Longevity technology

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Treon Verdery

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Sep 30, 2022, 8:56:12 AMSep 30
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Peelies from sun exposure relief technology: 10hda is published as reducing scratching multiple times; screen a decanoic ester library, that finds a molecule that does better than the multiple times less scratching that nude mice do when they have dermatitis and have royal jelly topically applied to them while being at greater potency per mg or microgram, Put some kind of dermis passing thing on or with it, beauty emulsions say they penetrate the dermis to bring active ingredients to the dermis so those technologies can bring decanoic acid esters to the living part of the dermis

so it can soak through the peelie and get to the itchy live dermis under the peelie. I have also read multiple pubmed journal references comparing topical dermis youthifiers and the references say some of them are highly functional so dermal transport media or moieties are to my perception functional

Oral version

Topically, harmless antibodies linked to fluoroethynyl phenibut like chemical, could possibly concentrate at the dermis.

An oral version with enteric coated liposomes to deliver the protein conjugate drug could take numerous minutes to reach the small intestine but make people feel better for many hours and as a pill is more likely, I perceive, to be utilized.

The possibility of a fluoroethynyl peptide or a harmless antibody linked peptide suggests less than 100 micrograms would dose the entire volume of a 70kg body, so a few kg of dermis is likely less than 11 micrograms per application, at $435/g (retail antibodies) that is near 43.5 cents a milligram or near .0435 cents per topical dose or .29 of a cent per oral dose

Is there such a thing as well feeling and appearing dermis, itchless and zero hue change skin puffiness causing chemical; one possibility that is similar but kind of different is topical vasodilator, possibly niacin, which i perceive i may have read about as a topical beauty treatment; if there is then doubling fluid flow at the capillary epithelia could make a topical peelie comfort medicine absorb twice as rapidly or ultra hydrate the dermis causing more rapid diffusion and greater activity of the topical drug causing more rapid function; I have heard of vasoactive peptides, perhaps they could function;

A zero hue change completely comfortable dermal vasodilator might also double the effectiveness of things like peptide beauty topicals, it makes me think though, they could just double the amount of active ingredient;

Find out if topical or oral phenibut, possibly a halogenated phenibut, or GABA active peptide effects dermal nerves to cause the removal of itching that about 2 grams of oral phenibut causes. A body side of the blood brain barrier possibly halogenated phenibut or other body side of the blood brain barrier GABA active molecule could omit CNS GABA activities, it is beneficial to have the drug be absent effects on things like driving ability, reaction time, or social skills.

Thick throat coating drink with peptides might have sufficient surface area to be absorbed as a kind of oral-like peptide dosing form, although I have heard of peptide doses up to 1 mg my perception is that at things like vasopressin and oxytocin it is much less, the most dose effective peptide I have read about, having not looked up other things is a mu opioid peptide active at nanogram amounts, I perceive I read about a halogenated peptide that was twice as effective; at an oral like mouth and throat coating peptide dosing form it is possible the pH of throat saliva could be modified and a mucolytic thing, perhaps something like a protease that is absent an effect on the peptide could make the peptide actually contact the dermis at the throat and mouth, the thing is though, what about stirring? A mm layer of colorant will just sit at a glass, for I perceive, hours, before diffusing; effervescence could do it, so perhaps a bubbly mini-beverage could make the body surface dermis soothing peptide reach the throat mucosa dermis many times more rapidly and before it was washed to the stomach; beneficially the peptide would only work at the mouth and throat so if many doses were consumed because the bubbly mini-beverage tastes good the dose would not vary that much

The 1/10 of a cent printed paper dose technology with the 1 cm^2 doses and enteric layered ink could be beneficial at the developing world, the thing is, what drug, like antibody linked GABA active peptide or drug, would soothe the dermis; tetracycline and possibly minocycline do build up at the dermis, possibly a GABA active drug linked to one of those molecules that has been screened to be a neutral biotic, possibly also finding a variant with greater dermal localization

An all natural product could also function, I do not know of any anaesthetic macroscopic or other fungi, but if there are any, notably GABA receptor active fungi chemicals, then those could be a rapid product.

Actually I am not aware of any natural product that effects GABA receptors, there are commercial natural products chemical libraries that exist, they could screen those to find out if any of the natural products effect GABA receptors, GABA the chemical does not pass the blood brain barrier but might diffuse through the dermis and is only a few $ per 100g.

Other neurotransmitters at the dermis could be anesthetizing, heightening response of anadamide receptors is unknown, a mu-opiate peptide that disintegrates in saliva, mucous of any orifice, and the stomach could be a topical anesthetic, perhaps a mu opiate peptide with a magnesium or calcium or zirconium or scandium atom in the middle of it could persist long enough at the dermis, or lasting about 90 seconds, but continually diffusing from the topical material would function

Orally a mu opioiod peptide attached to a harmless dermal

Localizer molecule, possibly an antibody or a minocycline variant could function. As a topical drug the peptide could be modified so that many enzymes degrade it so it would be nonseperable from the localization chemical which might also be made of enzymatically degradable nucleotides, peptides or proteins

Things that soothe UV Elie's could ask soothe dermatitis as well as a topic dermatitis

Theoretically about7.6 billion people on earth have experienced sun based peelies, so this could benefit people

I have not read about brain area localization technologies applied to mental illness drugs but it seems possible it happens, area (like neocortex) is a different thing than neurochemistry, like say all the neurons with AMPA receptors, or at antischizophrenia medication 5HT2 receptors at pimavanserin,

They could screen a library of fungal chemicals, noting fungi have numerous unusual chemicals after utilizing a radiolabelled acetylating chemical to acetylate a bulk fungal extract, so the chemicals pass the blood brain barrier they could separate the chemicals (more on that at a different note) and feed them to mice, one new chemical each 24 hours, and then brain scan them, and with 300 mice a month later would have 9000 localization maps; if 1 out of 100 had utility they would have 90 maps, also they get maps of where things localize at the body area as well; then noting what the concentration areas are they attach drugs to that fungal molecule and see if the molecules still go to the mapped area; they could come up with molecular variations on the fungal molecules that were absent any drug effect so the drug molecules the fungal molecules were transporting did all the pharmacological activity, it is possible that enzymatically degradable linker molecules could separate the active drug from the fungal transporter. They might do things like this now I do not know.







Separating chemicals from each other with microfluidics,

Another possible way is ion exchange resin inkjet printed beads, i think i have heard of printed microfluicics and i have heard of 3D printed microfluidics, it might be possible to inkjet print digital gradients of ion exchange resin component chemicals, then aim a warming laser to melt them so they bead up, then high frequency nonwarming pulse interval laser to stir them, making a digital gradient of different ion exchange resins responsive to a different chemical; a microfluidic channel layer is then printed atop this (or previously beneath) and each of the separate ion exchange chemical concentrators has its own plumbing, at 32 micrometers radius of the bead then there are 47,851 chemically different chemical separation locations on a 7 cm times 7 cm chip

and possibly

or if 3D printing are printed on peltier cooled paper; or 72°F stable then they melt, liberating contents when a laser shines on them

Another way to make an IER chip is ion exchange resin inkjet printed beads, i think i have heard of printed microfluidics and i have heard of 3D printed microfluidics. It might be possible to inkjet print digital gradients of ion exchange resin component chemicals, then aim a warming laser to melt them so they bead up, then high frequency nonwarming pulse interval laser to stir them, making a digital gradient of different ion exchange resin beads responsive to a different chemical; a microfluidic channel layer is then printed atop this (or previously beneath) and each of the separate ion exchange chemical concentrators has its own plumbing, at 32 micrometers radius of the bead then there are 47,851 chemically different ion exchange resin chemical separation locations on a 7 cm times 7 cm chip

Another way to make an ion exchange resin chip is

If you liquefy an ion exchange polymer with a laser or ambient warmth then use the actual multichannel microfluidics to transport the ion exchange polymer fluid to a particular well. It is it possible to fill 1 million different wells each with a different chemical component ion exchange resin; blending liquid polymers, using microfluidics, at a variety of ion exchange resin chemical components that are blended together to make an array of 10,000 times 10,000 ingredient gradientized different ion exchange resins, each of which will prefer to glom different chemicals than the other, doing that makes 100 million different ion exchange resins at a chip

It also makes me think this is a possible way to do what is effectively screening a library to find completely new ion exchange resin chemistries, if you then pump in the chemical you want to glom on an industrial scale, and have the microfluidic then sequentially (parallel versions could work) liberate the glommed material you could find the most effective ion exchange resin composition out of 100 million (wells) which is likely much more efficient.

Niftily, ion exchange resins tuned to find chemicals of moderate electric charge and higher lipophilicity tend to concentrate the 100 million chemicals most likely to pass the blood brain barrier and omit macrophage and leucocyte response; that kind of ion exchange resin chip could enrich the number of chemicals concentrated from the homogenate with chemicals that have more favorable drug characteristics which are then higher velocity at being turned into drugs

It could be possible to heighten ion exchange resting chemical concentration 11 times greater than wells with ion exchange resin fluid traversing sponge:

ion exchange resin highly porous sponge, blending saliva with rubber cement automatically makes a sponge so a chemistry of sponge making at microfluidic tube chambers could be straightforward, it is possible that rather than an ion exchange resin bead well you would just make a higher volume tube volume of like a (========) filled with ion exchange resin sponge along the access plumbing microfluidic paths; think of a view like a circuit board multiparallelism with tube balloons periodically, the tube balloons full of ion exchange resin sponge could have surface areas to the 3rd power causing them to glom with 32767 (32^3 micrometers) compared with (32^2 times 3 micrometers) at a well, that is near 11 times more material sorted at a similar surface area but sponge hosting volume

If ion exchange resins can glom 1-10 pct of their volume of product, and 47,851

1/32,000 of a milliliter is the volume ofa 1 cm^2 area 32 micrometers deep, if the ion

1 cm cube that is 2/3 tubes concentrates 1-10 pct of chemical so .067 ml of concentrated material and can be used 11 times, so .67 ml of material

Compared with the 27 item multicuboid concentration and sortation reactor which is utilizes 27 cuboids, each 2/3 ion exchange resin sponge tubes, utilizable 11 to 140 timesreactor

