Longevity technology

[Treon Verdery]

GSK

Peelies from sun exposure relief technology: 10hda is published as reducing scratching multiple times; screen a decanoic ester library, that finds a molecule that does better than the multiple times less scratching that nude mice do when they have dermatitis and have royal jelly topically applied to them while being at greater potency per mg or microgram, Put some kind of dermis passing thing on or with it, beauty emulsions say they penetrate the dermis to bring active ingredients to the dermis so those technologies can bring decanoic acid esters to the living part of the dermis

so it can soak through the peelie and get to the itchy live dermis under the peelie. I have also read multiple pubmed journal references comparing topical dermis youthifiers and the references say some of them are highly functional so dermal transport media or moieties are to my perception functional

Oral version

Topically, harmless antibodies linked to fluoroethynyl phenibut like chemical, could possibly concentrate at the dermis.

An oral version with enteric coated liposomes to deliver the protein conjugate drug could take numerous minutes to reach the small intestine but make people feel better for many hours and as a pill is more likely, I perceive, to be utilized.

The possibility of a fluoroethynyl peptide or a harmless antibody linked peptide suggests less than 100 micrograms would dose the entire volume of a 70kg body, so a few kg of dermis is likely less than 11 micrograms per application, at $435/g (retail antibodies) that is near 43.5 cents a milligram or near .0435 cents per topical dose or .29 of a cent per oral dose

Is there such a thing as well feeling and appearing dermis, itchless and zero hue change skin puffiness causing chemical; one possibility that is similar but kind of different is topical vasodilator, possibly niacin, which i perceive i may have read about as a topical beauty treatment; if there is then doubling fluid flow at the capillary epithelia could make a topical peelie comfort medicine absorb twice as rapidly or ultra hydrate the dermis causing more rapid diffusion and greater activity of the topical drug causing more rapid function; I have heard of vasoactive peptides, perhaps they could function;

A zero hue change completely comfortable dermal vasodilator might also double the effectiveness of things like peptide beauty topicals, it makes me think though, they could just double the amount of active ingredient;

Find out if topical or oral phenibut, possibly a halogenated phenibut, or GABA active peptide effects dermal nerves to cause the removal of itching that about 2 grams of oral phenibut causes. A body side of the blood brain barrier possibly halogenated phenibut or other body side of the blood brain barrier GABA active molecule could omit CNS GABA activities, it is beneficial to have the drug be absent effects on things like driving ability, reaction time, or social skills.

Thick throat coating drink with peptides might have sufficient surface area to be absorbed as a kind of oral-like peptide dosing form, although I have heard of peptide doses up to 1 mg my perception is that at things like vasopressin and oxytocin it is much less, the most dose effective peptide I have read about, having not looked up other things is a mu opioid peptide active at nanogram amounts, I perceive I read about a halogenated peptide that was twice as effective; at an oral like mouth and throat coating peptide dosing form it is possible the pH of throat saliva could be modified and a mucolytic thing, perhaps something like a protease that is absent an effect on the peptide could make the peptide actually contact the dermis at the throat and mouth, the thing is though, what about stirring? A mm layer of colorant will just sit at a glass, for I perceive, hours, before diffusing; effervescence could do it, so perhaps a bubbly mini-beverage could make the body surface dermis soothing peptide reach the throat mucosa dermis many times more rapidly and before it was washed to the stomach; beneficially the peptide would only work at the mouth and throat so if many doses were consumed because the bubbly mini-beverage tastes good the dose would not vary that much

The 1/10 of a cent printed paper dose technology with the 1 cm^2 doses and enteric layered ink could be beneficial at the developing world, the thing is, what drug, like antibody linked GABA active peptide or drug, would soothe the dermis; tetracycline and possibly minocycline do build up at the dermis, possibly a GABA active drug linked to one of those molecules that has been screened to be a neutral biotic, possibly also finding a variant with greater dermal localization

An all natural product could also function, I do not know of any anaesthetic macroscopic or other fungi, but if there are any, notably GABA receptor active fungi chemicals, then those could be a rapid product.

Actually I am not aware of any natural product that effects GABA receptors, there are commercial natural products chemical libraries that exist, they could screen those to find out if any of the natural products effect GABA receptors, GABA the chemical does not pass the blood brain barrier but might diffuse through the dermis and is only a few $ per 100g.

Other neurotransmitters at the dermis could be anesthetizing, heightening response of anadamide receptors is unknown, a mu-opiate peptide that disintegrates in saliva, mucous of any orifice, and the stomach could be a topical anesthetic, perhaps a mu opiate peptide with a magnesium or calcium or zirconium or scandium atom in the middle of it could persist long enough at the dermis, or lasting about 90 seconds, but continually diffusing from the topical material would function

Orally a mu opioiod peptide attached to a harmless dermal

Localizer molecule, possibly an antibody or a minocycline variant could function. As a topical drug the peptide could be modified so that many enzymes degrade it so it would be nonseperable from the localization chemical which might also be made of enzymatically degradable nucleotides, peptides or proteins

Things that soothe UV Elie's could ask soothe dermatitis as well as a topic dermatitis

Theoretically about7.6 billion people on earth have experienced sun based peelies, so this could benefit people

I have not read about brain area localization technologies applied to mental illness drugs but it seems possible it happens, area (like neocortex) is a different thing than neurochemistry, like say all the neurons with AMPA receptors, or at antischizophrenia medication 5HT2 receptors at pimavanserin,

They could screen a library of fungal chemicals, noting fungi have numerous unusual chemicals after utilizing a radiolabelled acetylating chemical to acetylate a bulk fungal extract, so the chemicals pass the blood brain barrier they could separate the chemicals (more on that at a different note) and feed them to mice, one new chemical each 24 hours, and then brain scan them, and with 300 mice a month later would have 9000 localization maps; if 1 out of 100 had utility they would have 90 maps, also they get maps of where things localize at the body area as well; then noting what the concentration areas are they attach drugs to that fungal molecule and see if the molecules still go to the mapped area; they could come up with molecular variations on the fungal molecules that were absent any drug effect so the drug molecules the fungal molecules were transporting did all the pharmacological activity, it is possible that enzymatically degradable linker molecules could separate the active drug from the fungal transporter. They might do things like this now I do not know.







Separating chemicals from each other with microfluidics,

Another possible way is ion exchange resin inkjet printed beads, i think i have heard of printed microfluicics and i have heard of 3D printed microfluidics, it might be possible to inkjet print digital gradients of ion exchange resin component chemicals, then aim a warming laser to melt them so they bead up, then high frequency nonwarming pulse interval laser to stir them, making a digital gradient of different ion exchange resins responsive to a different chemical; a microfluidic channel layer is then printed atop this (or previously beneath) and each of the separate ion exchange chemical concentrators has its own plumbing, at 32 micrometers radius of the bead then there are 47,851 chemically different chemical separation locations on a 7 cm times 7 cm chip

and possibly

or if 3D printing are printed on peltier cooled paper; or 72°F stable then they melt, liberating contents when a laser shines on them

Another way to make an IER chip is ion exchange resin inkjet printed beads, i think i have heard of printed microfluidics and i have heard of 3D printed microfluidics. It might be possible to inkjet print digital gradients of ion exchange resin component chemicals, then aim a warming laser to melt them so they bead up, then high frequency nonwarming pulse interval laser to stir them, making a digital gradient of different ion exchange resin beads responsive to a different chemical; a microfluidic channel layer is then printed atop this (or previously beneath) and each of the separate ion exchange chemical concentrators has its own plumbing, at 32 micrometers radius of the bead then there are 47,851 chemically different ion exchange resin chemical separation locations on a 7 cm times 7 cm chip

Another way to make an ion exchange resin chip is

If you liquefy an ion exchange polymer with a laser or ambient warmth then use the actual multichannel microfluidics to transport the ion exchange polymer fluid to a particular well. It is it possible to fill 1 million different wells each with a different chemical component ion exchange resin; blending liquid polymers, using microfluidics, at a variety of ion exchange resin chemical components that are blended together to make an array of 10,000 times 10,000 ingredient gradientized different ion exchange resins, each of which will prefer to glom different chemicals than the other, doing that makes 100 million different ion exchange resins at a chip

It also makes me think this is a possible way to do what is effectively screening a library to find completely new ion exchange resin chemistries, if you then pump in the chemical you want to glom on an industrial scale, and have the microfluidic then sequentially (parallel versions could work) liberate the glommed material you could find the most effective ion exchange resin composition out of 100 million (wells) which is likely much more efficient.

Niftily, ion exchange resins tuned to find chemicals of moderate electric charge and higher lipophilicity tend to concentrate the 100 million chemicals most likely to pass the blood brain barrier and omit macrophage and leucocyte response; that kind of ion exchange resin chip could enrich the number of chemicals concentrated from the homogenate with chemicals that have more favorable drug characteristics which are then higher velocity at being turned into drugs

It could be possible to heighten ion exchange resting chemical concentration 11 times greater than wells with ion exchange resin fluid traversing sponge:

ion exchange resin highly porous sponge, blending saliva with rubber cement automatically makes a sponge so a chemistry of sponge making at microfluidic tube chambers could be straightforward, it is possible that rather than an ion exchange resin bead well you would just make a higher volume tube volume of like a (========) filled with ion exchange resin sponge along the access plumbing microfluidic paths; think of a view like a circuit board multiparallelism with tube balloons periodically, the tube balloons full of ion exchange resin sponge could have surface areas to the 3rd power causing them to glom with 32767 (32^3 micrometers) compared with (32^2 times 3 micrometers) at a well, that is near 11 times more material sorted at a similar surface area but sponge hosting volume

If ion exchange resins can glom 1-10 pct of their volume of product, and 47,851

1/32,000 of a milliliter is the volume ofa 1 cm^2 area 32 micrometers deep, if the ion

1 cm cube that is 2/3 tubes concentrates 1-10 pct of chemical so .067 ml of concentrated material and can be used 11 times, so .67 ml of material

Compared with the 27 item multicuboid concentration and sortation reactor which is utilizes 27 cuboids, each 2/3 ion exchange resin sponge tubes, utilizable 11 to 140 timesreactor

Parallelism is beneficial at chemical sortation and chemistry chips, noting the 1 cm^2 two thirds of it being ion exchange sponge filled tubes product sorter and concentrator, When assembling a cuboid (like 27 packages stacked three on a side) microfluidic chemical reactor or ion exchange resin cuboid form, a Lego (or better) interclick of perhaps 16 tubes per Lego circle thing to make a 27 times capacity reactor or product concentrator/sorter goes with having functional fluidic,and microfluidic connection between the cuboids;

the actually fairly mild 16 times 6 circle tube fittings times 27 cuboids times 6 sides all surface Lego circle connectivity reactor would have 15.5k connections, perhaps better than brickle blocks is tHZ (or, just possibly at some polymers lasers like the 3D shape deepwater laser scanner) weldable cube internals, so the up to 10k connections at a 27 cube Lego assemblable reactor (only about 10k connections out of 15.5k if you ignore the surface) could be Thz welded, puffed to fit, or even capped if leaky, at a yield of just 95% 10,000 connector connectiviyy that produces 95% or higher functional plumbing access piping at the reactor multicuboid. The microfluidics at each cube could have some adaptive routing paths to make all the reactor/concentrator well or tubes accessible. I think making these as spheres is also beneficial

The routing adaptive layer at the microfluidics makes it possible at a 27 cuboid reactor to route the chemical containing fluids clean withe reverse direction flow

Ion exchange polymer reloading as microfluidic pumping of new gradual chemical reaction to make new foam, laser or Thz

it is possible a soft flowing polymer with "brickle blocks with adaptive routing" could avoid the actually fairly mild 64 times 27 times 6 all surface Lego circle connectivity, perhaps better than brickle blocks is tHZ weldable cube internals, so the up to 15.5k connections at a 27 cube Lego assemblable reactor could be welded, puffed to fit, or even capped if leaky, producing 95% or higher functional plumbing access piping at the Lego cube. I think making these as spheres is also beneficial



