ON Topic: Seronegative Lyme
Joanne822
Lyme? WRONG!
See the "Seronegativity" document at www.lymeinfo.net/lymefiles.html
for a summary of studies (including relevant quotes) showing that a person can
have Lyme disease despite negative serology tests.
Lisam01502
>Lyme? WRONG!
Someone close to me had two positive Elisas, plus all sx of Lyme, and stilll
they said she had no Lyme.
Lisa
Frank Andreas de Groot
news:20040109070950...@mb-m07.aol.com...
> Ever been told if you had a negative Elisa test, that you can't possibly have
> Lyme? WRONG!
I have a positive Immunofluorescent essay (IIRC) and a recent positive WB.
Both IgM and IgG.
When I went to the doctor, he said: "Get this test out of my eyes, I don't want
to know what the result is, we only trust tests that we do in our own hospital"
(in his hospital, Ullevål Sykehus in Oslo, Norway, Infectious disease
"specialist" Dr. Ormaasen does an ELISA, and trusts that more than 2 WB's by a
lab that tested for the 3 strains endemic to the country where I was infected.
Norway is supposedly the "best country to live in", according to the UN, but not
when it comes to Lyme. Norway wants to play important with the Peace Prize,
supporting human rights everywhere, except for their own Lyme people.
Chuck P Adams
>Date: 1/9/2004 10:29 AM Eastern
>Norway is supposedly the "best country to live in", according to the UN, but
>not
>when it comes to Lyme
I would not take a crap in Norway. I would rather live in Iran.
JWissmille
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These studies were found on both the Lymenet Search Server and OVID.
Unique Identifier: 89040109
Authors: Dattwyler RJ, Volkman DJ, Luft BJ, Halperin JJ, Thomas J and
Golightly MG.
Institution: Dept. of Med., State Univ. of New York, Sch. of Medicine,
Stony Brook 11794-8161.
Title: Seronegative Lyme disease. Dissociation of specific T- and
B-lymphocyte responses to Borrelia burgdorferi [see comments].
Source: New England Journal of Medicine. 319(22):1441-6, 1988 Dec 1.
Synopsis of Abstract:
3 groups studied.
(1) 17 patients with well documented clinically active chronic Lyme disease
who were seronegative on Western Blot, etc.
(2) 18 patients with clinically active chronic Lyme disease who were
seropositive on Western Blot, etc.
(3) Control group of healthy subjects (without Lyme disease).
The seronegative Lyme patients had a vigorous T-cell proliferative response
to whole B. burgdorferi, with a mean (+/- SEM) stimulation index of
17.8 +/- 3.3), similar to that (15.8 +/- 3.2) in the 18 seropositive
patients. T-cell response in both patient groups was greater than
the control group of healthy subjects (3.1 +/- 0.5; P < 0.001).
Conclusion: The presence of chronic Lyme disease cannot be excluded by
the absence of antibodies against B. burgdorferi and that a specific
T-cell blastogenic response to B. burdorferi is evidence of infection
in seronegative patients with clinical indications of chronic Lyme disease.
ISSN: 0028-4793
____________________
"...... This is the first study to report an association between PCR
positivity and the absence of a serologic response to Lyme borreliosis...."
Title: Polymerase chain reaction detection of Lyme disease: correlation with
clinical manifestations and serologic responses.
Authors: Mouritsen CL, Wittwer CT, Litwin CM, Yang L, Weis JJ, Martins TB,
Jaskowski TD, Hill HR
Source: Am J Clin Pathol 1996 May;105(5):647-54
Organization: Laboratory of Immunology and Infectious Diseases, Associated
Regional and University Pathologists, Salt Lake City, UT 84108, USA.
Abstract:
The authors have developed a simple, nested polymerase chain reaction (PCR)
assay for amplification of an outer surface protein A (OspA) gene fragment of
Borrelia burgdorferi using rapid temperature cycling and ethidium bromide
detection on agarose gels, and applied it to the diagnosis of Lyme disease in
humans. With denaturing and annealing temperature spikes instead of holds,
cycle times were less than 20 minutes for a 30-cycle amplification. Using
this rapid cycle PCR technique, as few as 5 spirochetes per mL of phosphate
buffered saline were detected. In addition, B burgdorferi DNA was detected
from spirochetes that had been spiked into one of several types of human body
fluids including serum, synovial fluid, and cerebrospinal fluid (CSF). A
number of clinical samples, which had been tested for Lyme immunoglobulin M
(IgM) and immunoglobulin G (IgG) antibody were also examined. In 29 serologic
positive samples (14 IgG and IgM positive, 9 IgM alone and 6 IgG alone), B
burgdorferi DNA was not detected. In contrast, nine serum samples and one
synovial fluid from patients with definite clinical features of Lyme disease
were found to be negative by EIA and Western blot analysis for IgG and IgM
antibody, but contained B burgdorferi DNA, as detected by PCR. Polymerase
chain reaction analysis of serum and synovial fluid may be of significant
diagnostic value in Lyme disease, especially in the absence of a serologic
response in early, partially treated and seronegative chronic disease. This
is the first study to report an association between PCR positivity and the
absence of a serologic response to Lyme borreliosis.
