov,
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>
> Subject: China-Lyme Reverse Breakthru on Diagnosis
>
> Date: Nov 30, 2010 6:50 PM
>
> China says each band is as accurate for diagnosis as its assign
> percent specificity and that you only need one specific band. And if
> you only have band 41 (flagellin), one should rule out syphilis or
> leptospirosis - which was the original way Steere claimed we should
> diagnose:http://www.actionlyme.org/CRYMEDISEASE_CHP3_B.htm
>
> Hurray!!  They have sane people

> and *real* *scientists* in China!http://www.actionlyme.org/index.htm

Kathleen

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Dec 4, 2010, 5:53:28 PM12/4/10
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On Dec 3, 10:38 pm, Lipanj <lipanzmari...@aol.com> wrote:
> The Chinese are 100% CORRECT.  It is also common sense..

Yep.

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