Integrated circuit technology microstructures; doing electrophoresis at microsized channels that are likely larger than the one million lane microfluidic chip I read about; An electrophoresis gel that is only a gel when it is cooled can be partitioned and pumped around when it gently warms, although there is also the possibility of specific area of the chip warming with lasers; the many lane electrophoresis peltier cooled gel turns liquid at 16° then adjacent microfluidic lanes at 32 ° angles pump the chemical or protein enriched fluid at just that area to a new location on the microfluidic chip; 1000 lanes are loaded at one side of each electrophoretic lane, so each electrophoresis lane at the chip can transport 1000 completely different chemicals like proteins

There many ways to structure the microfluidic channels, some of them remind me of the connected and branching conductive lines on the upper surface of photovoltaic

Looks kind of like (→≠») or ==‡== where fluid flow direction meets a bunch of channels connected at an angle or #€[‡] other branched channel geometry

The liquid sample with liquid electrophoretic gel material that gels at 16°C loads at []‡ or ==‡== then peltier cools the multichannel ‡ to gel, then an integrated circuit, which just possibly is at the base of the electrophoresis chip if it is made with integrated circuit technology as compared with polymer technology or MEMS technology, causes voltage at the long (‡)channels on =‡= causing the gel to sort chemicals, this can all be acvomplished with branches of branches at one layer, or, layer atop ==== has another ==‡== fluid path on it; each concentration at the gel channel can be liquified with lasers; microfluidics utilizes published pumps so above 16° C when the === areas when the concentrated chemical are liquid they are micro fluidically motionized to the perimeter of the chip for further use, alternatively they can just be micro fluidically transported to where the yeast are; at another version the microfluidics transport the electrophoresis products to a bunch of wells on a multimillion well integrated circuit technology like planar area, and then to place the yeast and nutrients, the mechanisms automatically, or if feeling thrifty, a human gently places, or possibly even mists the multimillion well plate that has the plurality of separate concentrated chemicals that the microfluidics have placed at predictable locations and at a database, the actual yeast and nutrient solutio; the wells have the volume capacity to feed yeast for 20 unmedicated yeast lifespans noting that yeast now can be made to live 400% longer (salicylic acid) so that way if the 99.999th percentile of longevizatiom causes a lot of yeast longevity they will have nutrients the entire time.

It is possible putting the entire thing on an ultrasonic transducer will heighten fluid diffusibility

To produce a larger amount of chemicals, utilize some other channel sizes, make the multichannel integrated circuit technology electrophoresis chemical concentrator produce 100 MCG of product per each second or third branch (or layer) of sequentially purer electrophoretic ==‡== channels, 1000 or 10,000 ==‡== functioning in parallel, loaded from the sides of chip with larger distributive microfluidic |[]| perimeter channels, provides higher volume of material being concentrated; there are 2500 preconcentration material loader channels on each perimeter side; 100mcg times 10,000 ==‡== channels processes 1000 mg, 1 gram of sample each full cycle, at 20 minutes per cycle then 72 grams of sample can be separated per day, 14 of these chips stacked in parallel can process and purify 1 Kg per 24 hours



If a yeast weighs a microgram, and it is possible there is a yeast species that massive (1 million yeast per gram) then dosing the yeast at the human equivalent of 2 grams to 1 micrograms human equivalent dose with the chemicals concentrated at a microfluidic electrophoresis chip is possible

Notably having millions of wells at an integrated circuit wafer technology or even MEMS polymer form makes the amount of locations so the yeast can be dosed at numerous different orders of magnitude, about 7 orders of magnitude between 2 grams (phenibut) and 600 nanograms (ethynyl estradiol) human equivalent dose at 8 wells of yeast each to get p

What if the microfluidic chemical sorter had 100 micrometer channels, and 100 of them on each perimeter side that are moving preconcentrated material at each perimeter side of the chip, well that perimeter is a surface area kind of like one cm^2 of motionized fluid; Depth: at 100 micrometers of 16°C electrophoretic liquifiable depth, and 11 minutes per cycle to traverse up to .5 cm (before branching) while having 10,000 micro fluidically addressable tube segment tapping branches (noting the 1 million microfluidic lane chip I read about) then it is like a volume of 1/100th of a ml each 11 minutes or about 1.3 ml per 24 hours. A 7 cm^2 version would produce 63.7 ml of 10,000 sorted chemicals. If less specific lengths of the liquified electrophoretic lane tapping tubes were utilized then you could get larger amounts of 1000 chemical fractions, at 63.7 ml that is 63.7 mg of each of the 1000 different chemicals, although it is actually different than that from solvent mass, so 6.3 to 32 mg per 24 hours could be from 90-49% liquigel solvent

The thing being that the person is producing mg or more of a longevity drug to characterize and quantify at 6 month 96 well plate fish or mice,

If 1 out of every 1000 or 10,000 (different chemical quantities from different resolutions that make .63 to 6.3 mg of concentrated chemical to dose organisms with)

That makes 10,000 or 1000 microfluidic branched channel electrophoresis concentrates, of different numbers of simultaneous chemicals from few or even one to ten times as many from ultrasonicated endolith homogenate. These 1000 or 10,000 chemicals could then be screened knowing, kind of, what they are. Also the microfluidic chip could have 10,000 wells on it so an eentsy sample could be removed if really precise characterization is the thing to do. The amounts, going with the convenient unrealistic presumption of 1000 chemicals of equal amounts is sufficient to dose a mouse with a multidays of dose produced every 24 hours, or at 10,000 chemicals, c elegans. The c elegans could be dosed throughout its life from one 24 hour output. This is

A way to make 99.99th percentile of longevizing chemicals at 24 hours. If age batched c elegans were used for testing then 6 or 9 days would produce a longevity increase %.

Finding the longevity drugs:Use microfluidics to connect the multimicrochannel electrophoresis chemical separator to 100 million yeast colony wells or 100 million human tissue colony wells then use the: the more GFP it produces the longer it has lived varieties of yeast or human tissue culture cells, these emit more light the longer they live then have camera note the most light emitting cells at wells,

It is possible that finding a most longevizing chemical from half a billion different chemicals longevity drug chemical could cause a person to systematically follow twig to branch on a half billion leaf treeee, possibly doing all 29 Binary experiments to find the identity of the chemical. exposing yeast to half a billion different chemicals, then utilizing flow cytometry to find the one with the greatest light emission tells that if it turns the yeast into anendolith lifespan longevity organism it is beneficial to test it on mammals and give it to humans;

Utilizing liposomes with, approximately, just one chemical at them to quantify and characterize new longevity drugs:

With the possibility that a continuum gradient electric charge or pH gradient generation around liposomes, causing them to ensconce different things (or putting phosphatidyl serine and alcohol at an electrophoresis gel that liquefies above 16°C then beaming ultrasonics on the phosphatidyl containing gel produces liposomes to form right there, at a micrometer or eentsier sized piece of a chemical/protein band, ensconcing just that location's chemicals at that electrophoresis band generates a billion or a trillion liposomes, each with mostly just one particular chemical;

You blend the one-liposome-one-chemical liposomes with a one yeast one liposome dilution at a container of fluid, or possibly decanted to make a monolayer; longevity chemicals make the yeast live longer and the most fluorescent yeast has lived the longest, the longest lived 700 yeast are detected with 20 million yeast per second flow cytometry and a numeric characterization of that entire library like endoliths or the kings holly can be made. finding something that makes yeast live numerous orders of magnitude longer and noting flow cytometry lets the yeast live on, many of the yeast remain alive

Then there is a way to trace the long lived yeast to the liposome it ate, and the chemical at the liposome; it has to do with the sensitivity of isotope spectroscopy at flow cytometry, the liposomes can have a large location specifying dose of stable isotopes at them because the electrophoresis gel either migrates a gradient of isotopes with 2-6 different isotopes migrated from each distal part or face of the gel before electrophoresising the screenized material like kings holly or endoliths; so the

isotope gradient, deuterium from one distal part, nitrogen from the other distal part, phosphorus from a transverse side and oxygen from another transverse side; each liposome also then gathers a region associated multiisotope ratio at amounts that can be seen with spectrophotometry of the yeast at the flow cytometer if that narrows the content of the liposome to an eentsy area of the electrophoretic gradient distance (microfluidic is micrometers) then the name of the source and its library of congress number is known and then the process is repeated to find the page paragraph the particular chemical molecule is

on.

Microfluidic version, transport numerous nearly identical electrophoretic liposomes sequentially to the yeast upping isotope dosage two or three orders of magnitude, and heightening actual chemical dose.

Liposomes are make able at different volumes, it is possible that liposomes with 300 times more dosing chemical are valued or possibly really eentsy liposomes with higher chemical/spatial resolution are valued.

possible then when a trillion liposomes form there could be a billion liposomes amongst those that had mostly just one kind of chemical or molecule, the one near them on an electrophoretic band that could be ensconced into a liposome at just that minute variation in isoelectric point, pH, p(some other chemical like NH3) (pNH2), so with a trillion gradient formed liposomes you get a billion mostly one chemical liposomes, (even though only a billion of them are highly focused)

Then After dipping, rinsing, liposome surface reacting, the liposomes with something like ribose or something yeast like to eat, put them at a dilution fluid so the ratio of liposomes to yeast is one liposome to one yeast; the yeast eats the liposomes then You can tell if the liposomes chemical longevizes because things like known longevity drug liposomes make their individual yeast live longer, and the liposomes volume and appetizing coating can be adjusted to get the highest ingestion and longevization response

Does this work with daphnia; could you laser zap an isotope gradient preloaded then longevity drug electrophoresisized gel to make eentsy 11 micrometer sized daphnia food cuboids, then feed them to daphnia to characterize and quantify the longevity effects, doing flow cytometry with spectrophotometry to find the isotopes that describe the location on the electrophoresis gel which tells which chemical book I'd or neighborhood it is from; daphnia are possibly a better longevity quantification organism than yeast.

Could isotope labelled electrophoretic liposomes screen billions of potential antibiotics rapidly, the bacteria that eat the liposomes with the most powerfully antibiotic fluotesce the least when all the nonliving bacteria are orocessed at the flow cytometer. new antibiotics save lives

Grow a sprig of the king's holly in isotopically labelled water, or perhaps do gene therapy on it with isotope labelled DNA, or provide it with isotopically labelled amino acids, or do all three with different stable isotopes; then do electrophoresis like microfluidic electrophoresis or bulk liposomal electrophoresis then when the flow cytometer spectrophotometric thing detects isotopes it is detecting them on actual longevity chemicals



It is possible that some drugs or other chemicals have very high area localization at the brain or body as they are, but that nobody knows of any illness linked to that highly reachable mapped area. Based on how it would likely function to attach another molecule, a drug, to the chemical that reaches that map area they could research the highly reachable areas to find out if adjusting their function would benefit humans, anything from actual medical treatment to causing the highly reachable areas to be what I have read is called better than well

Localization mental illness drugs, keep anti psychotics out of sleepiness areas if they still function

People create ways to keep protein from gunking up tubes, it is possible that unlike proteins, peptides, which can also be drugs, have chemical things which make them much easier to keep ungunkifying, from being able to clean all the peptides off of them off with an enzyme, possibly a deaminase, to drastically fewer gooey nooks at the molecule from having masses and structures up to hundreds of thousands of times less complex, it is possible that peptides screened (or produced) at microfluidics are easy to keep from gunking up areas, surfaces, connectors and geometries that accumulate material, microfluidics technologies at peptides could be clean, fast, durable, and with longer reliability cycles; screening libraries of peptides as longevity drugs with 1/1000th the gunkification at chips could heighten reusability which makes, concentrating, sorting natural peptides better, also absent gunkification the thousands more uses per chip makes it hundreds of times more affordable. From the perspective of making drugs and molecules that benefit people the 2019AD also noting the combinatorial space of peptides is kind of like many factorial and new completely artificial amino acids for peptides have been created, so it is a nonfinite factorial there could be a multihundred times better ungunkiness, tidiness, rinsability at peptide sorting and concentrating chips.