Parallelism is beneficial at chemical sortation and chemistry chips, noting the 1 cm^2 two thirds of it being ion exchange sponge filled tubes product sorter and concentrator, When assembling a cuboid (like 27 packages stacked three on a side) microfluidic chemical reactor or ion exchange resin cuboid form, a Lego (or better) interclick of perhaps 16 tubes per Lego circle thing to make a 27 times capacity reactor or product concentrator/sorter goes with having functional fluidic,and microfluidic connection between the cuboids;

the actually fairly mild 16 times 6 circle tube fittings times 27 cuboids times 6 sides all surface Lego circle connectivity reactor would have 15.5k connections, perhaps better than brickle blocks is tHZ (or, just possibly at some polymers lasers like the 3D shape deepwater laser scanner) weldable cube internals, so the up to 10k connections at a 27 cube Lego assemblable reactor (only about 10k connections out of 15.5k if you ignore the surface) could be Thz welded, puffed to fit, or even capped if leaky, at a yield of just 95% 10,000 connector connectiviyy that produces 95% or higher functional plumbing access piping at the reactor multicuboid. The microfluidics at each cube could have some adaptive routing paths to make all the reactor/concentrator well or tubes accessible. I think making these as spheres is also beneficial

The routing adaptive layer at the microfluidics makes it possible at a 27 cuboid reactor to route the chemical containing fluids clean withe reverse direction flow

Ion exchange polymer reloading as microfluidic pumping of new gradual chemical reaction to make new foam, laser or Thz

it is possible a soft flowing polymer with "brickle blocks with adaptive routing" could avoid the actually fairly mild 64 times 27 times 6 all surface Lego circle connectivity, perhaps better than brickle blocks is tHZ weldable cube internals, so the up to 15.5k connections at a 27 cube Lego assemblable reactor could be welded, puffed to fit, or even capped if leaky, producing 95% or higher functional plumbing access piping at the Lego cube. I think making these as spheres is also beneficial



Integrated circuit technology microstructures; doing electrophoresis at microsized channels that are likely larger than the one million lane microfluidic chip I read about; An electrophoresis gel that is only a gel when it is cooled can be partitioned and pumped around when it gently warms, although there is also the possibility of specific area of the chip warming with lasers; the many lane electrophoresis peltier cooled gel turns liquid at 16° then adjacent microfluidic lanes at 32 ° angles pump the chemical or protein enriched fluid at just that area to a new location on the microfluidic chip; 1000 lanes are loaded at one side of each electrophoretic lane, so each electrophoresis lane at the chip can transport 1000 completely different chemicals like proteins

There many ways to structure the microfluidic channels, some of them remind me of the connected and branching conductive lines on the upper surface of photovoltaic

Looks kind of like (→≠») or ==‡== where fluid flow direction meets a bunch of channels connected at an angle or #€[‡] other branched channel geometry

The liquid sample with liquid electrophoretic gel material that gels at 16°C loads at []‡ or ==‡== then peltier cools the multichannel ‡ to gel, then an integrated circuit, which just possibly is at the base of the electrophoresis chip if it is made with integrated circuit technology as compared with polymer technology or MEMS technology, causes voltage at the long (‡)channels on =‡= causing the gel to sort chemicals, this can all be acvomplished with branches of branches at one layer, or, layer atop ==== has another ==‡== fluid path on it; each concentration at the gel channel can be liquified with lasers; microfluidics utilizes published pumps so above 16° C when the === areas when the concentrated chemical are liquid they are micro fluidically motionized to the perimeter of the chip for further use, alternatively they can just be micro fluidically transported to where the yeast are; at another version the microfluidics transport the electrophoresis products to a bunch of wells on a multimillion well integrated circuit technology like planar area, and then to place the yeast and nutrients, the mechanisms automatically, or if feeling thrifty, a human gently places, or possibly even mists the multimillion well plate that has the plurality of separate concentrated chemicals that the microfluidics have placed at predictable locations and at a database, the actual yeast and nutrient solutio; the wells have the volume capacity to feed yeast for 20 unmedicated yeast lifespans noting that yeast now can be made to live 400% longer (salicylic acid) so that way if the 99.999th percentile of longevizatiom causes a lot of yeast longevity they will have nutrients the entire time.

It is possible putting the entire thing on an ultrasonic transducer will heighten fluid diffusibility

To produce a larger amount of chemicals, utilize some other channel sizes, make the multichannel integrated circuit technology electrophoresis chemical concentrator produce 100 MCG of product per each second or third branch (or layer) of sequentially purer electrophoretic ==‡== channels, 1000 or 10,000 ==‡== functioning in parallel, loaded from the sides of chip with larger distributive microfluidic |[]| perimeter channels, provides higher volume of material being concentrated; there are 2500 preconcentration material loader channels on each perimeter side; 100mcg times 10,000 ==‡== channels processes 1000 mg, 1 gram of sample each full cycle, at 20 minutes per cycle then 72 grams of sample can be separated per day, 14 of these chips stacked in parallel can process and purify 1 Kg per 24 hours



If a yeast weighs a microgram, and it is possible there is a yeast species that massive (1 million yeast per gram) then dosing the yeast at the human equivalent of 2 grams to 1 micrograms human equivalent dose with the chemicals concentrated at a microfluidic electrophoresis chip is possible

Notably having millions of wells at an integrated circuit wafer technology or even MEMS polymer form makes the amount of locations so the yeast can be dosed at numerous different orders of magnitude, about 7 orders of magnitude between 2 grams (phenibut) and 600 nanograms (ethynyl estradiol) human equivalent dose at 8 wells of yeast each to get p

What if the microfluidic chemical sorter had 100 micrometer channels, and 100 of them on each perimeter side that are moving preconcentrated material at each perimeter side of the chip, well that perimeter is a surface area kind of like one cm^2 of motionized fluid; Depth: at 100 micrometers of 16°C electrophoretic liquifiable depth, and 11 minutes per cycle to traverse up to .5 cm (before branching) while having 10,000 micro fluidically addressable tube segment tapping branches (noting the 1 million microfluidic lane chip I read about) then it is like a volume of 1/100th of a ml each 11 minutes or about 1.3 ml per 24 hours. A 7 cm^2 version would produce 63.7 ml of 10,000 sorted chemicals. If less specific lengths of the liquified electrophoretic lane tapping tubes were utilized then you could get larger amounts of 1000 chemical fractions, at 63.7 ml that is 63.7 mg of each of the 1000 different chemicals, although it is actually different than that from solvent mass, so 6.3 to 32 mg per 24 hours could be from 90-49% liquigel solvent

The thing being that the person is producing mg or more of a longevity drug to characterize and quantify at 6 month 96 well plate fish or mice,

If 1 out of every 1000 or 10,000 (different chemical quantities from different resolutions that make .63 to 6.3 mg of concentrated chemical to dose organisms with)

That makes 10,000 or 1000 microfluidic branched channel electrophoresis concentrates, of different numbers of simultaneous chemicals from few or even one to ten times as many from ultrasonicated endolith homogenate. These 1000 or 10,000 chemicals could then be screened knowing, kind of, what they are. Also the microfluidic chip could have 10,000 wells on it so an eentsy sample could be removed if really precise characterization is the thing to do. The amounts, going with the convenient unrealistic presumption of 1000 chemicals of equal amounts is sufficient to dose a mouse with a multidays of dose produced every 24 hours, or at 10,000 chemicals, c elegans. The c elegans could be dosed throughout its life from one 24 hour output. This is

A way to make 99.99th percentile of longevizing chemicals at 24 hours. If age batched c elegans were used for testing then 6 or 9 days would produce a longevity increase %.

Finding the longevity drugs:Use microfluidics to connect the multimicrochannel electrophoresis chemical separator to 100 million yeast colony wells or 100 million human tissue colony wells then use the: the more GFP it produces the longer it has lived varieties of yeast or human tissue culture cells, these emit more light the longer they live then have camera note the most light emitting cells at wells,

It is possible that finding a most longevizing chemical from half a billion different chemicals longevity drug chemical could cause a person to systematically follow twig to branch on a half billion leaf treeee, possibly doing all 29 Binary experiments to find the identity of the chemical. exposing yeast to half a billion different chemicals, then utilizing flow cytometry to find the one with the greatest light emission tells that if it turns the yeast into anendolith lifespan longevity organism it is beneficial to test it on mammals and give it to humans;

Utilizing liposomes with, approximately, just one chemical at them to quantify and characterize new longevity drugs:

With the possibility that a continuum gradient electric charge or pH gradient generation around liposomes, causing them to ensconce different things (or putting phosphatidyl serine and alcohol at an electrophoresis gel that liquefies above 16°C then beaming ultrasonics on the phosphatidyl containing gel produces liposomes to form right there, at a micrometer or eentsier sized piece of a chemical/protein band, ensconcing just that location's chemicals at that electrophoresis band generates a billion or a trillion liposomes, each with mostly just one particular chemical;

You blend the one-liposome-one-chemical liposomes with a one yeast one liposome dilution at a container of fluid, or possibly decanted to make a monolayer; longevity chemicals make the yeast live longer and the most fluorescent yeast has lived the longest, the longest lived 700 yeast are detected with 20 million yeast per second flow cytometry and a numeric characterization of that entire library like endoliths or the kings holly can be made. finding something that makes yeast live numerous orders of magnitude longer and noting flow cytometry lets the yeast live on, many of the yeast remain alive

Then there is a way to trace the long lived yeast to the liposome it ate, and the chemical at the liposome; it has to do with the sensitivity of isotope spectroscopy at flow cytometry, the liposomes can have a large location specifying dose of stable isotopes at them because the electrophoresis gel either migrates a gradient of isotopes with 2-6 different isotopes migrated from each distal part or face of the gel before electrophoresising the screenized material like kings holly or endoliths; so the

isotope gradient, deuterium from one distal part, nitrogen from the other distal part, phosphorus from a transverse side and oxygen from another transverse side; each liposome also then gathers a region associated multiisotope ratio at amounts that can be seen with spectrophotometry of the yeast at the flow cytometer if that narrows the content of the liposome to an eentsy area of the electrophoretic gradient distance (microfluidic is micrometers) then the name of the source and its library of congress number is known and then the process is repeated to find the page paragraph the particular chemical molecule is

on.

Microfluidic version, transport numerous nearly identical electrophoretic liposomes sequentially to the yeast upping isotope dosage two or three orders of magnitude, and heightening actual chemical dose.

