PQQ and mt biogenesis
jw
J Biol Chem. 2009 Oct 27. [Epub ahead of print]
Pyrroloquinoline quinone stimulates mitochondrial biogenesis through
CREB phosphorylation and increased PGC-1{alpha} expression.
Chowanadisai W, Bauerly KA, Tchaparian E, Wong A, Cortopassi GA,
Rucker RB.
UC Davis, United States;
Bioactive compounds reported to stimulate mitochondrial biogenesis are
linked to many health benefits, such increased longevity, improved
energy utilization, and protection from reactive oxygen species.
Previously studies have shown that mice and rats fed diets lacking in
pyrroloquinoline quinone (PQQ) have reduced mitochondrial content.
Therefore, we hypothesized that PQQ can induce mitochondrial
biogenesis in mouse hepatocytes. Exposure of mouse Hepa1-6 cells to
10-30 mM PQQ for 24-48 h resulted in increased citrate synthase and
cytochrome c oxidase activity, Mitotracker staining, mitochondrial DNA
content, and cellular oxygen respiration. The induction of this
process occurred through the activation of cAMP response element
binding protein (CREB) and PPAR-gamma-coactivator-1alpha (PGC-1alpha),
a pathway known to regulate mitochondrial biogenesis. PQQ exposure
stimulated phosphorylation of CREB at serine 133, activated the
promoter of PGC-1alpha, and increased PGC-1alpha mRNA and protein
expression. PQQ did not stimulate mitochondrial biogenesis following
siRNA-mediated reduction in either PGC-1alpha or CREB expression.
Consistent with activation of the PGC-1alpha pathway, PQQ increased
nuclear respiratory factor activation (NRF-1 and NRF-2) and Tfam,
TFB1M, and TFB2M mRNA expression. Moreover, PQQ protected cells from
mitochondrial inhibition by rotenone, 3-nitropropionic acid, antimycin
A, and sodium azide. The ability of PQQ to stimulate mitochondrial
biogenesis accounts in part for action of this compound and suggests
that PQQ may be beneficial in diseases associated with mitochondrial
dysfunction.
PMID: 19861415 [PubMed - as supplied by publisher]
trigonometry1972@gmail.com |
PQQ in Natural Products
Unit: mug/kg or mug/L
Apple 6
Kiwi Fruit 27
Orange 7
Papaya.............27
Tomato 9
Cabbage 16
Carrot ........... 17
Celery 6
Green Pepper 28
Parsley 34
Green Tea..........30
Broad Beans 18
Soybeans 9
Cocoa Powder......800
Natto ..............61
Human Milk 140 to 180
Cow milk 3.4
Egg Yolk 7
Egg White...........4.1
From: ADVANCES in orthomolecular
research 23-27
---------------------
My comment: I found this on a chemical sales
site. For some reason it didn't cut and
paste. I did try to 'reflect' the entry
when I typed it though the format is different.
I wonder what sort of PQQ one might
find in nutritional yeasts? Or in
'fermented' cheeses?
jw
disguise for a PQQ supplement ; ) This nicely complements the
research on cocoa benefits.
I tried to access the "free" article and discovered that it isn't
free.
Paul Antonik Wakfer
I have access to the full text, but this journal does not appear to
have pdf's for issues since 1995.
--Paul Wakfer
MoreLife for the rational - http://morelife.org
Reality based tools for more life in quantity and quality
The Self-Sovereign Individual Project - http://selfsip.org
Self-sovereignty, rational pursuit of optimal lifetime happiness,
individual responsibility, social preferencing & social contracting
trigonometry1972@gmail.com |
> On Dec 27, 2:09 am, jw <jwesl...@gmail.com> wrote:
>
> > Excellent. Thanks for posting that. It looks like cocoa is a clever
> > disguise for a PQQ supplement ; ) This nicely complements the
> > research on cocoa benefits.
> > I tried to access the "free" article and discovered that it isn't
> > free.
>
> I have access to the full text, but this journal does not appear to
> have pdf's for issues since 1995.
>
> --Paul Wakfer
>
> Reality based tools for more life in quantity and quality
> Self-sovereignty, rational pursuit of optimal lifetime happiness,
> individual responsibility, social preferencing & social contracting
JW and Paul
Here is a link that if one clicks the pdf link one get a paper
with a similar food analysis. It seems more complete.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1136652/
This analysis is in ng/g or ng/mL.
Clearly the doses we get from foods is pretty small
compared to some of the other dietary quinones such
vitamin E forms, phylloquinone, and menaquinone.
What do this paper mean by "Coke"? 20 ng/g
I just got started so I may find some other things..........Trig
trigonometry1972@gmail.com |
Of course, the other dietary quinones look pretty different
structurally
and are fat soluble; whereas, PQQ is water soluble and chemical
stable. So the association of activity of these other chemicals isn't
relevant, the best I can see. Sorry.
Paul Antonik Wakfer
> On Dec 27, 2:09 am, jw <jwesl...@gmail.com> wrote:
>
> > Excellent. Thanks for posting that. It looks like cocoa is a clever
> > disguise for a PQQ supplement ; ) This nicely complements the
> > research on cocoa benefits.
> > I tried to access the "free" article and discovered that it isn't
> > free.
>
> I have access to the full text, but this journal does not appear to
> have pdf's for issues since 1995.
To be clearer, it is jw' originally posted study, PMID: 19861415, to
which I refer above.
-Paul Wakfer
MoreLife for the rational -http://morelife.org
Reality based tools for more life in quantity and quality
The Self-Sovereign Individual Project -http://selfsip.org