Some peptide libraries that could be mass screened for new longevity drugs: if you divide any protein into pieces about half a hundred amino acids long or eentsier it becomes a peptide so any longevity causing proteins could be made into pieces and screened at yeast in the billions (flow cytometry characterizes 10 billion yeast a second) so if the yeast are engineered to make and accumulate more green fluorescent protein the longer they live, it is possible to find out which yeast have the greatest longevity increase. Longevity proteins could be things like naked mole rat organ homogenate, any electrophoretic band that is at 35 year beavers compared with mice, any electrophoretic band from voluntarily provided, or not-alive person protein differences between humans and other primates, protein bands from termite queens (50- I read, 100 year longevity) not at other termites, the protein band differences between the 17 year part of the cicada's phenotype and its one month long mating form, queen bees and regular bees, the longest lived non s cerevisia yeast and s cerevisia and the briefest yeast, electrophoretic protein bands at the kings holly, 40,000 year lifespan thus far, with the protein not at a few other plants, protein bands at endoliths of numerous species (fungi, algae, cyanobacteria, bacteria) electrophoretic that are not at similar organisms, also noting whale longevity the electrophoretic protein bands at 214 year old whale tissue from non alive whales, or 214 year whale tissue from parturition related tissue in the water, that are absent from other whales; note that there are numerous others and that just these 22 could be macroscopically electrophoresized to get 10-1000 micrograms to dose the yeast with.

Also it would be beneficial when generating the peptides, rather than using enzymes, or even a many enzymes at one flask approach, to divide the proteins into peptides at nonpredictable locations because if you always use an enzyme that divides with lysine then the peptides will all have a distal lysine on them, one possibility is UV radiation, other radiation, or bubbling O3 through the flask

Spectroscopy of isotopically labelled peptides, would a spectroscopy beam at a flow cytometer looking at a yeast be able to see femtograms or picograms of radio labelling from a peptide at the yeast, perhaps, perhaps not, what about a one per 100,000 likeliness of seeing the isotopic labelling and the 20 million yeast per second flow cytometer I read they thought they could make, then that is 200 yeast saying which peptide they have been dosed with per second then that is 630.7 million peptides screened annually, with parallel machines, like 11 of them, that is 69 billion peptides screened for longevity effects. There are 2 1/2 longevity peptides out of that I have heard of, so at an extreme, 1 million published peptides so they might find some from a million isotopically labelled peptides that the first 14 days of spectroscopic flow cytometry of yeast finds, and the flow cytometry of fluorescent proteins notes have highest longevity increase. They could then utilize about 11 96 well plates of c elegans or 6 month fish to quantify the effects on vertebrates of the most longevizing 132 of the peptides.

Use peptide sequencers, and if there are microfluidic peptide sequencers, noting the million channel microfluidic chip i read about, creating combinatorial peptides based on software, like AI, like deep learning AI that predicts effective longevity increase and then makes those longevity peptides which are screenable at yeast.



Precluding protein gunk up at microfluidics, at something like fungal or algal endolith homogenate longevity chemical screening or clam homogenate longevity screening with numerous proteins at the ==‡== microfluidic microelectrophoretic sorter could direct each concentrated, separately tapped band of material at the gel to a microfluidic lane that has an interior polymer (or other material) surface charge that minimizes protein accumulation on the channels sides, at that particular protein's actual characteristics, kind of since you know the charge it is at and responds to you can have it traverse channels where that channel's interior charge is neutral or slightly surface-diffident whichever causes less gunking up

Longevity technology: AEDG (Epithalon) is a peptide published as causing 24% greater longevity at mice, and when administered with thymosin, it makes people 4 times more likely to be alive after 6 years; there are now synthetic amino acids they make, so molecular variants of the A, E, D, G amino acids that are synthetic could be made, assembled into new versions of epithalon and quantified as to longevizing effects; with two new atom swapouts or new atoms per each new synthetic amino acid, that is just 16 binary variants to screen at mice, yeast or human tissue culture, notably this provides a kind of nifty rapid research utilizing human cognition originated drug creation. Noting using a D-amino acid is part of how vasopressin was modified to be orally active a person could enjoy, like, and value making an orally available D amino acid or synthetic amino acid version of Epithalon. Like if I were making a variation, thinking as a human, I would find out if acetylated synthetic amino acid variations on any of the amino acids in AEDG were previously existing, then distal to the part of other things Epithalon they might think Epithalon attaches to or activates things with, I would put the acetylated synthetic amino acid because I think it might cause the acetylated amino acid version of Epithalon to pass the blood brain barrier more effectively, predictably, or pre looking things up online I would find out if achiral (nonchiral) synthetic amino acids have been produced (imaginably a dimer, even like the G at AEDG being doubled, a new achiral glycine)

If this were at a public biotechnology maker space like they have in San Francisco and New York City then it would be nifty to have each of 7 or 14 people each think of their own version of AEDG with the makerspace maker shared enthusiasm and actual craft of making, although possibly just ordering (another pineal gland peptide distally attached to AEDG might have synergistic longevity effects, causing greater longevity increase at mice than the published 24%) things. Also if people at the makerspace knew about AI, or even just the deep learning packages they have, then one of the makerspace people could make a computer program that uses deep learning to create AEDG variation molecules, as well as other longevizing molecules and enjoy doing that, as well as of course wondering how well it would do compared with the human originated versions. If AEDG has a longevity effect on c elegans or more beneficially 96 well plate 6 month fish, then 3 of these plates would give results on the 7-14 people's AEDG versions times 2 or more variants each, utilizing 8 organisms to get a p value, that is 224 wells of fish as well as c elegans to quantify all 14-28 variations.

There is also screening a library to find a more longevizing version of epithalon, from a synthetic amino acid perpective, Modifying the four amino acids to have four atom swapouts per amino acid then that is 2^16 screenable variants, near 65,536 versions; going with the idea they could modify the Epithalon on purpose they could make eight atom swaps or additions each at the two amino acids on the side of epitalon that actually activates things (if there is a receptorside, make the two groups of four atom swaps at the receptorside) then these 65,536 could be screened at yeast as well as human tissue culture to find 99.9th percentile of greater longevity increase from the molecules screened. I do not know if Epithalon makes c elegans as well as daphnia live longer or not, if it does then these could be utilized to compare different AEDG, Epithalon variants.

I perceive Epithalon might be tolerated at a multi-order of magnitude dose range, if it is then that suggests something Epithalon activates gets fully activated, kind of saturated; finding out what that saturated thing is, then making more of the saturated thing, or possibly supplementing, as a longevity drug, the amount of the saturated thing could be a new longevity drug; also i do not know if the saturated thing is a cell type, a receptor at the brain (CNS) and at many places at the body, or a chemical. If it is a cell type or receptor then the genetics of the saturated thing like SNPs, alleles, or copy number variations could be part of the genetics of greater longevity; if it is a cell type or receptor then the epigenetics that effect the amount of DNA transcription that effect how frequently or how many of that receptor gets made could be longevity heightening epigenetics, some epigenetics effects function at multiple generations of humans so it is actually possible taking a tuned HDAC inhibitor could turn making lots more of the saturated thing on, or make lots more of it, and that the heightened longevity effect transfers to your children and grandchildren.

Does making BDNF nerve growth factors or other growth factors at the pineal gland heighten longevity and healthspan.

Other pineal hormones, Epithalon, to my perception was developed from researching pineal peptides, it is possible some of the other pineal peptides are more longevizing but at the time a four amino acid quadropeptide was particularly easy to purify and make, it is possible there are lengthier amino acid sequence pineal peptides that can now simply be ordered to be constructed online and screened at mice to find out if they have greater longevizing effects than Epithalon

Does a 300 year lifespan tortoise have a pineal gland? What about a century bird lifespan bird, could a recently alive, hospice-care like administered multicentury lifespan whale's pineal gland have its peptides sequenced, all of these pineal peptides could be tested at mice to find out if they are heighten longevity above epithalon's published 24%

is it possible to grow stem cells from a multicentury lifespan whale, possibly using traces of GI tract tissue at whale poo as a source, or a plucked hair, and then differentiate the multicentury longevity whale's stem cells with tissue culture to make a source of whale tissue to develop longevity drugs from.

Do 300 year tortoises and 300 year sharks have pineal glands, if they do then their likely varied pineal peptide sequences could be characterized at mice as to longevity increase to find a peptide more longevizing than AEDG, epithalon

Longevity genes and proteins at multicentury whales: at humans, HARs, human accelerated regions are areas of the human genome that have changed and diverged with the most velocity, possibly as compared with other primates, humans living much longer than another primates could be linked to HARs, so at multicentury whales do they have cetacean accelerated regions compared with other whales, if they do then genes causing greater whale longevity could be located among these CARs, humans comparing those CARS, as well as human HARs To each other might find shared homologous genes of longevity (compared to nonhuman primates) with the multi century whale versions of the homologous CAR and HAR genes shared areas being particularly longevizing, the actual gene products, like proteins, of the longevizing versions could be heightened at humans as a longevity technology as well as CAR HAR most longevizing genetics gene therapy, germline gene modification, longevity drugs, it could also be that Whale CARs (comparing nearest genome at other whale species) have a completely non homologous to human genes longevity heightening gene area that could be used to develop new longevity techology as well as longevity genes as well.

CARs and HARs as sources of longevity genes, could be sources of longevity genes that benefit humans even when they arise at other species, imaginably noting epithalon's 24% longevity heightening effect, and that i think brain development areas are among the human accelerated regions, HARS, it is possible if you make pineal effecting HARs genes part of the mouse genome the mice might live longer

Complemtary

Thinkable: noting three of them as a proof is it possible, along with longevity with healthspan and wellness and being simultaneously benevolent and beneficial and effective at raising children, that there is anything at humans where a larger amoumt of it is always beneficial? This could actually have a mathematical interpretation where some axioms about iniverse math and topology create different and beneficial math limned options;

Getting around all the math of how varieties of sharing can occur at different math systems, dividing odd numbered things on a curved surface or a planar surface causing equal, unequal, matrix equal but anisotropic kinds of partioning, as well as the mathematics of nonpartitionable systems, also interconnected or stand alone systems where all agents always have perfect information arising from the math definitions of the system they are at, consider a person with 360 spherical vision living comfortably in a pleasant easily climbable bowl, who has used science to verify that like 20th century humans communicating the, to my perspective, MWI modified idea, that there is no thing space expands into; the person with the 360° spherical vision actually, at that math space, sees it all, and has the mathematical topological space of having perfect information; the "outer surface" of the bowl is absent detectability with physics, and to have the math function with everything that has ever been measured supports there being no exterior of the bowl (gideon's trumpet, nonfinite length, enumerable surface area possibly is reminiscent of a bowl with a math space where there is a region of presence and non presence at a thing); finding new math spaces that optimize human well being is beneficial, because they make worlds where polythings align with goodness

With math as a beautiful prompt, are there things that if there were heigtened amounts would always be beneficial; well i think it is possible to create a math space where kind of like a topology of all beings having perfect information (360° spherical vision unisided dish), any recombination always produces a more beneficial change in form than omitting any kind of recombination or sorting, while the previous to change universe, at that mathematical topology, is to any sentience fully optimal; any change could heighten an aleph number that a mathematician describes the universe' topology with, one possibility is

So thinking about where i have presence of being, is there a heightened amount is always beneficial

If i can just make atoms, is that a more is better thing, anyone can use them, and noting that the universe is good it just causes a greater amount of universe, using math the new atoms could have equational creatability at various equational areas of the universe, sort of like the way mathematicians are able to definitionally limn an area where "you can add 2 to anything and it will retain its odd or evenness" there is a math system like a topology where it is possible to put more atoms, at a kind of

This is different than the kind of langiage effect "can i find an exception" this is making new math environments where if you say torus then another person saying, thete are two or more circumferences is the way it goes( there is an exception though, if you put a gravitational singularity at the middle of a torus then that middle circumference ballons towards it and at 1 dimensional numbers a balooning occassion causes the middle circumferance to be identical to the outer circumference;

A computer scientist might say, oh, what you mean is algorithms with proofs that limn their behavior, and, among other mathematical forms, i think you would like thinking of mathematically limned "proofed" algorithms that, noting the universe is good, make the universe gooder, so you are utilizing a moment to think of thimgs where at any mathematical or definitional space a greater amount of any anything is gooder;

Whether it has an aleph number or not heighten the universe' aleph number

Just make more atoms, is there a way to figure out if just makimg bosons or just makimg fermions is gooder, have you asked aroumd about a greater quantity of spontaneously resolving quantum objects or also a greater quantity of quantum objects that are unresolvable

Is there a gooder quantum object like a linear photon that gradually builds its brightness so that any organism that liked it could experience it, or if they felt sleepy could just shut their eyelids and go to sleep, , or a true analog thing that is. Planck unit nondependent