Liposomes are make able at different volumes, it is possible that liposomes with 300 times more dosing chemical are valued or possibly really eentsy liposomes with higher chemical/spatial resolution are valued.

possible then when a trillion liposomes form there could be a billion liposomes amongst those that had mostly just one kind of chemical or molecule, the one near them on an electrophoretic band that could be ensconced into a liposome at just that minute variation in isoelectric point, pH, p(some other chemical like NH3) (pNH2), so with a trillion gradient formed liposomes you get a billion mostly one chemical liposomes, (even though only a billion of them are highly focused)

Then After dipping, rinsing, liposome surface reacting, the liposomes with something like ribose or something yeast like to eat, put them at a dilution fluid so the ratio of liposomes to yeast is one liposome to one yeast; the yeast eats the liposomes then You can tell if the liposomes chemical longevizes because things like known longevity drug liposomes make their individual yeast live longer, and the liposomes volume and appetizing coating can be adjusted to get the highest ingestion and longevization response

Does this work with daphnia; could you laser zap an isotope gradient preloaded then longevity drug electrophoresisized gel to make eentsy 11 micrometer sized daphnia food cuboids, then feed them to daphnia to characterize and quantify the longevity effects, doing flow cytometry with spectrophotometry to find the isotopes that describe the location on the electrophoresis gel which tells which chemical book I'd or neighborhood it is from; daphnia are possibly a better longevity quantification organism than yeast.

Could isotope labelled electrophoretic liposomes screen billions of potential antibiotics rapidly, the bacteria that eat the liposomes with the most powerfully antibiotic fluotesce the least when all the nonliving bacteria are orocessed at the flow cytometer. new antibiotics save lives

Grow a sprig of the king's holly in isotopically labelled water, or perhaps do gene therapy on it with isotope labelled DNA, or provide it with isotopically labelled amino acids, or do all three with different stable isotopes; then do electrophoresis like microfluidic electrophoresis or bulk liposomal electrophoresis then when the flow cytometer spectrophotometric thing detects isotopes it is detecting them on actual longevity chemicals



It is possible that some drugs or other chemicals have very high area localization at the brain or body as they are, but that nobody knows of any illness linked to that highly reachable mapped area. Based on how it would likely function to attach another molecule, a drug, to the chemical that reaches that map area they could research the highly reachable areas to find out if adjusting their function would benefit humans, anything from actual medical treatment to causing the highly reachable areas to be what I have read is called better than well

Localization mental illness drugs, keep anti psychotics out of sleepiness areas if they still function

People create ways to keep protein from gunking up tubes, it is possible that unlike proteins, peptides, which can also be drugs, have chemical things which make them much easier to keep ungunkifying, from being able to clean all the peptides off of them off with an enzyme, possibly a deaminase, to drastically fewer gooey nooks at the molecule from having masses and structures up to hundreds of thousands of times less complex, it is possible that peptides screened (or produced) at microfluidics are easy to keep from gunking up areas, surfaces, connectors and geometries that accumulate material, microfluidics technologies at peptides could be clean, fast, durable, and with longer reliability cycles; screening libraries of peptides as longevity drugs with 1/1000th the gunkification at chips could heighten reusability which makes, concentrating, sorting natural peptides better, also absent gunkification the thousands more uses per chip makes it hundreds of times more affordable. From the perspective of making drugs and molecules that benefit people the 2019AD also noting the combinatorial space of peptides is kind of like many factorial and new completely artificial amino acids for peptides have been created, so it is a nonfinite factorial there could be a multihundred times better ungunkiness, tidiness, rinsability at peptide sorting and concentrating chips.

Some peptide libraries that could be mass screened for new longevity drugs: if you divide any protein into pieces about half a hundred amino acids long or eentsier it becomes a peptide so any longevity causing proteins could be made into pieces and screened at yeast in the billions (flow cytometry characterizes 10 billion yeast a second) so if the yeast are engineered to make and accumulate more green fluorescent protein the longer they live, it is possible to find out which yeast have the greatest longevity increase. Longevity proteins could be things like naked mole rat organ homogenate, any electrophoretic band that is at 35 year beavers compared with mice, any electrophoretic band from voluntarily provided, or not-alive person protein differences between humans and other primates, protein bands from termite queens (50- I read, 100 year longevity) not at other termites, the protein band differences between the 17 year part of the cicada's phenotype and its one month long mating form, queen bees and regular bees, the longest lived non s cerevisia yeast and s cerevisia and the briefest yeast, electrophoretic protein bands at the kings holly, 40,000 year lifespan thus far, with the protein not at a few other plants, protein bands at endoliths of numerous species (fungi, algae, cyanobacteria, bacteria) electrophoretic that are not at similar organisms, also noting whale longevity the electrophoretic protein bands at 214 year old whale tissue from non alive whales, or 214 year whale tissue from parturition related tissue in the water, that are absent from other whales; note that there are numerous others and that just these 22 could be macroscopically electrophoresized to get 10-1000 micrograms to dose the yeast with.

Also it would be beneficial when generating the peptides, rather than using enzymes, or even a many enzymes at one flask approach, to divide the proteins into peptides at nonpredictable locations because if you always use an enzyme that divides with lysine then the peptides will all have a distal lysine on them, one possibility is UV radiation, other radiation, or bubbling O3 through the flask

Spectroscopy of isotopically labelled peptides, would a spectroscopy beam at a flow cytometer looking at a yeast be able to see femtograms or picograms of radio labelling from a peptide at the yeast, perhaps, perhaps not, what about a one per 100,000 likeliness of seeing the isotopic labelling and the 20 million yeast per second flow cytometer I read they thought they could make, then that is 200 yeast saying which peptide they have been dosed with per second then that is 630.7 million peptides screened annually, with parallel machines, like 11 of them, that is 69 billion peptides screened for longevity effects. There are 2 1/2 longevity peptides out of that I have heard of, so at an extreme, 1 million published peptides so they might find some from a million isotopically labelled peptides that the first 14 days of spectroscopic flow cytometry of yeast finds, and the flow cytometry of fluorescent proteins notes have highest longevity increase. They could then utilize about 11 96 well plates of c elegans or 6 month fish to quantify the effects on vertebrates of the most longevizing 132 of the peptides.

Use peptide sequencers, and if there are microfluidic peptide sequencers, noting the million channel microfluidic chip i read about, creating combinatorial peptides based on software, like AI, like deep learning AI that predicts effective longevity increase and then makes those longevity peptides which are screenable at yeast.



Precluding protein gunk up at microfluidics, at something like fungal or algal endolith homogenate longevity chemical screening or clam homogenate longevity screening with numerous proteins at the ==‡== microfluidic microelectrophoretic sorter could direct each concentrated, separately tapped band of material at the gel to a microfluidic lane that has an interior polymer (or other material) surface charge that minimizes protein accumulation on the channels sides, at that particular protein's actual characteristics, kind of since you know the charge it is at and responds to you can have it traverse channels where that channel's interior charge is neutral or slightly surface-diffident whichever causes less gunking up

Longevity technology: AEDG (Epithalon) is a peptide published as causing 24% greater longevity at mice, and when administered with thymosin, it makes people 4 times more likely to be alive after 6 years; there are now synthetic amino acids they make, so molecular variants of the A, E, D, G amino acids that are synthetic could be made, assembled into new versions of epithalon and quantified as to longevizing effects; with two new atom swapouts or new atoms per each new synthetic amino acid, that is just 16 binary variants to screen at mice, yeast or human tissue culture, notably this provides a kind of nifty rapid research utilizing human cognition originated drug creation. Noting using a D-amino acid is part of how vasopressin was modified to be orally active a person could enjoy, like, and value making an orally available D amino acid or synthetic amino acid version of Epithalon. Like if I were making a variation, thinking as a human, I would find out if acetylated synthetic amino acid variations on any of the amino acids in AEDG were previously existing, then distal to the part of other things Epithalon they might think Epithalon attaches to or activates things with, I would put the acetylated synthetic amino acid because I think it might cause the acetylated amino acid version of Epithalon to pass the blood brain barrier more effectively, predictably, or pre looking things up online I would find out if achiral (nonchiral) synthetic amino acids have been produced (imaginably a dimer, even like the G at AEDG being doubled, a new achiral glycine)

If this were at a public biotechnology maker space like they have in San Francisco and New York City then it would be nifty to have each of 7 or 14 people each think of their own version of AEDG with the makerspace maker shared enthusiasm and actual craft of making, although possibly just ordering (another pineal gland peptide distally attached to AEDG might have synergistic longevity effects, causing greater longevity increase at mice than the published 24%) things. Also if people at the makerspace knew about AI, or even just the deep learning packages they have, then one of the makerspace people could make a computer program that uses deep learning to create AEDG variation molecules, as well as other longevizing molecules and enjoy doing that, as well as of course wondering how well it would do compared with the human originated versions. If AEDG has a longevity effect on c elegans or more beneficially 96 well plate 6 month fish, then 3 of these plates would give results on the 7-14 people's AEDG versions times 2 or more variants each, utilizing 8 organisms to get a p value, that is 224 wells of fish as well as c elegans to quantify all 14-28 variations.

There is also screening a library to find a more longevizing version of epithalon, from a synthetic amino acid perpective, Modifying the four amino acids to have four atom swapouts per amino acid then that is 2^16 screenable variants, near 65,536 versions; going with the idea they could modify the Epithalon on purpose they could make eight atom swaps or additions each at the two amino acids on the side of epitalon that actually activates things (if there is a receptorside, make the two groups of four atom swaps at the receptorside) then these 65,536 could be screened at yeast as well as human tissue culture to find 99.9th percentile of greater longevity increase from the molecules screened. I do not know if Epithalon makes c elegans as well as daphnia live longer or not, if it does then these could be utilized to compare different AEDG, Epithalon variants.

I perceive Epithalon might be tolerated at a multi-order of magnitude dose range, if it is then that suggests something Epithalon activates gets fully activated, kind of saturated; finding out what that saturated thing is, then making more of the saturated thing, or possibly supplementing, as a longevity drug, the amount of the saturated thing could be a new longevity drug; also i do not know if the saturated thing is a cell type, a receptor at the brain (CNS) and at many places at the body, or a chemical. If it is a cell type or receptor then the genetics of the saturated thing like SNPs, alleles, or copy number variations could be part of the genetics of greater longevity; if it is a cell type or receptor then the epigenetics that effect the amount of DNA transcription that effect how frequently or how many of that receptor gets made could be longevity heightening epigenetics, some epigenetics effects function at multiple generations of humans so it is actually possible taking a tuned HDAC inhibitor could turn making lots more of the saturated thing on, or make lots more of it, and that the heightened longevity effect transfers to your children and grandchildren.

Does making BDNF nerve growth factors or other growth factors at the pineal gland heighten longevity and healthspan.

Other pineal hormones, Epithalon, to my perception was developed from researching pineal peptides, it is possible some of the other pineal peptides are more longevizing but at the time a four amino acid quadropeptide was particularly easy to purify and make, it is possible there are lengthier amino acid sequence pineal peptides that can now simply be ordered to be constructed online and screened at mice to find out if they have greater longevizing effects than Epithalon

Does a 300 year lifespan tortoise have a pineal gland? What about a century bird lifespan bird, could a recently alive, hospice-care like administered multicentury lifespan whale's pineal gland have its peptides sequenced, all of these pineal peptides could be tested at mice to find out if they are heighten longevity above epithalon's published 24%

is it possible to grow stem cells from a multicentury lifespan whale, possibly using traces of GI tract tissue at whale poo as a source, or a plucked hair, and then differentiate the multicentury longevity whale's stem cells with tissue culture to make a source of whale tissue to develop longevity drugs from.