Language: Eng
Unique ID: 96202607
______________
TITLE: Seronegative chronic relapsing neuroborreliosis [see
comments]
AUTHORS: Lawrence C; Lipton RB; Lowy FD; Coyle PK
AUTHOR AFFILIATION: Department of Medicine, Albert Einstein College
of Medicine, New York, N.Y., USA.
SOURCE: Eur Neurol 1995;35(2):113-7
CITATION IDS: PMID: 7796837 UI: 95317331
COMMENT: Comment in: Eur Neurol 1996;36(6):394-5
ABSTRACT: We report an unusual patient with evidence of Borrelia
burgdorferi infection who experienced repeated neurologic relapses
despite aggressive antibiotic therapy. Each course of therapy was
associated with a Jarisch-Herxheimer-like reaction. Although the patient
never had detectable free antibodies to B. burgdorferi in serum or
spinal fluid, the CSF was positive on multiple occasions for complexed
anti-B. burgdorferi antibodies, B. burgdorferi nucleic acids and free
antigen.
_______________________________
Lyme disease and false negative or false positive blood test results
http://www.geocities.com/HotSprings/Spa/6772/false-neg-pos-index.html
This web site contains the following:
1. Introduction to Lyme disease and false negative/false positive blood
test results.
2. Summary and highlights of the selected abstracts on Lyme disease and
false negative/seronegative and false positive test results.
2a. Medical and scientific abstracts on Lyme disease and false
negative/seronegative test results.
2b. Medical and scientific abstracts on Lyme disease and false
negative/seronegative and false positive test results.
2c. Medical and scientific abstracts on Lyme disease and false positive
test results.
2d. Other medical and scientific citations (no abstract available on
internet) on Lyme disease and false negative/seronegative and/or
false positive test results.
3a. Doctors on Lyme disease and false negative/seronegative test
results.
3b. Doctors on Lyme disease and false negative/seronegative and false
positive test results.
3c. Doctors on Lyme disease and false positive test results.
4. Laboratories on Lyme disease and false negative/seronegative and
false positive test results.
5a. Other information on Lyme disease and false negative/seronegative
test results.
5b. Other information on Lyme disease and false negative/seronegative
and false positive test results.
5c. Other information on Lyme disease and false positive test results.
6a. Media articles about Lyme disease and false negative/seronegative
test results.
6b. Media articles about Lyme disease and false negative/seronegative
and false positive test results.
6c. Media articles about Lyme disease and false positive test results.
7. Lyme disease diagnostic testing resources.
8. Other Lyme disease resources.
also see:
Lyme Disease Resources
http://www.geocities.com/HotSprings/Spa/6772/resources.html
Lyme Disease in the United States and Canada
http://www.geocities.com/HotSprings/Spa/6772/lyme.html
If there are errors or something you'd like me to add,
please send me an email message.
Art Doherty
Lompoc, California
mailto:doh...@utech.net
___________
False negative Lyme disease blood test results due to use of antibiotics
************************************************************************
as of 28 October 1998
FDA Public Health Advisory:
Assays for Antibodies to Borrelia burgdorferi; Limitations,Use, and
Interpretation for Supporting a Clinical Diagnosis of Lyme Disease
July 7, 1997
"Several important factors contribute to the limitations of using ELISA,
IFA, or Western-blot tests for supporting Lyme disease diagnosis. The
stage of disease in which the specimen was taken is critical. First,
many patients with active or recent infections do not have detectable
anti-Bb in a single specimen. This happens because such antibodies often
develop subsequent to manifestations of early infection (the majority of
patients' sera are negative for IgM anti-Bb at presentation, whereas
most will have detectable IgM and/or IgG anti-Bb if sera are collected
and tested at both presentation and 2-4 weeks later) or because
detectable anti-Bb may diminish or never develop in patients treated
with antibacterial drugs."
FDA Public Health Advisory
http://www.fda.gov/cdrh/lyme.html
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Lyme Disease Diagnosis
Arthritis Foundation
Long Island Chapter
Lyme Disease Response Manual
What is meant by a false negative test?