If you have a network of n objects such that when superimposed the additive wave is always positive and high thing to the³ compared with thing² and the top half of ac is at an etalon and the container size and when I say container it could just be a parabola of frequencies with the upper part of the parabola a wave frequency larger than the span of the universe and the optics such that only optimal images would retroreflect

A parabolic mirror at a math space where everything was reflected twice at a frequency doubling crystal such that if it were reflected once it would traverse to outside the network and diffuse to the same frequency as the nearest most light emitting object, then shape the topological space so that always happened, all the images viewed would be right side up and the right handedness would be at each image the reflections would be a clearer image each time because they are vector graphics, a casimir flashlight could provide the light that gets

Casimir flashlight automatically motionizes vector graphics

Casimir flashlight attached to an oscillator always gives the oscillator a nudge with some surplus and the surplus makes the parabola higher and raises the curve at the base or at a math space does the up widening traverse thing as it is topologically gyreless, the parabola lifts at the same velocity the math space expands so the proportionate location does not change while the vector graphics reflect twice all the time with the raising and expansion causing a larger number of vector graphic reflections of higher resolution while (at a size changing topological universe)

This could also be accomplished with a gradient refractive image lens at a plasmid shaped topological universe, any occurrence at the sides of the lens would be at total refractive index, and the network nodes at the interior of the tir would experience connectivity without reaching the perimeter; an optical computer in the middle of a curved optic with 3d hat wave, egg cup, such that the tir caused retro reflection that was also a vector graphic; so what the network experiences is heightening vector image quality as the parabola lifts and heightens while the nonperceptible tir at the sides of the plasmid expanded, the duration of the reflected vector graphics is such that any node of the network was equidistant from a light patterns gradual and normal pace versions, the cofocalization of the sides of the parabola would cause the connectivity of the network as well as the nodes to accumulate presence of effect in phase with the network nodes that were around, with math I think you can have a simultaneous group of equations that does that, casimir flashlights work in water and the light would accumulate in place if there were no nodes, noting million year endoliths the nodes would usually be there and they could process the vector graphics from the sides of the parabola or each other the casimir flashlight could actually be a coating,



Thinking about math, although i perceive observing a new gooder thing and heightening the quantity is a gooderness maker

Is it possible; what is a way to observe a new gooder thing?

Well, you could find people that observed new gooder things, then with technology simulate them nonsentiently then actuate a trillion of the nonsentient simulations, then broadcast the new gooder things they observe at a math space of

Simultaneous linear equations of the amount of streamlining, also words that happen at my mind that are 40% or less of what i actually thought, although these 40% words may have a participateable theme of their own, intimations of pattern meaning from form being different

A drug that decreases streamlining some number of units, while being actually recognized as heightening absence of streamlining whete teduced externsl patjwayization is like 900 units of less automatic so another person bringing up an opportunity for a voluntary action would have 90 0units of greater possible voluntaryness this simultaneous equation space where automated respone forms habe less prompting while a new beneficial thing is questing agter psrticipation contributes to a mathematics supporting the possibility of voluntary action (like math supports physics) notably there could be many such drugs which happen to cause gooderness at most of the cumulative occurences, also there are some drugs that cause, at me, a high, while also decreasing streamlining and possibly 40% thoughts, a drug that might do that is phenibut, a nonintoxicatimg drug that removes the abridgement of actual feeling and thouhht of intent 900 units while a different benevolent thing is alignig 900 units of voluntariness as utilization capacity with the change like a beneficial thing, they favor

Genes of openness to experoence; genes of the words in your head being some percentage of what you mean, like actually 100% people, 90% people, 40% people being genomically compared and then their genes used to predict the statements about"words in my head are(amount)% that I mean", that a sample of people with genomes at a database make; when the computer model of the genes that cause some version of this is 95% predictive then the genes of 100% of the words in your head actually mean are promoted, perhaps even made available as a sample with gene therapy or drugs, then to the amount they can, people can purposefully opt for, along with their previously actually knowing what they mean, what they thought, and perhaps for some what they intend, the actual words in their head, like casual narrative thinking, such that then articulate that

It is my perception that the debrisifying or content mutation or content simplification that occurs when the words in my head are at a 40% thought that the actual capacity or ability to be simultaneously cohered at a voluntary action opportunity, so it is more actually voluntary like numbers derived from simultaneous linear equations solved amounts of the possibility an act is voluntary gets greater the more the words in your head are 100% what you mean;

Somebody reads an advertisement for something that is actually beneficial, if the words in their head are 100% of what they mean, and they actually get the beneficial advertised object, then their capacity, like their available amperes of voluntary, is more voluntary than a words at my head communicate only 20% of what I mean person, so this is genetics and drugs that heighten the capacity of humans to be less encumbering to others sense of wondering if they are being fair, if a 100% person volunteers it may actually be more ethical quantified like amperes (compared with ethical the dictionary definition)

genes of

It is possible a nuclear chain reaction, noting it always decoheres more things than it resolves, continuously might be a simple sytem that is unresolvable, a person could only ever say they saw part of it; also some of them are autocatalytic; what is the eentsiest always quantum unresolvable system that humans also like, white light, being physicsy, is definitionally omitting of sufficient observation to be described as its components, although there could be a math thing, or even a physics thing like we emitted some white light so we know how many watts occured,

Stomach lining 72 hour rrgrow

[Treon Verdery]

Klotho is a kidney gene that makes mice live longer and be twice as effective at finding things when genetically engineered to make a lot of it , it is possible that organisms that utilize their kidneys and brains a bunch simultaneously could have more effective versions of klotho, dolphins, whales and sea otters are organisms that may have this feature from gulping ocean water and processing it, beavers might gulp a lot of freshwater and as they build things may benefit from higher cognitive ability, klotho could, possibly or possibly not, contribute to beavers living 35 times longer than mice, squirrels which have orders of magnitude greater ability at remembering and finding objects, to my perception, compared with mice I previously mentioned as Klotho gene versions, with possible optimizability, as well as slight variations on the gene protein product as a protein drug, a longevity drug as well as a variation as a protein drug nootropic

Longevity technology

Klotho external protein is mentioned as receptor less but concentrating at a word like sialogangliosides, that suggests sialogangliosides could be tested as longevity drugs and nootropics

Supercentensrians were tested and found to have about 1.5 times greater likeliness of having the most favorable variant of the klotho gene

[Treon Verdery]

[Treon Verdery]

With higher IQ linked to greater longevity they could do brain scans of super centenarians and find out if their actual brain mass at each millimeter of brain structure varies from others, quantifying positron emission tomography amounts of radio labelled chemicals the supercentenarian variation at what amount of actual neurotransmitter chemical is where as well as neurotransmitter receptor density is describable, and could be associated with longevity, and longevity chemicals active at mouse brains, complementary to while being different than nootropics could be screened and produced; non cognitive areas of the brain could also be heightening longevity (Epithalon, a pineal peptide, is published as causing 24% greater longevity) new longevity drugs developed to address brain areas that might be outside cognition are also beneficial



Screening stimulant molecules to find out if there are longevity heightening molecular variations:



On finding 99.9 th percentily of longevity heightening stimulants at 96 well plate fish, screen the 99.9th percentile stimulants on mice, quantify absence of emotive or also social conflict, optimally less than the amount of median unmedicated mammal emotive and social conflict, also beneficial is quantifying mouse actions, communicating, feeling happy, play, and willingness to travel at open spaces, another the benefit of a longevity producing stimulant could be quantified conviviality, also mating frequency,



Stimulants at less than a behavior changing dose could also cause greater longevity particularly screened as a library at yeast as well as c elegans;



At perceptible stimulants a voluntary human experimental participant verifies the longevizing stimulants as pleasant and fun then marmosets at age batches verify longevity effect while having fun



It is beneficial to

Screen longevity stimulants as beneficial or neutral to the fetus, or are also absent passing the placenta



Rapamycin is published as causing 60% greater longevity as well as higher cognitive performance at 126 ppm at mice, I took some at kind of near 24-63 ppm and noticed it made me kind of excited so I did not go to sleep, so I started taking it, when possible, before or near noon, it is possible that screening a library of rapamycin molecule variations to find nootropic or also beneficial prosocial stimulant versions could be even more longevizing than rapamycin (60% rapamycin with near 20 from IQ is 80 pct longevity heightening) stimulant ability of rapamycin molecule variants could be screened at c elegans to see how fast they move around before quantifying the effects on longevity, social behavior and cognition at mice;

attaching a nootropic or stimulant moiety to rapamycin could put the 21% greater longevity from higher IQ with the 60% longevity heightening from rapamycin to make a drug with 80 pct greater longevity effects that could be quantified at age batched marmosets



Epinepherine I heard, is aversive, it might be linked to heightened cognitive function as I perceive I read some humans get hyperfocused, more simultaneously aware, and mentally and physically faster possibly at a voluntary epinepherine producing activity, screening a library of numerous epinepherine variants could find a fun, harmless, pleasant, peaceful, multiarea beneficial longevizing version



Organoids as tissue culture forms that could be utilized toscreen chemical libraries, positron emission tomography on tray stacks, 3-7 mm per well, 49 rows columns and stacks per positron emission tomography scan occurrence scans 117,000ish verified as being absent isness organoids



Is a way to verify absence of presence of being, like at 3mm neuronal brain-like nuggets like organoids to position the thing being qualified as absent presence of being as a quantum system observer, then if the system is observationally resolved there is possibility of presence of being, if the thing is absent quantum resolution then it is possible the object being qualified as to absence of presence of being like an organoid is either not being attentive, like a human that is so romance focused they fantasize about their girlfriend while looking at the quantum apparatus and do not notice what is going on, or rather than nonattentive it is possibile the organoid is actually absent presence of being, while quantum resolution as compared with a 3mm nugget of a different tissue would make it so they built different organoids that were absent quantum resolution capability



organoids with reduced likelihood of having presence might be built with a matrice of neurotransmitters, neuron types, or morphologies omitted; screening things at organoids matricized to have serotonin with acetylcholine but not dopamine at one group of organoids, while a different group of organoids has a different neurotransmitter matrice, causes nonbeing form when screening a million organoids at chemicals like beneficial longevity drugs;



Longevity technology as well as new longevity drugs

[Treon Verdery]

Longevity technology

I read human Eunuchs live 19 years longer than other people, plasma mass fractions and tissue homogenate chemicals from cow, horse, pig, or rat eunuchs as well as ordinary organisms could be compared to find chemical differences, then the different chemicals administered to c elegans to find out if any had longevizing or modulating effects, also noting an old rat and a young rat with connected circulatory systems causes greater rat youthfulness (as well as possibly longevity, I do not remember what it said) they could test eununch rats with their circulatory systems linked to ordinary rats of the same age to find out if there were longevity effects,



similarly there may be longevity chemicals sequential to the mTOR effects of rapamycin, published as making mice live 60% longer, connecting the circulatory systems of old rats that were, until 54 hours previously, on rapamycin, to those of old rats of the same age could find out if there were non mTOR longevization chemicals circulating at the previously rapamycin treated rats



The difference between the human age equivalent of 60 year old normal rats and 60 year old multimonths of previous rapamycin, rapamycin treated rats could be characterized as to their electrophoretic physiochemical difference, and the notably different chemicals tested on yeast and c elegans for longevity effects



The electrophoretic physiochemical difference between 30 and 40 year old human equivalent age rats and rapamycin treated 60 year old equivalent rats could be characterized, the most maintained physiochemicals from 30 year old to 60 year old rapamycin treated rats (as compared to 60 year old untreated rats) these physiochemicals could be tested on yeast and c elegans, if large mammals on rapamycin are utilized then that could be a plentiful source of chemicals to test mice or shrews with









The difference (a list of only which are shared) between all the chemical differences between young and old test mammals, and eununch test mammals could create a more rapidly testable list of chemicals that heighten longevity



Electrical engineering technology



Mechanical engineering technology

Standard sized containers at trucks effect the size that machines loaded on them can be, doubling the length, height and increasing of those containers without going to specialized "oversize" flagging would make it so larger machines, machine parts and other things could be transported, it could be that driverless automated trucks could transport these larger standard containers with minimized (less than human) risk, and stay away from other drivers, also, possibly adjusting velocity or idle time to drive when the fewest other cars were around is possible, that would cause new efficiencies at distribution