Do 300 year tortoises and 300 year sharks have pineal glands, if they do then their likely varied pineal peptide sequences could be characterized at mice as to longevity increase to find a peptide more longevizing than AEDG, epithalon

Longevity genes and proteins at multicentury whales: at humans, HARs, human accelerated regions are areas of the human genome that have changed and diverged with the most velocity, possibly as compared with other primates, humans living much longer than another primates could be linked to HARs, so at multicentury whales do they have cetacean accelerated regions compared with other whales, if they do then genes causing greater whale longevity could be located among these CARs, humans comparing those CARS, as well as human HARs To each other might find shared homologous genes of longevity (compared to nonhuman primates) with the multi century whale versions of the homologous CAR and HAR genes shared areas being particularly longevizing, the actual gene products, like proteins, of the longevizing versions could be heightened at humans as a longevity technology as well as CAR HAR most longevizing genetics gene therapy, germline gene modification, longevity drugs, it could also be that Whale CARs (comparing nearest genome at other whale species) have a completely non homologous to human genes longevity heightening gene area that could be used to develop new longevity techology as well as longevity genes as well.

CARs and HARs as sources of longevity genes, could be sources of longevity genes that benefit humans even when they arise at other species, imaginably noting epithalon's 24% longevity heightening effect, and that i think brain development areas are among the human accelerated regions, HARS, it is possible if you make pineal effecting HARs genes part of the mouse genome the mice might live longer

Complemtary

Thinkable: noting three of them as a proof is it possible, along with longevity with healthspan and wellness and being simultaneously benevolent and beneficial and effective at raising children, that there is anything at humans where a larger amoumt of it is always beneficial? This could actually have a mathematical interpretation where some axioms about iniverse math and topology create different and beneficial math limned options;

Getting around all the math of how varieties of sharing can occur at different math systems, dividing odd numbered things on a curved surface or a planar surface causing equal, unequal, matrix equal but anisotropic kinds of partioning, as well as the mathematics of nonpartitionable systems, also interconnected or stand alone systems where all agents always have perfect information arising from the math definitions of the system they are at, consider a person with 360 spherical vision living comfortably in a pleasant easily climbable bowl, who has used science to verify that like 20th century humans communicating the, to my perspective, MWI modified idea, that there is no thing space expands into; the person with the 360° spherical vision actually, at that math space, sees it all, and has the mathematical topological space of having perfect information; the "outer surface" of the bowl is absent detectability with physics, and to have the math function with everything that has ever been measured supports there being no exterior of the bowl (gideon's trumpet, nonfinite length, enumerable surface area possibly is reminiscent of a bowl with a math space where there is a region of presence and non presence at a thing); finding new math spaces that optimize human well being is beneficial, because they make worlds where polythings align with goodness

With math as a beautiful prompt, are there things that if there were heigtened amounts would always be beneficial; well i think it is possible to create a math space where kind of like a topology of all beings having perfect information (360° spherical vision unisided dish), any recombination always produces a more beneficial change in form than omitting any kind of recombination or sorting, while the previous to change universe, at that mathematical topology, is to any sentience fully optimal; any change could heighten an aleph number that a mathematician describes the universe' topology with, one possibility is

So thinking about where i have presence of being, is there a heightened amount is always beneficial

If i can just make atoms, is that a more is better thing, anyone can use them, and noting that the universe is good it just causes a greater amount of universe, using math the new atoms could have equational creatability at various equational areas of the universe, sort of like the way mathematicians are able to definitionally limn an area where "you can add 2 to anything and it will retain its odd or evenness" there is a math system like a topology where it is possible to put more atoms, at a kind of

This is different than the kind of langiage effect "can i find an exception" this is making new math environments where if you say torus then another person saying, thete are two or more circumferences is the way it goes( there is an exception though, if you put a gravitational singularity at the middle of a torus then that middle circumference ballons towards it and at 1 dimensional numbers a balooning occassion causes the middle circumferance to be identical to the outer circumference;

A computer scientist might say, oh, what you mean is algorithms with proofs that limn their behavior, and, among other mathematical forms, i think you would like thinking of mathematically limned "proofed" algorithms that, noting the universe is good, make the universe gooder, so you are utilizing a moment to think of thimgs where at any mathematical or definitional space a greater amount of any anything is gooder;

Whether it has an aleph number or not heighten the universe' aleph number

Just make more atoms, is there a way to figure out if just makimg bosons or just makimg fermions is gooder, have you asked aroumd about a greater quantity of spontaneously resolving quantum objects or also a greater quantity of quantum objects that are unresolvable

Is there a gooder quantum object like a linear photon that gradually builds its brightness so that any organism that liked it could experience it, or if they felt sleepy could just shut their eyelids and go to sleep, , or a true analog thing that is. Planck unit nondependent

If you have a network of n objects such that when superimposed the additive wave is always positive and high thing to the³ compared with thing² and the top half of ac is at an etalon and the container size and when I say container it could just be a parabola of frequencies with the upper part of the parabola a wave frequency larger than the span of the universe and the optics such that only optimal images would retroreflect

A parabolic mirror at a math space where everything was reflected twice at a frequency doubling crystal such that if it were reflected once it would traverse to outside the network and diffuse to the same frequency as the nearest most light emitting object, then shape the topological space so that always happened, all the images viewed would be right side up and the right handedness would be at each image the reflections would be a clearer image each time because they are vector graphics, a casimir flashlight could provide the light that gets

Casimir flashlight automatically motionizes vector graphics

Casimir flashlight attached to an oscillator always gives the oscillator a nudge with some surplus and the surplus makes the parabola higher and raises the curve at the base or at a math space does the up widening traverse thing as it is topologically gyreless, the parabola lifts at the same velocity the math space expands so the proportionate location does not change while the vector graphics reflect twice all the time with the raising and expansion causing a larger number of vector graphic reflections of higher resolution while (at a size changing topological universe)

This could also be accomplished with a gradient refractive image lens at a plasmid shaped topological universe, any occurrence at the sides of the lens would be at total refractive index, and the network nodes at the interior of the tir would experience connectivity without reaching the perimeter; an optical computer in the middle of a curved optic with 3d hat wave, egg cup, such that the tir caused retro reflection that was also a vector graphic; so what the network experiences is heightening vector image quality as the parabola lifts and heightens while the nonperceptible tir at the sides of the plasmid expanded, the duration of the reflected vector graphics is such that any node of the network was equidistant from a light patterns gradual and normal pace versions, the cofocalization of the sides of the parabola would cause the connectivity of the network as well as the nodes to accumulate presence of effect in phase with the network nodes that were around, with math I think you can have a simultaneous group of equations that does that, casimir flashlights work in water and the light would accumulate in place if there were no nodes, noting million year endoliths the nodes would usually be there and they could process the vector graphics from the sides of the parabola or each other the casimir flashlight could actually be a coating,



Thinking about math, although i perceive observing a new gooder thing and heightening the quantity is a gooderness maker

Is it possible; what is a way to observe a new gooder thing?

Well, you could find people that observed new gooder things, then with technology simulate them nonsentiently then actuate a trillion of the nonsentient simulations, then broadcast the new gooder things they observe at a math space of

Simultaneous linear equations of the amount of streamlining, also words that happen at my mind that are 40% or less of what i actually thought, although these 40% words may have a participateable theme of their own, intimations of pattern meaning from form being different

A drug that decreases streamlining some number of units, while being actually recognized as heightening absence of streamlining whete teduced externsl patjwayization is like 900 units of less automatic so another person bringing up an opportunity for a voluntary action would have 90 0units of greater possible voluntaryness this simultaneous equation space where automated respone forms habe less prompting while a new beneficial thing is questing agter psrticipation contributes to a mathematics supporting the possibility of voluntary action (like math supports physics) notably there could be many such drugs which happen to cause gooderness at most of the cumulative occurences, also there are some drugs that cause, at me, a high, while also decreasing streamlining and possibly 40% thoughts, a drug that might do that is phenibut, a nonintoxicatimg drug that removes the abridgement of actual feeling and thouhht of intent 900 units while a different benevolent thing is alignig 900 units of voluntariness as utilization capacity with the change like a beneficial thing, they favor

Genes of openness to experoence; genes of the words in your head being some percentage of what you mean, like actually 100% people, 90% people, 40% people being genomically compared and then their genes used to predict the statements about"words in my head are(amount)% that I mean", that a sample of people with genomes at a database make; when the computer model of the genes that cause some version of this is 95% predictive then the genes of 100% of the words in your head actually mean are promoted, perhaps even made available as a sample with gene therapy or drugs, then to the amount they can, people can purposefully opt for, along with their previously actually knowing what they mean, what they thought, and perhaps for some what they intend, the actual words in their head, like casual narrative thinking, such that then articulate that

It is my perception that the debrisifying or content mutation or content simplification that occurs when the words in my head are at a 40% thought that the actual capacity or ability to be simultaneously cohered at a voluntary action opportunity, so it is more actually voluntary like numbers derived from simultaneous linear equations solved amounts of the possibility an act is voluntary gets greater the more the words in your head are 100% what you mean;

Somebody reads an advertisement for something that is actually beneficial, if the words in their head are 100% of what they mean, and they actually get the beneficial advertised object, then their capacity, like their available amperes of voluntary, is more voluntary than a words at my head communicate only 20% of what I mean person, so this is genetics and drugs that heighten the capacity of humans to be less encumbering to others sense of wondering if they are being fair, if a 100% person volunteers it may actually be more ethical quantified like amperes (compared with ethical the dictionary definition)

genes of

It is possible a nuclear chain reaction, noting it always decoheres more things than it resolves, continuously might be a simple sytem that is unresolvable, a person could only ever say they saw part of it; also some of them are autocatalytic; what is the eentsiest always quantum unresolvable system that humans also like, white light, being physicsy, is definitionally omitting of sufficient observation to be described as its components, although there could be a math thing, or even a physics thing like we emitted some white light so we know how many watts occured,