A test which measures antibody levels (titers) is read as negative when
no antibodies are detected or if antibodies are found to be at a level
seen in people without disease. If the person does have Lyme disease,
this negative test result is called false negative. The use of
antibiotics early in the illness can retard the development of
antibodies.
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Arthritis Foundation - Lyme Disease Diagnosis
http://www.arthritis.org/offices/li/ldrm/diagnosis.shtml
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From Infections in Medicine®
Ticks and Lyme Disease
Author: Jerome Goddard, PhD, University of Mississippi School of
Medicine, Jackson, Miss.
Abstract:
.
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Lab analysis is complicated by the fact that persons who lack antibodies
to B burgdorferi during the early weeks of infection may not develop
antibodies following antibiotic therapy.[10]
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References:
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10.Magnarelli LA: Current status of laboratory diagnosis for Lyme
disease. Am J Med 98(suppl 4A):S10-S14, 1995.
.
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See complete article at:
Bug Vectors - Ticks and Lyme Disease
http://www.medscape.com/SCP/IIM/1997/v14.n09/m3040.goddard/m3040.goddard.html
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Title: Therapeutic Options for the Treatment of Lyme Disease
Authors: Ewa Kuna, MD, David J. Volkman, MD, PhD, State University of
New York at Stony Brook.
Abstract:
.
.
.
All 3 of the serological assays may give false-negative and false-
positive results. Most false-negative results (lack of sensitivity)
occur in early illness when antibodies are not yet present in serum. By
6 weeks after infection, more than 95% of untreated patients have
diagnostic levels of IgG anti-Borrelia antibodies. Even a small amount
of early antibiotic treatment aborts this development or persistence of
Borrelia-specific antibodies regardless of the efficacy of therapy in
eliminating the bacteria. A limited number of patients who had received
inadequate antibiotics early in their infection will progress to develop
later stages of Lyme disease but remain seronegative (false-
negative).[27]
.
.
.
References:
27.Dattwyler RJ, Volkman DJ, Luft BJ, et al: Seronegative Lyme disease:
Dissociation of specific T- and B- lymphocyte responses to Borrelia
burgdorferi. N Engl J Med 319:1441-1446, 1988. [See next below.]
.
.
.
See complete paper at:
Therapeutic Options for the Treatment of Lyme Disease
http://www.medscape.com/SCP/IIM/1993/v10.n06/m472.kuna/m472.kuna.html
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Title
Seronegative Lyme disease. Dissociation of specific T- and B-
lymphocyte responses to Borrelia burgdorferi [see comments]
Author
Dattwyler RJ; Volkman DJ; Luft BJ; Halperin JJ; Thomas J;
Golightly MG
Address
Department of Medicine, State University of New York, School of
Medicine, Stony Brook 11794-8161.
Source
N Engl J Med, 319(22):1441-6 1988 Dec 1
Abstract
The diagnosis of Lyme disease often depends on the measurement of
serum antibodies to Borrelia burgdorferi, the spirochete that causes
this disorder. Although prompt treatment with antibiotics may abrogate
the antibody response to the infection, symptoms persist in some
patients. We studied 17 patients who had presented with acute Lyme
disease and received prompt treatment with oral antibiotics, but in whom
chronic Lyme disease subsequently developed. Although these patients had
clinically active disease, none had diagnostic levels of antibodies to
B. burgdorferi on either a standard enzyme-linked immunosorbent assay or
immunofluorescence assay. On Western blot analysis, the level of
immunoglobulin reactivity against B. burgdorferi in serum from these
patients was no greater than that in serum from normal controls. The
patients had a vigorous T-cell proliferative response to whole B.
burgdorferi, with a mean ( +/- SEM) stimulation index of 17.8 +/- 3.3,
similar to that (15.8 +/- 3.2) in 18 patients with chronic Lyme disease
who had detectable antibodies. The T-cell response of both groups was
greater than that of a control group of healthy subjects (3.1 +/- 0.5; P
less than 0.001). We conclude that the presence of chronic Lyme disease
cannot be excluded by the absence of antibodies against B. burgdorferi
and that a specific T-cell blastogenic response to B. burgdorferi is
evidence of infection in seronegative patients with clinical indications
of chronic Lyme disease.
Language
Eng
Unique Identifier
89040109
http://www.infotrieve.com/healthworld/std_format.cgi?med85-89+1283743+((ly
me+OR+burgdorferi+OR+borreliosis)+AND+seronegativ*+AND+NOT+'false+negative')