Trucks, like driverless trucks or other trucks could have weights placed at the bottom of the wheelframe or carriage to make double stacks of containers compatible with stability



I have seen bread trucks three containers long, double stacked containers at these would make them 6 times more efficient than one truck carrying one container, it could be that one or two powered wheels more at the second and third trailers would make it so driverless trucks could do the physics to do this harmlessly, the minimal size, minimal weight one is sufficient wheels could be electrically powered from the engine on the trailer-truck



Electrical engineering

a new way to find higher performing components in a batch, on part of an assembly line they could have an addressable conductive fabric under every item, the items could have their own individual id number and every part tested to find the 95th percentile of overclockability, leading edge cleanness, and at analog parts onspecificationness, these could be pulled aside with their individual visual identifier and described as a separate product



Longevity technology

Utilizing green fluorescent protein to describe how long an aorgsnism like yeast has been alive, when genetically engineered to make GFP, the longer a yeast has lived the more GFP it produces and a camera can scan a grid of wells identifying which one has the longest living yeast, similarly at c elegans this is possible, engineering yeast and c elegans to make a couple other colors of blue and a couple other colors of green could track multiple metabolic activities of the c elegans simultaneously, if it is possible to look at muscle mass or something that accumulates with motion then the amount of motion might be associable with wellness or healthspan



At c elegans it might be possible to genetically engineer a near transparent variety that made green or blue fluorescent at its heart so the 96 well plate scanning camera could tell if the heart was still beating and if it is still alive, this would get rid of having to physically probe the c elegans to see are alive, gentle ultrasonics, or infrasonics has been previously suggested as a way to do this as well





Things that fill children with happiness

Greater tolerance of child like behaviors at school could make children happier

I think some of children's actions make them happy, perhaps sometimes with an immediate focus, while sharing and teaching reading one of the students picked up a chair, put it on his head and twirled it around, but his facial expression and vocal tone were neutral, it could have just been being lively, I thought it was a fine thing to do, I thought about what the kindergarten teacher might think, at a different environment other children might have imitated him and some chairs might be audible or provide distraction to all the students in the room simultaneously, if it takes the teacher 3, 7, or 9 minutes to get all the children to recommence their learning, and this happens twice a day then that is about 4% of the days education missed out on, i think it is possible to make education much more effective, and just 4% greater improvement among the much greater improvement possible would make it possible to tolerate more childish behaviors. It is possible that tolerance would make children happier. So for every 14% increase in educational efficiency and then 2% of the gain is utilized at tolerance of noneducational behaviors then a 100% increase in educational effectiveness would be 14.2% greater time children's noneducational behaviors could be recognized tolerated while maintaining notable gains in educational effectiveness. That is about 8.52 minutes of extracurricular activity per child per hour, if that chronological interval were utilized to make children happier, then children would be happier, the thing is, what is the way to double the effectiveness of education. Terence Tao has an IQ of 200, it is likely he learns more than twice as fast as others, germline (germiline is oocytes and sperm) gene modification to make people, that is persons, that is humans, that is homo sapiens 3, 4, 5, 9, 10, 11, 20, 21, 22, 23, or twenty four times more intelligent than Treon Verdery beneficially multiply the effect of education and causes the amount of tolerance of children's behavior to go way up, making them happier.









Longevity technology

Human growth hormone is associated with greater wellness and I think healthspan, but not longevity, screen a million molecular variants to find out if any cause greater longevity at yeast, a few thousand variants could be screened with the outdoor mouse dorm. Also human thought could go into localization of human growth hormone to concentrate it at certain areas (possibly CNS and muscles and peripheral nervous system) and away from areas it might be deleterious like the cardiovascular system. Putting localization moieties at the human growth hormone protein could facilitate that. Also there may be SNP variations and allele variations among humans. Among supercentenarians over 115 those gene variants that contribute most to healthspan and wellness could be considered, thoughtfully, and the effect on the lifespan of the entire human distribution considered before making drugs and optional gene therapies, there could also be a drug addressable epigenetic component



Semiconductor technology

It is possible that some integrated circuit materials have higher feature resolutions than 3nm or 1nm, making things out of those materials with regular engineering compatible feature performance that is eentsier than the entire full range of semiconductor materials is beneficial, perhaps elements like K and Na coated with larger SiO2 would omit reacting with anything but still have utility at MEMS or even conductive pathways between other features



Photonic technology



Genetics of being an autodidact. It is possible being an autodidact has a genetic component, they could look at the genetics of autodidacts and find out if anything is associated with it, psychometrics might find cognitive components of being an autodidact and they could find out if the components had separate findable genetics and then find out if people with all of those separate components at the population were more autodidactic, then it could be a beneficial part of the germline at all humans



I favor children being taught they have a right to turn machines off. I favor making machines so beneficial, and at robots that emulate personality, so benevolent children and adults seek them out and turn them on and keep them on.



I favor all artificial intelligence and robots be nonsentient.



Longevity technology

Are there oceanic fungi, screen them, as well as their chemically separated homogenates for longevity chemicals with yeast, they may have notably different genetics and if collected at cold water, sparse nutrient areas could have much longer lifespans, that could also function with cold water sparse nutrient ocean bacteria



Things that fill children's lives with happiness

It is imaginable that children that get an optimum duration of sleep with various optimal chronological amounts at different sleep stages are 7-19% happier (imaginably, as based on adult experience). A bed or camera technology could measure sleep behaviors and duration. Then software like deep learning AI that had learned what amount of sleep, at what age, at what genome and psychometric data, would then communicate to the child and parents the benefits of different amounts of different amounts of, and kinds of sleep. It is possible there are gentle things that beneficially prompt different sleep stages to optimize them, like a meal before bed, nonlyrical music customized to each sleep stage while asleep, the lighting between bedroom and bathroom, a blanket that cools or warms, the presence of a body pillow as well as other things could be quantified as to the amount they modify sleep stage amount and duration to make the children happier and more well rested. This could also benefit adults.



Children's shoes could have pedometers in them to measure activity. Software or deep learning AI suggests an optimal amount of activity, sometimes an increase, that causes 99th percentile happiness increase from the pre-pedometer measure of happiness



How often do people change their routine, and does that trend toward optimal, if a person always changes their routine towards something more optimal it is possible things become cumulatively better. Software could advise people on their routines and deep learning AI could look at those at the 99th percentile of happiness, doing what they value, productivity, to find routined other people can make use of. About half of people are MBTI J and might gravitate towards routines



Semiconductor technology

Surface mount IC technology, pick and place robots could stack SMC components on top of each other, saving board space, having 14 micro contacts per upper surface, and having any one or few of them sufficient to carry the current, while engineered to contact at all 14 heightens reliability, if metal Velcro is a thing then pressing them together omitting solder could be possible and adhere to engineering specifications, nonoxidizing metal Velcro heightens reliability and lifespan



MWI technology

Whenever you search for something and click on a search output item the software opens up an extra browser tab from finding the most benevolent and beneficial result (thesaurus similar meaning words) on the third through 9th pages of results, sometimes people will view it, sometimes it will make them think, and sometimes they will learn about a less SEO optimized, less mainstream thing about what they are searching about, it is possible it will prompt benevolence and benefit, at the MWI these could be different sites informing people of different things and causing different knowledge and different actions with a tropism towards benevolence and benefit from parsing the link descriptors, this is a browser add-on with mildly white and blue tropism



Regoogle, it makes Google or Bing better from parsing the first 24 pages and uses deep learning to put half of what the deep learning AI on your computer thinks you will like and half beneficial diversity, among those search results with the most benevolent and beneficial thesaurus words



Software browser add-ons that address the 9 most popular sites and Imgur (a meme image site), deep learning AI at the browser notes the time you spend on each imgur image (rapid click through suggests lack of interest, mouse movement to read text and comments suggests high interest) then with that data sample gives you the ability to view "My Imgur", that is graphic memes the AI thinks you will like based on previous dwell time at other images and deep learning AI' noted image matching ability as well as text cues like body text and favorable language comments, this could be a part of ReGoogle or ReBing which could decorate each page with a graphic, a meme the AI thinks you will like, this could be modified at the configuration menu







Concentrating elements from seawater with microorganisms, is it possible to make spongelike charged proteins, possibly spaced beta sheets or similar with charged amino acids, or even more highly charged synthetic amino acids, that microorganisms could make, and at better technology move from the endoplasmic reticulum to the cytosurface like receptors, perhaps existing receptor genes could just have codon sequences that say make a bunch of ion-exchange resin functioning-like proteins at a location at the existing receptor gene and it would get transported to the cytosurface



It occurs to me that at 2019 US homeless persons were about 1 out of every 300 people. At nations with just 10 million people philanthropists could provide photovoltaic Costco huts ($1200), with portable toilets, continuous video and audio at the area, lighting and a portable shower building for their 33,333 homeless people (or fewer depending on the country) for about 69 million US $ at $2000 per person. There could be a simultaneously utilitarian method, this could be combined with the vacant commercial land being developable into the riskless, financially neutral, generator of greater numbers of parks method, change to the law. Another simultaneously utilitarian way to do this is credit unions voluntarily setting aside .01% of the accrued interest on typical US 250k-300K dwellings to finance a $2000k Costco mini hut dwelling for the homeless. The credit union could then use the projected value of each $250-300k dwelling loan with factoring to create an immediate amount of money to order the mini-huts. This could have advertising appeal to banks, at 2019, "finance with us and provide a homeless person with shelter until 2062" (42 year durability of Costco mini hut). The combination of 4 out of 100 people financing through banks and all the people financing through credit unions would generate mini-huts from 7-14% of all dwelling financing. At the US with about 79 million 2019 financed dwellings value 21.7 trillion at 7% voluntary participation and .01% interest is 1.5 billion $, that is about 2/3 of the amount required to provide minihuts for all the homeless people in the US, at a 9.3% participation rate the full amount is covered. I read people in the US move about every seven years, if so then it is possible every 11 years 79 million dwelling refinances occur (some people do not move), which makes the process take that long, and much more rapidly at some areas (credit unions like to reinvest in local communities, banks can remit funds where the opportunity is greatest). Also, it could be a standard part of the credit unions' and banks' package for people opting for the dwelling for homeless program, the financing corporation could provide a "this months interest rate or next months interest rate whichever is lower" standard option, half of all the people financing dwellings would gain lower interest rates a month later making their financing more affordable.. The financial companies could figure out the difference between the two interest rates dollar values, perhaps 1-2% change at the initial interest rate, so perhaps $20-40 to cover the difference for one month, if the banks charged $60 for the lowest interest rate occurring over two months option they would earn 33-300% profit from making the product available at that fee, and more if they rolled it into the dwelling loan. There is however the value to the bank of immediately transferring the financing to another financial company and getting money up front. Of course a "this months interest rate or less" financial product could be created even without the dwellings for the homeless aspect. Another possibility is that people nice enough to opt for the dwellings for the homeless financing package are quantifiably better credit risks and so the .01% is covered with the reduction of risk to the financial institution. That would be functional up to median credit risk, possibly causing as much as 49% participation. I read about a philanthropist that gave $1 billion to a form of government and they could have done something more beneficial.