Stomach lining 72 hour rrgrow


Treon Verdery

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Klotho is a kidney gene that makes mice live longer and be twice as effective at finding things when genetically engineered to make a lot of it , it is possible that organisms that utilize their kidneys and brains a bunch simultaneously could have more effective versions of klotho, dolphins, whales and sea otters are organisms that may have this feature from gulping ocean water and processing it, beavers might gulp a lot of freshwater and as they build things may benefit from higher cognitive ability, klotho could, possibly or possibly not, contribute to beavers living 35 times longer than mice, squirrels which have orders of magnitude greater ability at remembering and finding objects, to my perception, compared with mice I previously mentioned as Klotho gene versions, with possible optimizability, as well as slight variations on the gene protein product as a protein drug, a longevity drug as well as a variation as a protein drug nootropic

Longevity technology

Klotho external protein is mentioned as receptor less but concentrating at a word like sialogangliosides, that suggests sialogangliosides could be tested as longevity drugs and nootropics

Supercentensrians were tested and found to have about 1.5 times greater likeliness of having the most favorable variant of the klotho gene




Treon Verdery

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Treon Verdery

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With higher IQ linked to greater longevity they could do brain scans of super centenarians and find out if their actual brain mass at each millimeter of brain structure varies from others, quantifying positron emission tomography amounts of radio labelled chemicals the supercentenarian variation at what amount of actual neurotransmitter chemical is where as well as neurotransmitter receptor density is describable, and could be associated with longevity, and longevity chemicals active at mouse brains, complementary to while being different than nootropics could be screened and produced; non cognitive areas of the brain could also be heightening longevity (Epithalon, a pineal peptide, is published as causing 24% greater longevity) new longevity drugs developed to address brain areas that might be outside cognition are also beneficial



Screening stimulant molecules to find out if there are longevity heightening molecular variations:



On finding 99.9 th percentily of longevity heightening stimulants at 96 well plate fish, screen the 99.9th percentile stimulants on mice, quantify absence of emotive or also social conflict, optimally less than the amount of median unmedicated mammal emotive and social conflict, also beneficial is quantifying mouse actions, communicating, feeling happy, play, and willingness to travel at open spaces, another the benefit of a longevity producing stimulant could be quantified conviviality, also mating frequency,



Stimulants at less than a behavior changing dose could also cause greater longevity particularly screened as a library at yeast as well as c elegans;



At perceptible stimulants a voluntary human experimental participant verifies the longevizing stimulants as pleasant and fun then marmosets at age batches verify longevity effect while having fun



It is beneficial to

Screen longevity stimulants as beneficial or neutral to the fetus, or are also absent passing the placenta



Rapamycin is published as causing 60% greater longevity as well as higher cognitive performance at 126 ppm at mice, I took some at kind of near 24-63 ppm and noticed it made me kind of excited so I did not go to sleep, so I started taking it, when possible, before or near noon, it is possible that screening a library of rapamycin molecule variations to find nootropic or also beneficial prosocial stimulant versions could be even more longevizing than rapamycin (60% rapamycin with near 20 from IQ is 80 pct longevity heightening) stimulant ability of rapamycin molecule variants could be screened at c elegans to see how fast they move around before quantifying the effects on longevity, social behavior and cognition at mice;

attaching a nootropic or stimulant moiety to rapamycin could put the 21% greater longevity from higher IQ with the 60% longevity heightening from rapamycin to make a drug with 80 pct greater longevity effects that could be quantified at age batched marmosets



Epinepherine I heard, is aversive, it might be linked to heightened cognitive function as I perceive I read some humans get hyperfocused, more simultaneously aware, and mentally and physically faster possibly at a voluntary epinepherine producing activity, screening a library of numerous epinepherine variants could find a fun, harmless, pleasant, peaceful, multiarea beneficial longevizing version



Organoids as tissue culture forms that could be utilized toscreen chemical libraries, positron emission tomography on tray stacks, 3-7 mm per well, 49 rows columns and stacks per positron emission tomography scan occurrence scans 117,000ish verified as being absent isness organoids



Is a way to verify absence of presence of being, like at 3mm neuronal brain-like nuggets like organoids to position the thing being qualified as absent presence of being as a quantum system observer, then if the system is observationally resolved there is possibility of presence of being, if the thing is absent quantum resolution then it is possible the object being qualified as to absence of presence of being like an organoid is either not being attentive, like a human that is so romance focused they fantasize about their girlfriend while looking at the quantum apparatus and do not notice what is going on, or rather than nonattentive it is possibile the organoid is actually absent presence of being, while quantum resolution as compared with a 3mm nugget of a different tissue would make it so they built different organoids that were absent quantum resolution capability



organoids with reduced likelihood of having presence might be built with a matrice of neurotransmitters, neuron types, or morphologies omitted; screening things at organoids matricized to have serotonin with acetylcholine but not dopamine at one group of organoids, while a different group of organoids has a different neurotransmitter matrice, causes nonbeing form when screening a million organoids at chemicals like beneficial longevity drugs;



Longevity technology as well as new longevity drugs



Nootropics

Scientific American might say button pressing reaction interval is more predictive than iq, or possibly fully elucidates iq causing greater longevity, that makes it so that stimulants as well as nootropics could have a longevizing effect; screening molecule variants of stimulants on yeast, c elegans, drosophila and mice to find stimulants that cause greater longevity is beneficial



At mice, screening stimulants to find those that are prosocial, peaceful, creative, productive, as well as fun is beneficial, human study participants on one occuremce doses could also be quantified as to various positive measures of beneficialness



Notably, different stimulants function different ways. Noting the button press predictor of longevity it is possible that stimulants that effect the peripheral nervous system, even those that do not traverse the blood brain barrier could be longevity drugs, CNS stimulants could also be quantified as to longevizing effects as could stimulants that effect both, also I perceive there are other nervous systems like somatic sensory nerves that could have stimulants focused on them and quantified as to longevity effects



Perhaps localization (published and new cyte and tissue localization technologies, as well as cytomembrane transport moieties) of stimulants also contributes to longevity effects, particularly longevity heightening effect nerves, possibly the vagus, the pancreatic nerves as well as spinal cord; localization could reach these while omitting effects on the heart like rapid pulse or blood pressure effects, decreasing any deleterious effects



Stimulants could function based on different parts of neurons and it could be possible to find neuron areas that create a modality of stimulant type, a stimulant theme to make new screenable libraries with that heighten longevity; dendrite active stimulants. Synapse active stimulants, myelin enhancement that is stimulating or also makes button pressing reaction interval, as well as organism wide neural reaction interval more rapid are all screen able as different possible longevity drugs;

Screening stimulant molecule varieties that are lipiphilic, hydrophilic, neutralphilic, as well as different sizes of AMU molecules are possible screenable libraries to find longevizing effects; also the 99.9th percentile of longevizing activity at c elegans or 96 well plate vertebrate fish stimulants could then be screened on mice, possibly sequentially every 72 hours to find beneficial benevolence, empathy, kindness, peacefulness, cognition, fun heightening molecules, then the quantified effects at mice could produce beneficial longevity heightening stimulants that voluntary human experiment participants could quantify with things like reactions to software and fMRI similarity to humans at the 99.9th percentile of beneficial mind, feeling, behavior and cognitive capability;

Noting the higher velocity reaction intervals link to longevity it is possible nonchemical nerve velocity heightening technologies could be longevity technologies; microvoltage or micro current stimulation, EEG like non CNS electricity directing and causing EEGish frequencies like those recorded at 14 year olds could cause non CNS nerves to make the kinds of reoccurring waveforms at the same locations as particularly youthful, or actually (14 year old) youthful people, entire organism EEG like stimulation is described at the online site with my material on it, possibilities are ankle as well as wrist wearable through-body stimulation as well as possible electrochemically active .1 to 1 mm CPU size networked electrical waveform network making decal technology



Making screenable library variants to find longevity heightening stimulants based on these chemicals:

Modafinil

ADD drugs,

Phenylethylamines, pikhal shulgin

MDMA, prosocial. fun

Stimulant molecular variants of rapamycin

CART peptides

Ephedra

Epinephrine molecular variants that are pleasant

nonhabituating +Caines, procaine has about one study quantifying wellness and longevity benefits, screen 1000 nonhabituating -Caine's at outdoor mouse dorm mice



Screening a million stimulant molecule variations on yeast could find longevity drugs even though yeast do not have nervous systems, among the possibilities are multifunction molecules with another longevity mechanism HAEE is an 10HDA(longevity) like molecule that causes anxiolytic and nerve healing effects, it is possible there is a stimulant molecular variation of HAEE, possibly even a decanoic acid ester, with simultaneous 10HDA longevity effects, HAEE anxiolytic and nerve healing effects and stimulant based longevity heightening effects; similarly 10HDA is an HDAC inhibitor, it is possible some variety of an HDAC inhibitor or other epigenetic modifying molecule that causes genetic availability of spontaneous stimulation, heightens stimulant ability, or is also a molecularly active stimulant as an actual chemical



Longevity technology as well as new longevity drugs



Nootropics

Scientific American might say button pressing reaction interval is more predictive than iq, or possibly fully elucidates iq causing greater longevity, that makes it so that stimulants as well as nootropics could have a longevizing effect; screening molecule variants of stimulants on yeast, c elegans, drosophila and mice to find stimulants that cause greater longevity is beneficial



At mice, screening stimulants to find those that are prosocial, peaceful, creative, productive, as well as fun is beneficial, human study participants on one occuremce doses could also be quantified as to various positive measures of beneficialness



Notably, different stimulants function different ways. Noting the button press predictor of longevity it is possible that stimulants that effect the peripheral nervous system, even those that do not traverse the blood brain barrier could be longevity drugs, CNS stimulants could also be quantified as to longevizing effects as could stimulants that effect both, also I perceive there are other nervous systems like somatic sensory nerves that could have stimulants focused on them and quantified as to longevity effects



Perhaps localization (published and new cyte and tissue localization technologies, as well as cytomembrane transport moieties) of stimulants also contributes to longevity effects, particularly longevity heightening effect nerves, possibly the vagus, the pancreatic nerves as well as spinal cord; localization could reach these while omitting effects on the heart like rapid pulse or blood pressure effects, decreasing any deleterious effects



Stimulants could function based on different parts of neurons and it could be possible to find neuron areas that create a modality of stimulant type, a stimulant theme to make new screenable libraries with that heighten longevity; dendrite active stimulants. Synapse active stimulants, myelin enhancement that is stimulating or also makes button pressing reaction interval, as well as organism wide neural reaction interval more rapid are all screen able as different possible longevity drugs;

Screening stimulant molecule varieties that are lipiphilic, hydrophilic, neutralphilic, as well as different sizes of AMU molecules are possible screenable libraries to find longevizing effects; also the 99.9th percentile of longevizing activity at c elegans or 96 well plate vertebrate fish stimulants could then be screened on mice, possibly sequentially every 72 hours to find beneficial benevolence, empathy, kindness, peacefulness, cognition, fun heightening molecules, then the quantified effects at mice could produce beneficial longevity heightening stimulants that voluntary human experiment participants could quantify with things like reactions to software and fMRI similarity to humans at the 99.9th percentile of beneficial mind, feeling, behavior and cognitive capability;