Things that fill children's lives with happiness



Longevity technology



MWI technology

[Treon Verdery]

Royal jelly is published as making mice live 25 (27%) longer, and queen bees live about 36 months, 12 to 24 times longer than other bees; noting that insect chemicals can cause mammals to live longer, it is possible other long lived insects could have chemicals that cause greater longevity. The mice that were longevized with royal jelly were fed it orally, bringing up the possibility of change to the proteins in it; some insects, queen termites, live 50 years, with some possibility of 100 years, feeding ground up insects to mice, both orally and as enteric capsule or coated material could find out if the ground up material of long lived insects cause greater longevity; another approach is to extract circulatory fluid (hemolymph) from these insects and find out if it makes yeast or c elegans or fish live longer, notably, fish as vertebrates could be notably suggestive of mammalian activity; another insect to screen for longevity chemicals are cicada nymphs (17 years), beetles of the genus Eleodes (up to 17 years)



longevity technology: I perceive I read playing back EEGs causes the brain to be better at learning and can reactivate emotions; it is possible that rather than EEG, electrical recordings from the surfaces of children and 16 year olds could be played back at the body surface to find out if it has any longevizing, wellness, or healthspan effects; this could be tested on nude mice, and might go well with the body and tissue thickness of mice as proof of concept; at humans something like a wireless recharging near body surface implant (kind of like a pacemaker) could provide the youthful EEG-like stimulus, notably it is possible that wave and frequency variants like nodal, antinodal or possibly depth-reaching electrical solitons https://phys.org/news/2006-05-solitons-electronics.html (dissipative solitons, 100 times farther travel than regular solitons) could be used; notably, some current levels and alternate voltages for the EEG-like frequencies could be used to possibly reach tissues at greater depth, as could wrist-to-wrist or leg to wrist, or even head to calf electrode placement



EEG playback technology: playing back EEGs to cause beneficial cognitive effects using different voltages or currents to reach deeper into tissue, notably though some currents and voltages might verge into tDCS (although AC-like) which is a different thing; the previously described gelatin capsule sized piezoelectric frond hair adhering and head skin seeking technology could also do some tDCS as well as EEG recording, playback and software based synthesis; children’s EEGs could also be played back at adults to find out if there were child-mind effects as well as, just possibly, longevising, wellness, or healthspan effects (seems unlikely, but is possible), notably children have more delta waves, and these occur during sleep, although less so at older people, these child EEG frequencies could be played back while adults sleep; this could be tested on rats, or some larger animal like cows, sheep, or horses



Longevity technology: Some biologically occuring tings do rapamycin like things https://www.ncbi.nlm.nih.gov/pubmed/29165314 It is possible that mutating these plants, then screening their chemicals on yeast and things like c elegans with 96 well plate technology could create much larger rapamycin like longevity chemicals at plants, these could then be genetically engineered into delicious as well as ubiquitous plant foods to create longevity producing fruits, grains, and food products like pasta, pizza, potato products, breading, there is also the possibility that moving these gnes and gene products from plants to eggs and milk could be beneficial



a molecular variant or rapamycin, rapamycin preassociated (attached) to a protein makes it 2000 times more active, “To probe the affinities involved in the formation of the FKBP.rapamycin.FRB complex, we used fluorescence polarization, surface plasmon resonance, and NMR spectroscopy. Analysis of the data shows that rapamycin binds to FRB with moderate affinity (K(d) = 26 +/- 0.8 microM). The FKBP12.rapamycin complex, however, binds to FRB 2000-fold more tightly (K(d) = 12 +/- 0.8 nM) than rapamycin alone. No interaction between FKBP and FRB was detected in the absence of rapamycin. These studies suggest that rapamycin's ability to bind to FRB, and by extension to mTOR, in the absence of FKBP is of little consequence under physiological conditions.” It is possible some molecular version of rapamycin (a rapalog) could cause mTOR activity without FKBP protein, FKBP has immunoeffects so that molecule could have less immunoeffects than rapamycin



Rapamycin that is more water soluble, “clinical development of its formulations was hampered due to its poor solubility and undesirable distribution in vivo. Chemical modification of rapamycin presents an opportunity for overcoming the obstacles and improving its therapeutic index. The objective of this study is to develop a drug-polymer conjugate to increase the solubility and cellular uptake of rapamycin.

METHODS:

We developed the rapamycin-polymer conjugate using a novel, linear, poly(ethylene glycol) (PEG) based multiblock copolymer. Cytotoxicity and cellular uptake of the rapamycin-polymer conjugate were evaluated in various cancer cells.

RESULTS:

The rapamycin-polymer conjugate provides enhanced solubility in water compared with free rapamycin and shows profound activity against a panel of human cancer cell lines. The rapamycin-polymer conjugate also presents high drug loading capacity (wt% ~ 26%) when GlyGlyGly is used as a linker. Cellular uptake of the conjugate was confirmed by confocal microscopic examination of PC-3 cells that were cultured in the presence of FITC-labled polymer (FITC-polymer).

CONCLUSION:

This study suggests that the rapamycin-polymer conjugate is a novel anti-cancer agent that may provide an attractive strategy for treatment of a wide variety of tumors.” hydrophilic rapamycin combined with regular rapamycin could reach a wider variety of tissue types, combined they could cause greater than the 60% longevity increase published at mice

Optically activated rapamycin, “We developed an optically activated rapamycin dimer” perhaps a topical benefit

focusing rapamycin effect on just one tissue (kidney), “Thus subcapsular delivery of rapamycin-loaded microspheres successfully inhibited local fibrotic response in UUO with less systemic effects. Therapeutic effect of released rapamycin was most prominent in close vicinity to the implanted microspheres.”

longevity technology: Liver function genes, like SNPs, could be a source of new longevity drugs, gene therapy or also germline gene modification, “To identify the pathways that could be responsible for rapamycin's longevity effect, we analyzed the transcriptome of liver from 25-month-old male and female mice fed rapamycin starting at 4 months of age. Few changes (<300 transcripts) were observed in transcriptome of rapamycin-fed males; however, a large number of transcripts (>4,500) changed significantly in females. Using multidimensional scaling and heatmap analyses, the male mice fed rapamycin were found to segregate into two groups: one group that is almost identical to control males (Rapa-1) and a second group (Rapa-2) that shows a change in gene expression (>4,000 transcripts) with more than 60% of the genes shared with female mice fed Rapa. Using ingenuity pathway analysis, 13 pathways were significantly altered in both Rapa-2 males and rapamycin-fed females with mitochondrial function as the most significantly changed pathway. Our findings show that rapamycin has a major effect on the transcriptome and point to several pathways that would likely impact the longevity.” They could do this analysis at a variety of tissues, including the gut, which I perceive I read secretes and might process a lot of physiochemicals

liposomes http://www.ijper.org/sites/default/files/IJPER_45_4_13.pdf

liposomes might or might not benefit rapamycin, a way to tell is if rapamycin has nootropic characteristics, possibly at a higher sample dose, and then see if a liposomal version causes more nootropic effect, notably though liposomes might cause longer plasma half life and could possibly have different peak plasma concentrations so that might effect a nootropic effect

about 76% of some actual rapamycin liposomes get turned into liposomes, with the rest at the remaining fluid, that suggests drinking the extra fluid, or eating the trehalose it might be dried out on could provide another simultaneous, possibly different tissue specific activity of rapamycin

depending on if phosphatidylcholine or phosphatidyl serine turns into, or is highly similar to phosphatidic acid liposomes made of these might be nonpreferred, suggesting a different liposomal production method “the efficacy of rapamycin is dependent on the level of phosphatidic acid (PA), which is required for the assembly of both mTORC1 and mTORC2 complexes. Rapamycin interacts with mTOR in a manner that is competitive with PA. Therefore, elevated levels of PA, which is common in cancer cells, increases the level of rapamycin needed to suppress both mTORC1 and mTORC2.”

liposome preservatives and how long liposomes last with refrigeration, “The use of cryoprotectants such as dextrose, sucrose, and trehalose may increase stability from hydrolysis. Also, samples may experience oxidation upon storage. The addition of small amounts of antioxidants during processing may stabilize the suspension and limit oxidation of the product. SUV should be stored above their transition temperature for no longer than ~24 hours. LUV may be store for a longer period of time if stored at 4-8°C when not in use. Hydrolysis of the lipid begins to occur immediately resulting in monoacyl derivatives (Lyso lipids) which act as detergents and disrupt the membrane, thus permeabilizing the membrane. After ~5-7 days at 4-8°C the internal contents will begin to leak indicating hydrolytic degradation of the lipid. If membrane structure is not a critical parameter in your experiments, vesicles may be stored for 1-2 months with minimal (<10%) hydrolytic degradation.”

distilled water is likely a way to make liposomes be longer lasting, “or by binding metal ions that initially induced aggregation. However, the presence of aggregation can accelerate the process of coalescence of liposomes”

ions at a solution could cause liposomes to last less long, that suggests when making liposomes drug and lecithin, or purified phosphatidylcholine, amounts at something like ultrasonication to make the most liposomes per volume, it is also possible filtering or centrifuging (?) liposomes could divide it from a fluid which might have uncombined, particularly oxidizable drug or phosphatidyl choline molecules

If there are longer versions of phosphatidylcholine they might make more durable liposomes, “Permeability and stability of liposomes are influenced by the rigidity/stiffness of the lipid bilayer.”

liposome size depends on how they are made, ultrasonication produced liposomes make “small unilamellar vesicles (SUV), 25 to 100 nm in size that consists of a single lipid bilayer.” shaking a liposome making solution with your arms makes “Large unilamellar vesicles (LUV), 100 to 400 nm in size that consists of a single lipid bilayer. Multilamellar vesicles (MLV), 200 nm to several microns that consist of two or more concentric bilayers.” it is possible a combination of all three types reaches a wider variety of tissues and likely has greater physiological activity and physiological availability than rapamycin at an enteric capsule

“liposomes prepared by using combinations of some lipids follows the order of physical stability form the correlation of the mean volume diameter, zeta potential and pH , egg lecithin (PC)/cholesterol (CH)/stearylamine (SA) < PC/CH/phosphatidylserine (PS) < bovine brain ceramides (CM)/CH/palmitic acid (PA)/CS < PC/CH/cholesteryl sulphate (CS) at 4°C, as well as at 25°C, 2122after a 6-month storage period” also, “Vesicles composed of saturated phospholipids were found more stable compared to phosphatidyl-choline(PC) liposomes”

Ethanol makes for better drug loading, “that rely on incubation temperatures above the phase transition temperature (Tc) of the bulk phospholipid to promote drug loading. In the absence of cholesterol, liposome permeability is enhanced at these temperatures which, in turn, can result in the collapse of the pH gradient and/or unstable loading. Doxorubicin loading studies, for example, indicate that the drug could not be loaded efficiently into cholesterol-free DSPC liposomes. this problem could be circumvented by the addition of ethanol as a permeabilityenhancer.Doxorubicinloadingratesincholesterol-free DSPC liposomes were 6.6-fold higher in the presence of ethanol. In addition, greater than 90% of the added doxorubicin was encapsulated within 2 h at 37 °C, an efficiency that was 2.3-fold greater than that observed in the absence of ethanol.” also, “Optimal ethanol concentrations ranged from 10% to 15% (v/v) and these concentrations did not significantly affect liposome size, retention.”

Dissolve in etoh first, then put the water in perhaps, perhaps some solvent that rapamycin is more soluble in than water could be put in the ultrasonic liposome maker at a 1 part per 100 amount to heighten colubility, another way is to see if the pile of rapamycin dissolves whn you stir the liposome making fluid

Liposomes can be dried over sorbitol, “formulation of proliposomes lipid dried over a fine particulate, water soluble support like sodium chloride, sorbitol or polysaccharides imparts adequate physical and chemical stability and are ideally suitable for lipophilic drugs” That might be functional with trehalose as well to absorb nonreacted fluids, while the trehalose might also preserve the liposomes

Trehalose as a liposome preservative, at lyophilized of liposomes, “aggregation of liposomes could be prevented by the formation of stable boundaries between the vesicles. The ability of cryoprotectants to form these stable boundaries has been attributed to their ability to replace the bound water around the bilayer via interaction with the polar region of the lipid 36head group (water replacement hypothesis).” also, as to things like trehalose, “high concentrations (up to 30 mol.% in some cases) are required” (to where it is gooey)

the trehalose, possibly, could be added to the fluid ahead of ultrasonication, “In the preferred embodiment, the liposomes are made in the presence of the combination of at least one sugar (sucrose, trehalose, lactose, maltose and glucose)”

liposomes could increase plasma half life of rapamycin availability

antioxidants as liposome preservatives, “use of antioxidants like α-tochopherols, betahydroxy toluene (BHT)”

Rapamycin is made from fungi, “Rapamycin and its analogs are clinically important macrolide compounds produced by Streptomyces hygroscopicus” so depending on how much rapamycin the fungi make those genes could be transferred to plants or perhaps mammals like humans

Rapamycin might last awhile at the body, there are three plasma half lifes I have read, .9 hours, 9 hours, and 56 hours, one thing online says, “The long elimination half-life of sirolimus necessitates a loading dose but allows once daily administration, which is more convenient”