Noting the higher velocity reaction intervals link to longevity it is possible nonchemical nerve velocity heightening technologies could be longevity technologies; microvoltage or micro current stimulation, EEG like non CNS electricity directing and causing EEGish frequencies like those recorded at 14 year olds could cause non CNS nerves to make the kinds of reoccurring waveforms at the same locations as particularly youthful, or actually (14 year old) youthful people, entire organism EEG like stimulation is described at the online site with my material on it, possibilities are ankle as well as wrist wearable through-body stimulation as well as possible electrochemically active .1 to 1 mm CPU size networked electrical waveform network making decal technology



Making screenable library variants to find longevity heightening stimulants based on these chemicals:

Modafinil

ADD drugs,

Phenylethylamines, pikhal shulgin

MDMA, prosocial. fun

Stimulant molecular variants of rapamycin

CART peptides

Ephedra

Epinephrine molecular variants that are pleasant

nonhabituating +Caines, procaine has about one study quantifying wellness and longevity benefits, screen 1000 nonhabituating -Caine's at outdoor mouse dorm mice



Screening a million stimulant molecule variations on yeast could find longevity drugs even though yeast do not have nervous systems, among the possibilities are multifunction molecules with another longevity mechanism HAEE is an 10HDA(longevity) like molecule that causes anxiolytic and nerve healing effects, it is possible there is a stimulant molecular variation of HAEE, possibly even a decanoic acid ester, with simultaneous 10HDA longevity effects, HAEE anxiolytic and nerve healing effects and stimulant based longevity heightening effects; similarly 10HDA is an HDAC inhibitor, it is possible some variety of an HDAC inhibitor or other epigenetic modifying molecule that causes genetic availability of spontaneous stimulation, heightens stimulant ability, or is also a molecularly active stimulant as an actual chemical



It is published that longevity goes up 21% at the upper 32% (including higher amounts) of IQ, also longevity and being alive things that could likely be brain processed, like if a romantic partner is alive and the amount of friends as well as the social experience of a person effect longevity; drugs and genetics that enhance brain function are longevity technologies and drugs;



New longevity drugs based on nootropics (smart drugs), Screen a million or more nootropic molecule variants at yeast, c elegans; they may have longevity molecule actions that complement their cognitive effects, then screen the 99.9th percentile of longevizing nootropic sourced molecules on mice to find those that simultaneously heighten longevity, subjective well being as well as cognitive ability to make beneficial new human drugs



Phosphatidyl serine and variations as nootropics as well as quantifiable longevity drug; other phosphatidyl amino acids and different molecule alkane lengths could be screened as a library



Screen a library of a million nootropic molecule variants and find the molecules that simultaneously heighten longevity, subjective well being like happiness, and cognitive function, then test the 99.99 percentile of them on mice, make them human drugs



All the greater than 100 neurotransmitter genes and all the brain morphology genes could be characterized as to their effect on longevity, heightening subjective well being and kindness as well as heightening cognitive ability; the simultaneously multibenefical effect chemicals, like proteins, the genes make are library screened as protein drugs, those at the 99th or 99.9th percentile of multi beneficial effect are then beneficial human drugs;



it is also possible that among the greater than 100 neurotransmitters there are three where receptor activation is of greatest benefit as well as three where receptor activity decrease causes benefit like greater longevity, happiness as well as cognitive ability, drugs that effect these nifty and antinifty neurotransmitter types are then longevity, subjective well being, as well as cognitive ability increasing drugs, and genetics of the nifty neurotransmitters are genetic enhancement opportunities at humans as well as are the antinifty neurotransmitters activity being genetically reduced, which then heightens longevity or also happiness or also cognitive ability at humans



Bile acids cause yeast to have doubled longevity and increase the maximum age attained; screening a library of a million molecular variants on lithocholic acid at yeast is a way to find new longevity drugs; Finding the 99.9 the percentile of molecules that heighten longevity at yeast then screening them at fish as well as mice, then making human drugs creates new longevity drugs; among numerous molecules variants screenable, along with combinatorial approaches, topology theme variations (undulating 3dness at molecules, near planar molecules, rapidly or gradually repeatedly changing shape (like chair boat at benzene), possibly there are variations at hydrophobicity that happen to also be orders of magnitude differenct from each other at lipophilicity, dimerization at different sides of the molecule, protein versions, that to my perception can actually imitate few AMU drugs, making the distal thing that looks like a butyl to be propyl, heptyl, 6,7,8,9,10 C long (notably longevity molecule 10HDA(10H2DA) could be the distal thing), making the cyclohexane like parts of the molecule unsaturated (C=C areas, like partial benzenes) as well as making them cycloheptyl (7 atom circle) vesions at some of them, removing or replacing the methyl branch with an ethyl, propyl or other lipophilic alkane, changing the =O at the ester to a sulfur, as well as replacing the carboxyl with an amide (less hydrophilic), screen able variants like fluorine or other halogen where the hydrogen's are (fluorine might be notably hydrophobic from the teflonization I perceive possible, also some fluorinated drugs have 1000 times greater activity per mg, enhancing pills/dosing as well as heightening affordability possibly 1000 times, also ethynylization, noting lithocholic acid is cholesterolish and that ethynylizing cholesterolish sex hormones like progesterone makes them hundreds of times more active and active at a few hundred picograms per human dose; noting that sex hormones are cholesterolish, lithocholic acid variations with similarity to the published longevity drug, nonfeminizing 17 alpha estradiol which causes 11% greater longevity could be among the screenable variants



Complementing screening a library of lithocholic acid variants, making radiolabelled versions and at the 14 most longevizing variants out of a million find out which cytoareas, receptors, as well as genetics the 99.999th percentile of heightening longevity variants effect



three or fouteen genes producing differing bile acid molecules linkable to human longevity could be longevity genes

Nootropic stimulants

Longevity technology



At longevity chemical and drug screening is also possible to utilize informed, voluntary, capable, humans, that is persons, that is members of groups of people, that is homo sapiens as voluntarily participating paid participants: utilizing psychological testing it could also be possible to heighten the happiness of the volunteers; it is possible to utilize only 70th percentile or greater of persons at their society's quantified feeling and thinking that they have options, surpluses, and other opportunities:

A person making the reusable psychometric product, likely software, finds people above the 70th percentile of fiscal resources, as well as above the 70the percentile of preferred amount of fiscal flows at that society, and quantifies them psychometrically, then at a different group, the group of people that would like to participate, at that group of people they also find the people at the median quantified feeling or above, of that of the 70th percentile group as to their feeling fiscally of perceiving they are median or above at big 5 psychology test measured mental wellness, they are also screened on if they are at or above the society's median at both intelligence and educational hours experienced; these people perceive they are doing well, do what they offer, can comprehend the purpose, documentation risks and benefits of being a human experimental subject; heightening the happiness of the participants is also possible, a 16 to 40 item optional psychology test is available, the quantifications from they test have an r value of predicting the effect of experiment participation among people replying at various ways on the psychological test, then they get to participate based on their percentile of how much happier they are projected to be, that is, their quantifiable subjective well being, from participating as a human test subject at that actual experiment;



I favor financial compensation to humans that voluntarily participate at scientific studies including medical studies.



Actinomycetes also is modified to be a nutrient source, as well as possibly a habitat for the fungi and bacteria that globally produce longevity heightening drugs that are effective on humans as well as numerous other organisms, that way from the fungi and bacteria living at the actinomycetes that lives at the earthen particles(dirt) the earthen particles continually, at minute amounts, like the amounts reaching leaves of plants at 2019 AD earth, cause the surfaces of plants to have longevity chemical and drug molecule producing bacteria and fungi on them, possibly as entire fungi and bacteria as well as spores, noting the bacteria and fungi are harmless to the plant unless kept wet with unmoving water for 72 hours, noting that rain is moving water, rather than the unmoving water that accumulates bacterial and fungal chemicals various lengths of rain omit causing the fungi and bacteria to digest the plant; the fungi and bacteria grow, activate, and make longevity drugs and chemicals when at unmoving water 72 hours;



Rapamycin at 126 ppm that causes 60% greater longevity causes more mouse head lifts as well as better cognitive performance; possibly, it could be that head lifts are moments of noticing, or a pleasant mood causing the action; as a human taking about 40-60 mg of rapamycin every 24 hours I noticed near sleep hour rapamycin causing being too slightly enthused and alert to sleep, while socializing while it was light I perceived I responded to the other person more promptly, was absent any new or novel emotional valence, and I experienced a moment when I felt I would have liked it if the person I was socializing with said funner newer things more rapidly, but that was like a few seconds; I experienced the rapamycin as being emotionally non modifying, less stimulating than caffeine, as well as harmless; it brings up a technology of screening longevity drug molecular variants on their projected and actual effects on beneficial voluntary being, form and activity at human mind and behavior;

Screening a library of longevity drugs as to behavior effects, presence of being, as well as being physiological wellness heightening or wellness neutral at 72 hour dosing technology: Screen a library of perhaps 100,000 rapamycin or other longevity drug variants at say 33 mice, with sequential screening of the longevity chemicals to characterize the mice' behavioral, social, fMRI, Positron emission tomography effects; one different chemical per each 72 hours, with the mice on video and their tissue scanned a couple times, is near 300 chemicals quantified per mouse, at a 2019 AD unmedicated mouse; so that is 100k longevity chemicals sequentially quantified with 40ish mice,

To find pleasant or also neutral behavior and mind effect longevity chemical molecules the mice could be screened for a few hours of being medicated at group size: one mouse, before screening any particular chemical molecule on the 8 mice per molecule that generates a p<.05 p value, screening the longevity chemicals at mice to verify beneficial positive or neutral effects on mind and behavior prior to characterizing and screening them at marmosets makes it so the majority of mice and the primates have beneficial, positive, or neutral mind effects and behavior effects; noting these longevity drugs are technology to benefit humans, that is persons, that is members of groups of people that is homo sapiens quantifying and screening to find beneficial positive effects has actual value;



Noting the longevity chemicals are longevity beneficial, 33 marmosets could screen 700k molecules or higher amounts sequentially while providing primate data that could be more predictive of human prosocial, pleasantness of experience, as well as a variety of beneficial things like actualized behavior as well as mind and feeling and sustained presence of benevolence, kindness, empathy behaviors and ways of mind as well as fMRI of mind (like find 99th percentile benevolent empathic kind humans, do fMRI, then at the marmosets note which longevity drug molecules align the fMRI of the marmosets with those of 99th percentile benevolent, empathic, kind humans; also at a different quantification find the longevity drugs that cause, at the marmosets, actual behavior or also fMRI well above the human actual behavior or also fMRI at greater than the previous unmedicated median voluntary amount of: sharing, mating success, above median social fluency, initiating what to a human would be friendly contact, big 5 psychology test openness to experience, conscientiousness, mental wellness, as well as fMRI of creativity



Making longevity drugs globally available, the 24 most frequently occurring kinds of bacteria as well as fungi that live on wet decaying leaves, also wet decaying grass are engineerable with CRISPR/cas9 gene drive to make longevity drugs, the most frequently occurring soil bacteria, among them actinomycetes, are also gene drive modifiable to make longevity drugs, making dirt water longevizing, fungally produced rapamycin, Epithalon AEDG peptide, mTOR decreasers, senolytic fisetin, e coli producible antibodies cause insulin like growth factor 1 reduction, decreased response at the ILGF-1 cytosurface receptor from antibodies on it, are all producible at organisms and gene drive at organisms that cause wet plant material to decay; the person just puts some water on plant parts, puts it in a bag and when the sweetness peptides the genetic engineering produces make it taste delicious, perhaps with an enzyme that neutralizes other flavors, the longevity drugs are orally utilizable





Also mTOR decreasing active proteins

mTOR receptor response reducing active noncyto nonmacrophage glomming of entire cyte receptor glomming antibodies



Screening longevity chemicals and drugs sequentially, when the 100-700k chemicals are sequentially screened with the mice or also marmosets at a weather ameliorated (canopied) outdoor mouse dorm as well as outdoor marmoset dorm then the mammals can move and socialize heightening social data value



Lithochilic acid causes yeast to live twice as long and heightens maximum lifespan, screening a million molecular variations on lithocholic acid could create new longevity drugs; at humans, humans that have greater amounts of lithocholic acid have higher cancer risk, I read mice tend to get cancer more than humans so when screening the 99.99th percentile of the most longevizing molecular variants of lithochilic acid it is possible a mammal lthat has less cancer risk than mice, as well as age batched marmosets could be a better model for quantifying longevity increase; an online item says lithocholic acid is toxic but not at humans when medically adminsteref, it is also possible that at humans the coadministration of cancer risk reducing longevity chemical like metformin or rapamycin could cause the actual cancer risk of rabbits as well as mice as well as marmosets to be less that that of completely unmedicated rabbits, mice, or marmosets; cholesterol risks could be reduced to less than those of unmedicated mammals with the high density lipoprotein cholesterol reducing drug simvastatin which is published as having a longevity benefit, although possibly from heightening healthspan



Alibaba lithocholic acid dose; at humans it is published that 30mg/24 hours of ursolithocholic acid causes 16.86 micromolar concentration ( up from .05), and bile acid amount went up to 17.21 (all the bile acids) suggesting that 50 micromolar concentration like the yeast that live twice as long is a 89 mg/24 hours; although it might not be a plausible dose: the paper says 30 mg of ursolithocholic acid causes only .22 micromolar concentration of lithocholic acid at humans; perhaps the 17.21 millimolar concentration of all kinds of bile acids, 6 of which make yeast live longer, compensates for that; another paper says ursodeoxycholic acid (a hydrophilic bile acid at ( 30mg/kg each 24 hours) makes previously unwell humans 2.1times more likely to omit being alive than unmedicated humans, note though that that is at 30 mg/kg/24 hours so is 70 times higher than the 30 mg makes 17 micromolar concentration at humans so it is possible that a 1/70th dose has only a 1.03 (compared with one) likeliness of causing not being alive;



it is possible that doing correlative studies of human longevity from the varying amounts of bile acid that occur at the different phenotypes at the human population could find genes that make concentrations of bile acid molecules different than others, if there are versioms of three or fourteen different kinds of genes that effect bile acid actual different molecule amount then those three or fourteen genetic variations, their SNPs, alleles, and copy numbers could correlate with different human longevity, also they could look at the genetics and measured amounts of bile acids at super centenarians to find out if there is a more longevizing genetics of bile acids or amount of bile acids





I do not know if there is a yeast compensation number when calculating a human dose from a yeast dose like the order of magnitudish less drug compensation number at mice



Write about lithochilic acid online like longevity.org















Treon Verdery

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Oct 2, 2022, 8:03:50 AMOct 2
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Rapamycin is a nootropic that can heighten laboratory mammal cognition and can keep me awake, brain positron emission tomography could find out which neuron types, and at what brain location these effects occur from, other nootropics that activate these areas could be screened for longevizing effects, also if rapamycin is concentrating at some regions of the brain, or some neurotransmitter types and not others then a molecular variant of rapamycin that goes to all brain areas and all neurotransmitters could be found



Longevity gene, neuron types where one neuron uses a particular neurotransmitter or just all the cytes at the body could use epigenetic modification drugs to make fewer mTOR receptors and possibly increase the amount of AMPK receptors at neurons, causing the CNS to be more youthful possibly causing longer duration of youthful mind, and youthful cognition, and youthful emotion, cardiovascular system cytes could also be beneficial, causing greater longevization, youthspan of the heart and vasculature, this could be a one dose drug that prevents cardiovascular disease



Create epigenetics that reduce activity of mTOR receptor producing genes, and possibly heighten production of AMPK from AMPK genes, possibly through screening a million or to find an even more optimal epigenetic modifier, tens of millions of molecular variants of epigenetic modifying molecules to one that is most specific to downregulating mTOR, and up regulating AMPK, this could have multi generation longevizing effects, germline or also oocytes and spem modification to have the epigenetics of longevity is beneficial



Modifying the genetics of cytes used at nuclear transfer to oocytes to have mTOR epigenetics that cause greater longevity

Liposomal ultra micro reactors

Aluminum or other atom hydrophilic or electrically charged head, or at another solvent like NH3 or DMSO, Or silicone oil, charged silicone distal electronegativity oil, make liposomes or things like silicosomes with reactants at the liposome or silicosomes cores, sequential liposome or silicosome dipping might even combine reactants that are non able to occupy the same flask, then a detonation, warmth occurrence, photochemistry, like lasers event, or radiation occurs and the comparatively few atoms interior to the liposome react to form a product at the core of the liposome, liposomes are kind of see through and liposomes can be sorted with a spectrophotometer at a thing like a flow cytometer, or possibly liposomes or silicosomes could have surface charge that varies with core contents possibly from making the silicosome with 1/100th highly C=C saturated with NH on it or zubbles (TM a kind of plaything) like chalcones, which are so responsive that without disintegrating they change color based on surface tension) then based on what is in the liposome the surface chemistry differs facilitating sorting, a trillion liposomes with 1000 reaction production product molecules each makes about 2^14 atoms or a billionth of a mole, at a 100 amu product that is 1 microgram of product, flow cytometry to centrifuge



Silicosomes can be made with solventophilic heads and multimers with electronegative (like nitrogen, phosphorus, metals, Se ) atom heads, it is possible to omit carbon funkiness (what happens after an actual lipid liposome is warmed or disintegrated with light or radiation), the non interactivity of the reactant molecules with the Si silicosomal interior perimeter makes things cleaner



The reacted silicosomes do a laser spectroscopy and flow cytometry like traversal of a tube and are sorted into products, so a reaction that yielded 100 products then produces 100 different separated products, possibly a million at a microfluidic device (a million channel microfluidic chip I read about), and if these are products like longevity chemicals they can then move to an organism area to find out if they are longevizing, so 1 million varieties of halogenated rapamycin can be silicosomal reaction produced, spectroscopy sorted, then applied to yeast or human tissue culture microfluidically, a trillion silicosomes could be 1 microgram of product, at a 300 mm wafer with 10-100 billion wells per chip, 1 million variants of longevity molecules tested per notable source, things like endolith chemicals autovariationized is 10k sou



Longevity technology

Yeast have longevity chemical responses, find another organisms, possibly even another yeast, that responds like yeast to a library of 10k different chemicals but has a 4 hour lifespan, also verify that when tested at the 4 hour lifespan 40-100 different longevizing remain longevizing, then use that organism to screen new longevity chemicals in just 4800 minutes to 240 minutes each, so at the reacted sorted liposomes microfluidically connected to a 10 billion well yeast IC plate then after just 1/3 of a day you would be able to know longevity had more than doubled, and based on well location, which chemicals were associated with the longevity heightening, and then make a completely new liposomal reaction 10 billion chemical library to screen on another plate, that screens about 30 billion new longevity chemicals each 24 hours or 1 trillion new longevity chemicals a month



Are buckycup liposomes possible

That's like 1 nm or less buckycup like electron wave concentrating semiconductor manufacturing chemical or nano technological reaction/assembly, integrated circuit technology geometries technology



Noting 1 nanometer dots are likely possible with 3nm integrated circuit feature technology with a more casual error rate, then a 300 mm wafer has about 9 times 10^12 one nanometer features, a 1 nm diameter well 5000 nm deep (5 micrometers) is a stack 5000 molecules deep and one molecule wide (carbon 120 picometers, about 9 carbons to a well width) that could be sequentially piled up in the well to a depth and volume of 45,000 molecules, this makes me think of things like, load it up, use radiation to initiate reaction or polymerization, or radiation to divide things like halogen atoms from their molecules to do new reactions, or load some areas with catalytic molecules like electrically addressable liquid crystal molecules with cobalt or Rh atoms on it to do chemistry, may also be cycled between a temperature with 1/2 the reaction rate and one with the full reaction rate to load it when cool, light and charge based reactions are also cool,



One use for this is to screen libraries of high utility chem



Doped si features at sides of each well could make sides of well electronegative or electropositive or provide a surface with spin polarization particularity, or have light emitting diode sides to place full circumference illumination at each well

Noting that photoactivated physiochemicals exist and are published at 2019, there are likely many better ones but a 20 separate colors of photons simultaneously or cumulative together at 1 microsecond or more rapidly, light responsive protein could be made, a 20 photon response accumulating version of polyrhodopsin could be made that changes the shape of the protein to a different shape with each amount of light energy, such as a photon, that is absorbed, the polyrhodopsin could be made to be white or transparent and support the continued whiteness of skin, the photoreceptor proteins could be part of physiochemical activation systems and body effecting systems that photoactivate at the interior of mouth and GI tract or body surface as people prefer