Longevity technology: “Centenarians delay age-related methylation changes, and they can pass this methylation preservation ability on to their offspring.” suggests that drugs, or possibly some non-CRISPR CRISPR like thing could duplicate longevity methylation at everybody

a-tocopherol looks kind of like phenylethylamine, perhaps putting a nitrogen on it, and maybe trimming away a couple CH3s that are on the long alkane distal to the cylic (bicyclic) part, as well as putting a halogen atom on it (I do not know if 2 grams, at numerous doses of a-tocopherol is beneficial or nonbeneficial) so that just a few milligrams or even micrograms rather than 350 mg is a fun dose, also halogenation might outcompete a-tocopherol at some valuable body location, it could be a thing though, a stimulant that is perhaps slightly beneficial

IGF1 (Insulin like growth factor) as well as insulin if modified, perhaps with gene therapy or germline genetic modification could produce IGF1 as well as insulin that do sugar response things while being neutral to longevity, perhaps causing less AMPK activation (If that is what they do)

fetal environment as well as parental epigenetics are published as effecting longevity, methylation, ethylation and other epigenetic things could be modified with supplements or drugs during pregnancy to align the person the fetus becomes with highest longevity, wellness, and healthspan; I do not know if more methylation during pregnancy is the thing, or if just specif areas of DNA methylation are the thing, different tissues, at the devloping fetus have different epigentics,

How much food the parents eat makes a difference in progeny wellness, “There is consistent evidence from both mice and rat models that modulating the maternal dietary status such as protein restriction during pregnancy can significantly affect lifespan”, “F1 male mice exposed to maternal undernutrition during prenatal life produced F2 male offspring with impaired glucose tolerance and increased adiposity”, Also, at humans, “F2-generation offspring of women who were exposed to the famine of 1944–1945 in the Netherlands (i.e., Dutch Hunger Winter) throughout the gestational period had 1.8 times more health problems in their adulthood than descendants of non-exposed women [70]. The offspring of the fathers, but not the mothers who suffered from intrauterine undernutrition during the Dutch famine also had a significantly greater weight and body mass index in their adult life than the descendants of parents unexposed to the famine”, I perceive this suggests that at the fetus different amounts of something like methylation (also acetylation, phosphorylation, methylation, phosphorylation, sumoylation, and ubiquitination) could effect longevity, which suggests drug localization of methyl donors could be beneficial, perhaps optimal methylation of the CNS and heart could be a longevity area, “the epigenetic code changes dramatically during the embryonic development of the organism to initiate differential gene expression patterns between various developing tissues. This code consists of chemical modifications of DNA and histone proteins that play a crucial role in packing the DNA by forming nucleosomes.” Acetylation promotes gene expression, perhaps rather than methylation acetylation of longevity genes could cause greater longevity, also, “The dynamic “writing” and “erasing” of histone modifications are conducted by specific enzymes” suggesting that drugs that cause more beneficial enzymes to be produced, gene therapy as well as germline modification, particularly with tissue localization of beneficial amounts of enzymes could be beneficial, this could be a longevity techology; what one paper describes as socioeconomic adversity “In sons and grandsons of men born outside wedlock, a 1.64- and 1.83-fold excess risk of circulatory disease” Homesis also has an effect, so a drug or supplement that cause the right amount of stress, without the parents feeling the stress could be beneficial, “there is also evidence that exposure to mild stressors in early development can result in beneficial (hormetic) effects and that adaptive modulation of epigenetic processes could significantly contribute to these effects [92–94]. There is also evidence that adaptive/hormetic effects can persist over several generations”, as a longevity wellness drug, it is possible that parents could make their epigenetics (methylation, acetylation phosphorylation, others) like those of persons at the 99.999th percentile of longevity,

marmosets, a primate, could be used to measure the effect of epigenetics on longevity



finding longevity genes other than those that effect mTOR and AMPK: they could look at all the rodents (mice, beavers), and all the bats, and find the ones with the least and most favorable mTOR and AMPK genetics, then they could find the longest living rodents and bats with the least beneficial mTOR and AMPK genetics, it is possible that noting 33 year rodent lifespan difference the long lived non beneficial mTOR and AMPK rodents can be compared with the lifespan of rodents with highly favorable mTOR and AMPK genetics but lifespans of 1/2 to 1/4 or even 1/11 of that, then they can find the genes that cause the greater longevity even though mTOR and AMPK are least beneficial at the much longer lived rodents, also, they can just compare mice genetically modified to have highly beneficial mTOR and AMPK with the genes of beavers to find the beaver non-mTOR and non-AMPK genes that cause the greater longevity even though the beavers might have median mTOR and AMPK genetics; they can do the same thing with bats; The longevity drug benefit is that on finding the longevity genes of rodents that outlive rodents with optimal mTOR and AMPK genetics 4 to 11 times then the products of those genes, and the actual genes, can then be placed at mice to find out if they are longevity drugs and genes, characterizing humans as to the amount of the new longevity genes activity and the amounts of their gene products, is also beneficial.

crispr rodent gene swap, find the longevity gene swapping beaver and mouse genes with CRISPR/cas9 to find out which non mTOR and AMPK genes cause mice to live much longer like the 35 year beavers

Longevity technology:

[Treon Verdery]

Do enzymes make or regenerate NMN at the body? Genes for these could have SNPs that are high wellness phenotypes, gene therapy could be a wellness drug. Although NMN only made mice live 5% longer further study might find a longevity effect, notably from cardiowellnes and perhaps youthful liver function at removing xenobiotics and reducing cancer,

Are there naturally occuring body produced chemicals that contributed to more than half of 20th century cancers? (with the idea that xenobiotics, stress, possibly EM, sedentariness and food might have caused much of the rest) SNPs about these body occuring carcinogenic chemicals could be anti-cancer gene variations that people could optimize with genetic engineering and gene therapy. Insulin and growth factor genetics are possibilities, as is possibly sex hormone levels and SHBG (plasma fraction that gloms steroids); I think there are published systems approaches as well, like mRNA math distribution change with physiological challenges, epigenenetics, and mitochondrial number change.

Longevity chemical: It is my perception that some cytes have less than a dozen mitochondria, and other sites might have near 400; If you do gene therapy or modify the human genome to have mRNA for proteins that would otherwise be affected as to their amount from having fewer mitochondria per cyte from chronological duration effects produced at those cytes (and tissues) that have upper third of mitochondrial number quantity does more of the previously diminishing protein get produced, thus benefitting the body and causing youthful levels of protein to exist? Some proteins travel from cyte to cyte as neighbors as well as circulate at the circulatory system, the proteins that travel outside the cyte could be especially engineering effective at sharing benefit from the gene-therapied, many-mitochondiad cytes and tissues.

Genetic engineering biochemicals to be mRNA coded or produced at the ADP makes ATP cycle, which processes 90 lbs of ADP every 24 hours looks like higher volume production.

I think the ethics and engineering of making humanly intended (mind) physical or possibly neural activation alone, actions modify genetic expression and thus phenotype support the beneficial use of this technology. Prior to 2019 AD and I think anytime, It would have been nice and beneficial, and not that much effort if people had the ability to modify the output of their genes and voluntarily modify their phenotype from simple purposed actions. Things like skipping a day of food, drinking a gallon of water, or recieving a two hour pleasant massage could all be adressable gene switches to turn on beneficial effects while being just slightly unusual enough to omit unintentional activation. Would you like your beautiful personally appreciated hair to grow twice as fast? Get a two hour massage once every 6 months. Getting a massage, even massage at a specific body part to get a specific gene activating phenotypic effect is a possible way to adjust pehnotype voluntarily at the individual, without medical, chemical, social, or fiscally-linked activity based actions. You can give yourself a massage, become more socially fluent and have more fun. It is highly valuable to have previously heightened cognitive ability at all persons, people, humans to higher amount, I think genetically engineering persons, humans, that is people at the germline and thus phenotype to be three, four, or five times more intelligent than I am, with the persons, people, that is humans having the ability to make their beings more intelligent from that is beneficial and more optimal.

Another possibility is a minimal amount of exercise, possibly with different muscles switching on or off different genes, like if you prefer more responsive dopamine receptors (more fun!) then walk more. This increases the amount of mitochondria, the protein production of the cytes, and possibly the actual number of muscle cytes as well. Technologically among the things are the gene therapy or modified human genome concentrating the production of biochemicals or also proteins that can circulate, or possibly things like neuron-localized nerve growth factors produced at muscle that then increase growth of doapmine neurons. (the thighs, but only the thighs produce a chemical, that when it circulates increases a growth factor like NGF or BDNF at just dopamine neurons) So walking exercises the thighs, and that produces chemicals that cause the brain’s phenotype to improve to have more dopamine responsiveness and more dopamine. If you prefer less dopamine you could do sit-ups. wlaking is easier than sit-ups, to my perception, so people with this intentionally changeable phenotype genetic form might sort of have an automatic happiness fun physiological tenedency,although just genetically engineering people and germlines to be much happier is beneficial and I support Dave Pearce’ hedonistic Imperative.


As an early 21st century longevity technology, producing more mitochondria, whether with chemicals, gene therapy, germline modification, or exercise, might increase the production of other proteins at the cytes from simply making more mitochondria, which makes more ATP available, at all of the proteins coded at the genome. It is imaginable that just light exercise could up cytolocalized biochemicals and circulating biochemicals 20%. During 2019 AD many people found it easy and enjoyable to actually double the mass of some of their muscles, so that might double the amount of a preferred protein or circulating biochemical. Different genome product directors and switches than exercise are likely to be more beneficial and popular than exercise. Drinking a gallon of water all at once, or getting a pleasurable massage once every six months is much less effort to do, and sustain, as a goal-seeking activity at consciousness than sustained exercise.

As a technology it is possible to do gene therapy on a just one body part, and then have growth of that part make more of the phenotypic chemical like a protein or other biochemical or chemical, to produce local effects (GFP output changes with tissue use which I think is published as increasing transcription at the utilized tissue, exercising should therefore make GFP at muscle tissue increase, and instead of GFP it could be different physiologically beneficial protein or other biochemical)

Surpisingly muscle, which coalesces to multinucleus cytes and can have its number of mitochondria heighten just with use, could be a location to produce beneficial proteins with go-everywhere-activity, although other gene directors and activators are likely to be more avidly used, like massage, skipping or eating an anomalous amount of food, or getting a massage, even massage at a specific body part to get a specific phenotypic effect.

(I may have read that 80 year olds might have only 40% of the mitochondrial numbers as 20 somethings); this affects how much energy there is to produce new proteins, how much is produced, and possibly if they are produced at all. I think I also read that the number of mitochondria, which goes with the amount of )

I read ATP might travel on actin filaments, somehow, even though ATP is an eentsy molecule. It is plausible that tubulin also effects ATP transport. If actin or also tubulin transport of ATP is actual, then drugs or genes that modify the ability of actin to transport ATP could be wellness drugs and possibly longevity drugs, or even treatment of illness drugs. Also as a possible wellness longevity drug or genetics is the effects on the actual location arrangement and network of the actin or also tubulin that transports the ATP. Multilane highways or dendritic branches? Concentrated depots and paths, or partially populated walkways? There is a possibility that a computer science approach to optimizing the mathematical network form of actin or also tubulin transport of ATP could benefit humans at the cytological level with drugs or genes that reprogram actin or also tubulin based transport to optimize human well being. I perceive I have heard about research on what actin and tubulin goes where during meiosis and why, so perhaps some of those techniques and research could benefit the technologization of actin and tubulin engineering for wellness and longevity.

I read about some chemical in Scientific american that is sometimes described as “exercise in a pill”; exercise increases the number of mitochondria at muscles, perhaps “exercise in a pill” at the circulatory system reaches more cyte types, even neurons and also tissue types and increases the number of mitochondria at them, increasing wellnes and possibly heightening lifespan from removing nonoptimal effects at linked dynamic systems.

Another thing that increases the number of mitochondria is branched chain amino acids, “Branched-chain amino acid (BCAA) supplementation increased mitochondrial biogenesis in skeletal muscle [4]. BCAAs are 3 of the 9 essential amino acids required for humans.” [not made at humans though]

Genetically engineering food crops to make BCAAs could have quantitative measurable wellness effects, and just possibly from more mitochondria, beneficial longevity effects. Soybeans, wheat, legumes all contribute to protein sources during 2019 AD, and these nd other plants could be engineered to make BCAA.

Developing a plant-based milk that tastes more delicious to the majority of humans than dairy milk would be a valuable ethical vegetarian technology. Among Europeans and Americans milk, which could be technologically improved to be better-than-dairy-milk plant based milk, provides much protein to many people. Genetically engineering plant based milk to have BCAA, possibly BCAA optimized to generate more mitochnodria is beneficial.

Technology that enhances vegetarian milk: Are there any good tasting surfactants? that would make the little globs customizable in size for flavor adjustment (improvement).

Can you put veggie milkpowder as a thin layer through an embosser with the resolution, or higher, of a holographic decalmaker? Microfeatures at blobs eentsier than a wave of light could then be used to technologically improve the milk product as solvated globs; Also I have read about superhydrophilic and superhydrophobic surfaces, teeny spires (hydrophobic) or angled sided spaces that look like ingots (hydrophilic), it could be that embossing vegetarian milk powder to make hydrophilic or hydrophobic features could enhance flavor. Also there is customizing globs to physical tongue receptor size for an optimal fit. Uncomplex embossing of a powder could be ultra-fiscally favored. Ihave seen candies made of sugar that have a hologram embossed on them, so lightwave feature size at carbohydrates is a technology that has existed since the 20th century.

.5B GSK:
I perceive that GSK may have (or may not) have links to companies like nestle; could cocoa, and possibly thus chocolate, be wavelength of light feature size embossed to enhance flavor?

Are there any naturally occuring amino acids or peptides that activate the taste receptors that activate when milk tastes good; this is completely different than MSG (monosodium glutamate) in milk, but the technology of an amino acid or eentsy peptide that enhances the flavor of veggie milk could be possible.

school lunch veggie milk
It seems like things like vegetarian nut or soy milk could be cheaper to produce than dairy milk. I think it is possible to develop the vegetrarian milk flavor until it is preferred over diry milk among most people. It is possible that more people, throughout their lives, would like an utilize vegetarian milks if they received them at school lunches.
It might even be possible to use vegetarian milk to cause people to be weller in different ways. If the serving size of vegetarian milk is 10 Oz, as compared with the previous 8 Oz at dairy milk at identical calories, then people might drink more fluid at school lunch. Drinking more fluid at school lunch causes a population effect of eating fewer calories at the rest of the food. Then noting that at 2019 AD, research supported that vegetarians had less heart disease, cancer, diabetes, and weighed less with a better BMI than the rest of the population, the school administrators could say, “there’s nothing wrong with our food, but it’s awesome students are drinking nut/soy/oat milk and eating less of the other items and more vegetable sorced food instead” So the people at school get more ounces of milk, they are, as a population at least slightly wellness heightened, and the school district spends less on the milk. That all combines to support vegetarian milks at school lunches.

MWI, that is Many Worlds Interpretation of physics: thinking of ways to cause a larger number of MWI universes to be generated from something going right, that a person, that is a human likes: The amount of quantum states produced from a proton (protons have their own quantum effects, among them tunneling) is, I think, larger than that of af a neutron

Note: about protons or neutrons having different numbers of quantum states, and that they each generate quantum state changes at different amount for each unit of chronological moments: I think protons have more quantum states as an area with t as a dimension, although it could be otherwise. Thinking about the quantum effects of neutrons ( I am thinking about neutrons taht are a part of atoms): if a neutron (just one as part of an entire atom) is changing a chemical rection in a flask with the isotope effect it may or may not be having a quantum mechanical effect at a multitrillion atoms simultaneously at chemical system that is some chemicals in some water in a flask.

That the isotope effect of one (part of an atom thus nondecaying) neutron, which should have at least some analog effect on all the actual atoms at the chemical sample if analog, functions at a distance (at an aqueous chemical system it could be nm, Cm or Km); the analog possibility comes from what seems like an analog nature of things like circles and various math of topology that seems like they could be having an effect at a 3d chemical system; The thing is that I perceive a physicist might say, “even though you can observe it into a circle, or a sphere, or even that the sides of the container are changing (however minutely) to produce either space or time analog-like things, it is all describeable with a quantum wave function and quantum equation, so prior to 2019 AD observations that support quantum theory support that it is actually just a quantized system, the neutrons are not having an analog effect when one neutron produces an isotope effect on an entire flask with quadrillions of atoms at a flask of aqueous chemicals”.

That a neutron being turned into a proton causes the number of quantum states possible, as well as generateable, per unit of chonologically measured moments, suggests that making neutrons into protons could be a technologizable way to purposefully make a larger number of MWI universes when a sentience, like a person, that is a human likes what is going on.

When a neutron is turned into a proton, that proton often attracts an electron, (does the casimir effect preclude the possibility of naked protons staying solitary?) which then do electron and photon quantum things, numerous of which are described at peer reviewed published material as being quantum effect actions, each quantum event with an MWI branch universe 92019 AD theory); converting a neutron to a proton then causes a much larger number of MWI branches to be generated, for the theoretical duration of the universe. Although I am not yet educated sufficiently able to use aleph numbers to do calculations, if the universe happens to be chronologiclly nonfinite then the number of MWI universes generated from one neutron being converted to one proton is a nonfinite number, and further, that nonfinite number has many many MWI branches (I perceive I have read that human researchers might have different scientifically supported ideas about whether MWI branches are finite or nonfinite in quantity).

Thinking about a new proton coming from a neutron: a new proton at a chronologically nonterminating universe would have a nonfinite number of MWI branches though; or perhaps could, I read about people who think cooling all atoms to their quantum ground state is a spontaneous actual process at the universe; although MWI suggests that things like time crystals and the delayed quantum choice eraser could effect quantum-capability of atoms retrocausally or cyclically at some amount of the MWI universes; I have heard of these things, so the MWI universe I am in might be entropically nonmonolithic

A technology that utilizes the difference between a proton and a neutron to cause more MWI branches where a human things things are pleasantly going the way they like. I like Serina, so when I send her a text, and she replies I think my life is going well and I am particularly enthused about MWI branches arising from a universe where Serina texts me, I then press “make proton” on my phone, and the phone makes some protons out of neutrons. That generates a nonfinite (proton at nonchronologically finite universe version) number of universes that branch from one where Serina communicates with me. I could even press on “make lots of protons” if Serina says something like, “I like you” or “let’s meet”.

AI and software developing MWI technologies: With MWI technologies it is my perception it is endless quadrillion of times better to write them immediately rather than wait a moment to write them as a vast plurality of MWI branch universes will potentially benefit from new, accurate, actual, technology producing MWI content; that suggests that AI (artificial intelligence) writing, communicating, or doing experiments could then validate and quantify the “research on the MWI should be accomplished immediately as it affects endless quadrillions of universes differently if you delay 1 millisecond” way of looking at the MWI. A human that builds an AI that figures out new things about the MWI, or even just develops the math and technology space, a few trillion times faster than a thinking writing human is a thing that could be of value from the perspective of a person like me that values making happier more optimal, Dave Pearce’ headonistic imperative actualized universes. (AI produces MWI research and technology a trillion times faster than me) During 2019 a 4Ghz processor would do 4 billion computations a second, and 250 seconds would be a trillion computations, so an AI doing a few trillion more MWI research things and technology items than I would in an hour is plausible; a few sequential instructions at a parallel 4Ghz AI compared with a few minutes of human thought.

Immunizing against a million topologies; screen antibodies to things like “a torus of alpha helices” or a “square of beta sheets”, or “something made of protein twice as wide as it is long” at human tissue culture. This edits the amount and kind of chemicals and biochemicals shared between cytes. Then find out what these categories, and mathematically developable forms (if it kind of works on one topology, then they can find logical math extensions from that topological shape; immunizing against alpha helix torus is 20% effective at heightening organism, like a human, that is a person’s longevity, so the math suggests: vary diameters, immunize against an “8” and an “88”, and immunize against a tetrahedron made with a torus on each side, and others) of them to find the topological antibodies that cause human tissue culture arrays to be weller and have greater longevity; (immunize against many to mid AMU rod looking groups, curlies, heaps of alpha helices, piles of beta sheets)

Immunizing that prevents senescence could be the effect of screening topological libraries of antibodies. Notably research on senolytics apparently supports this. Senolytics terminate senescent cytes; as a result the senescent cytes cease to export chemicals and biochemicals to their between-cyte environment as well as the circulatory system. Those senescent cyte produced chemicals caused unwellness at other previously well and non senescent cytes. Terminating the senescent cytes causes the well cytes to have things continue to go well, and I think I read that mice that get senolytic therapy are healthier, perhaps phenotypically younger, and have longer lifespans, all from eliminating the chemicals the senescent cytes made. So, could you immunize a lab mammal and then a human, that is a person, against the entire library of chemicals and biochemicals that the senescent cytes produce; such an immunization could have the effect of a senolytic drug as it mops up the nonoptimal chemicals.

So, supporting the screening of topological antibodies, senolytics’ effectiveness and conceptual form apparently supports the idea that there is a whole bunch of things, and that if you treat them as a group, you can get an overall bulk effect, even though the bulk effect could contain optimal and nonoptimal chemical effects simultaneously.

Some different bulk effects at the human body that I think are published as causing benefit are things kind of like: more mitochondria causes more protein production, and more mitochondria thus produce greater wellness and youthfulness. Another bulk effect is putting more genes, of any kind, on histones, I think (possibly) causes greater longevity and fidelity of proteins produced.

As a bulk effect, immunizing against topologies could produce beneficial effects like wellness and longevity at the entire body. The thing is to make a lot of them and screen the library to find the ones where the average and distribution of the blended bulk effect is quantitatively and qualitatively measured as much better for the human than an absence of the topological immunization.

Going with this technology of Immunizing against extensible classifications (like topology) could be immunizing against electric charge of an entire protein. Immunize against everything with -.499 to -5.00 charge and a thousand others from +4.99 to -4.99). this diminishes the amount of the proteins circulating, or zapping those cytes with these charges of protein at their surface; this would have a bulk effect, and then the bulk effects of a few thousand variations, or a few million variations, are listed at a computer with the immunization that produces the greatest beneficial bulk effect at the top of the list.

Screening topological and protein molecular electronegativity: Make a human tissue culture of neuron, cardiac and other human cytes, although multiwell plates already exist, you could use IC production technology to make a 1000 times 1000 grid, which is one million human tissue culture samples per screenable plate. The thing is that if you screen a 1000 times 1000 times physical array you have characterized the effect of more than a million different antibodies on the wellness and longevity of things like neuron and cardiac and other tissue culture, which could be predictive of wellness and longevity effects at those tissues and also the entire organism.

Notably increasing wellness and longevity at the entire organism is the resulting beneficial drug from screening vaccines to topologies at a big array. These new wellness longevity drugs are produced when topologies and electron negativities of proteins are found that have beneficial bulk of physiology effects.

I read that BCAA cause more mitochondria to be at cytes. A person with abilities at planning and creating food, might be able to make a new popular food based completely around BCAAs, possibly a completely new BCAA, with a different amino acid sequence that tastes better than 2019 A.D. BCAA (Noting the mitochondrial amount going up I think I read, has higher wellness (and possibly longevity) effects.

I perceive that caffeine is a 2019 AD popular drug, notably at beverages.

So, can you attach a biguanide (metformin-like molecule) to caffeine, which alread tastes aversive, to make a longevity version of caffeine that is a stimulant that makes people live longer and have less heart disease and less cancer. Lilacs make biguanides, tea makes caffeine, so both at one gene engineered plant could be produced. Along with the rest of the world China might go for longevity tea from a GMO crop.

If you chelate beneficial things, even like a biguanide (metformin-like effects) does the taste become much milder? EDTA seems to be mild flavor. Are there any naturally occruing plant products that are chelation agents? Then you could blenderize and fractionate the source plant and soak the thing to be chelated in the soultion that had the naturally occuring chelator concentrated.


Although ECGC is already at tea, perhaps it could be engineered into coffee.

Curcurmin comes from a plant in the ginger family, perhaps ginger could be engineered to be good for people and make curcurmin. A mild flavored more massive Ginger root with curcurmin might replace the potato

Tea bred or engineered to have 10x as much ECGC, noting tea already produces ECGC.