Longevity technology



Electrical engineering technology



Architect technology

Treon Verdery

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Oct 3, 2022, 10:40:29 AMOct 3
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I read human Eunuchs live 19 years longer than other people, plasma mass fractions and tissue homogenate chemicals from cow, horse, pig, or rat eunuchs as well as ordinary organisms could be compared to find chemical differences, then the different chemicals administered to c elegans to find out if any had longevizing or modulating effects, also noting an old rat and a young rat with connected circulatory systems causes greater rat youthfulness (as well as possibly longevity, I do not remember what it said) they could test eununch rats with their circulatory systems linked to ordinary rats of the same age to find out if there were longevity effects,



similarly there may be longevity chemicals sequential to the mTOR effects of rapamycin, published as making mice live 60% longer, connecting the circulatory systems of old rats that were, until 54 hours previously, on rapamycin, to those of old rats of the same age could find out if there were non mTOR longevization chemicals circulating at the previously rapamycin treated rats



The difference between the human age equivalent of 60 year old normal rats and 60 year old multimonths of previous rapamycin, rapamycin treated rats could be characterized as to their electrophoretic physiochemical difference, and the notably different chemicals tested on yeast and c elegans for longevity effects



The electrophoretic physiochemical difference between 30 and 40 year old human equivalent age rats and rapamycin treated 60 year old equivalent rats could be characterized, the most maintained physiochemicals from 30 year old to 60 year old rapamycin treated rats (as compared to 60 year old untreated rats) these physiochemicals could be tested on yeast and c elegans, if large mammals on rapamycin are utilized then that could be a plentiful source of chemicals to test mice or shrews with









The difference (a list of only which are shared) between all the chemical differences between young and old test mammals, and eununch test mammals could create a more rapidly testable list of chemicals that heighten longevity



Electrical engineering technology

Treon Verdery

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Oct 4, 2022, 4:12:26 AMOct 4
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Royal jelly is published as making mice live 25 (27%) longer, and queen bees live about 36 months, 12 to 24 times longer than other bees; noting that insect chemicals can cause mammals to live longer, it is possible other long lived insects could have chemicals that cause greater longevity. The mice that were longevized with royal jelly were fed it orally, bringing up the possibility of change to the proteins in it; some insects, queen termites, live 50 years, with some possibility of 100 years, feeding ground up insects to mice, both orally and as enteric capsule or coated material could find out if the ground up material of long lived insects cause greater longevity; another approach is to extract circulatory fluid (hemolymph) from these insects and find out if it makes yeast or c elegans or fish live longer, notably, fish as vertebrates could be notably suggestive of mammalian activity; another insect to screen for longevity chemicals are cicada nymphs (17 years), beetles of the genus Eleodes (up to 17 years)



longevity technology: I perceive I read playing back EEGs causes the brain to be better at learning and can reactivate emotions; it is possible that rather than EEG, electrical recordings from the surfaces of children and 16 year olds could be played back at the body surface to find out if it has any longevizing, wellness, or healthspan effects; this could be tested on nude mice, and might go well with the body and tissue thickness of mice as proof of concept; at humans something like a wireless recharging near body surface implant (kind of like a pacemaker) could provide the youthful EEG-like stimulus, notably it is possible that wave and frequency variants like nodal, antinodal or possibly depth-reaching electrical solitons https://phys.org/news/2006-05-solitons-electronics.html (dissipative solitons, 100 times farther travel than regular solitons) could be used; notably, some current levels and alternate voltages for the EEG-like frequencies could be used to possibly reach tissues at greater depth, as could wrist-to-wrist or leg to wrist, or even head to calf electrode placement



EEG playback technology: playing back EEGs to cause beneficial cognitive effects using different voltages or currents to reach deeper into tissue, notably though some currents and voltages might verge into tDCS (although AC-like) which is a different thing; the previously described gelatin capsule sized piezoelectric frond hair adhering and head skin seeking technology could also do some tDCS as well as EEG recording, playback and software based synthesis; children’s EEGs could also be played back at adults to find out if there were child-mind effects as well as, just possibly, longevising, wellness, or healthspan effects (seems unlikely, but is possible), notably children have more delta waves, and these occur during sleep, although less so at older people, these child EEG frequencies could be played back while adults sleep; this could be tested on rats, or some larger animal like cows, sheep, or horses



Longevity technology: Some biologically occuring tings do rapamycin like things https://www.ncbi.nlm.nih.gov/pubmed/29165314 It is possible that mutating these plants, then screening their chemicals on yeast and things like c elegans with 96 well plate technology could create much larger rapamycin like longevity chemicals at plants, these could then be genetically engineered into delicious as well as ubiquitous plant foods to create longevity producing fruits, grains, and food products like pasta, pizza, potato products, breading, there is also the possibility that moving these gnes and gene products from plants to eggs and milk could be beneficial



a molecular variant or rapamycin, rapamycin preassociated (attached) to a protein makes it 2000 times more active, “To probe the affinities involved in the formation of the FKBP.rapamycin.FRB complex, we used fluorescence polarization, surface plasmon resonance, and NMR spectroscopy. Analysis of the data shows that rapamycin binds to FRB with moderate affinity (K(d) = 26 +/- 0.8 microM). The FKBP12.rapamycin complex, however, binds to FRB 2000-fold more tightly (K(d) = 12 +/- 0.8 nM) than rapamycin alone. No interaction between FKBP and FRB was detected in the absence of rapamycin. These studies suggest that rapamycin's ability to bind to FRB, and by extension to mTOR, in the absence of FKBP is of little consequence under physiological conditions.” It is possible some molecular version of rapamycin (a rapalog) could cause mTOR activity without FKBP protein, FKBP has immunoeffects so that molecule could have less immunoeffects than rapamycin



Rapamycin that is more water soluble, “clinical development of its formulations was hampered due to its poor solubility and undesirable distribution in vivo. Chemical modification of rapamycin presents an opportunity for overcoming the obstacles and improving its therapeutic index. The objective of this study is to develop a drug-polymer conjugate to increase the solubility and cellular uptake of rapamycin.

METHODS:

We developed the rapamycin-polymer conjugate using a novel, linear, poly(ethylene glycol) (PEG) based multiblock copolymer. Cytotoxicity and cellular uptake of the rapamycin-polymer conjugate were evaluated in various cancer cells.

RESULTS:

The rapamycin-polymer conjugate provides enhanced solubility in water compared with free rapamycin and shows profound activity against a panel of human cancer cell lines. The rapamycin-polymer conjugate also presents high drug loading capacity (wt% ~ 26%) when GlyGlyGly is used as a linker. Cellular uptake of the conjugate was confirmed by confocal microscopic examination of PC-3 cells that were cultured in the presence of FITC-labled polymer (FITC-polymer).

CONCLUSION:

This study suggests that the rapamycin-polymer conjugate is a novel anti-cancer agent that may provide an attractive strategy for treatment of a wide variety of tumors.” hydrophilic rapamycin combined with regular rapamycin could reach a wider variety of tissue types, combined they could cause greater than the 60% longevity increase published at mice

Optically activated rapamycin, “We developed an optically activated rapamycin dimer” perhaps a topical benefit

focusing rapamycin effect on just one tissue (kidney), “Thus subcapsular delivery of rapamycin-loaded microspheres successfully inhibited local fibrotic response in UUO with less systemic effects. Therapeutic effect of released rapamycin was most prominent in close vicinity to the implanted microspheres.”

longevity technology: Liver function genes, like SNPs, could be a source of new longevity drugs, gene therapy or also germline gene modification, “To identify the pathways that could be responsible for rapamycin's longevity effect, we analyzed the transcriptome of liver from 25-month-old male and female mice fed rapamycin starting at 4 months of age. Few changes (<300 transcripts) were observed in transcriptome of rapamycin-fed males; however, a large number of transcripts (>4,500) changed significantly in females. Using multidimensional scaling and heatmap analyses, the male mice fed rapamycin were found to segregate into two groups: one group that is almost identical to control males (Rapa-1) and a second group (Rapa-2) that shows a change in gene expression (>4,000 transcripts) with more than 60% of the genes shared with female mice fed Rapa. Using ingenuity pathway analysis, 13 pathways were significantly altered in both Rapa-2 males and rapamycin-fed females with mitochondrial function as the most significantly changed pathway. Our findings show that rapamycin has a major effect on the transcriptome and point to several pathways that would likely impact the longevity.” They could do this analysis at a variety of tissues, including the gut, which I perceive I read secretes and might process a lot of physiochemicals

liposomes http://www.ijper.org/sites/default/files/IJPER_45_4_13.pdf

liposomes might or might not benefit rapamycin, a way to tell is if rapamycin has nootropic characteristics, possibly at a higher sample dose, and then see if a liposomal version causes more nootropic effect, notably though liposomes might cause longer plasma half life and could possibly have different peak plasma concentrations so that might effect a nootropic effect

about 76% of some actual rapamycin liposomes get turned into liposomes, with the rest at the remaining fluid, that suggests drinking the extra fluid, or eating the trehalose it might be dried out on could provide another simultaneous, possibly different tissue specific activity of rapamycin

depending on if phosphatidylcholine or phosphatidyl serine turns into, or is highly similar to phosphatidic acid liposomes made of these might be nonpreferred, suggesting a different liposomal production method “the efficacy of rapamycin is dependent on the level of phosphatidic acid (PA), which is required for the assembly of both mTORC1 and mTORC2 complexes. Rapamycin interacts with mTOR in a manner that is competitive with PA. Therefore, elevated levels of PA, which is common in cancer cells, increases the level of rapamycin needed to suppress both mTORC1 and mTORC2.”

liposome preservatives and how long liposomes last with refrigeration, “The use of cryoprotectants such as dextrose, sucrose, and trehalose may increase stability from hydrolysis. Also, samples may experience oxidation upon storage. The addition of small amounts of antioxidants during processing may stabilize the suspension and limit oxidation of the product. SUV should be stored above their transition temperature for no longer than ~24 hours. LUV may be store for a longer period of time if stored at 4-8°C when not in use. Hydrolysis of the lipid begins to occur immediately resulting in monoacyl derivatives (Lyso lipids) which act as detergents and disrupt the membrane, thus permeabilizing the membrane. After ~5-7 days at 4-8°C the internal contents will begin to leak indicating hydrolytic degradation of the lipid. If membrane structure is not a critical parameter in your experiments, vesicles may be stored for 1-2 months with minimal (<10%) hydrolytic degradation.”

distilled water is likely a way to make liposomes be longer lasting, “or by binding metal ions that initially induced aggregation. However, the presence of aggregation can accelerate the process of coalescence of liposomes”

ions at a solution could cause liposomes to last less long, that suggests when making liposomes drug and lecithin, or purified phosphatidylcholine, amounts at something like ultrasonication to make the most liposomes per volume, it is also possible filtering or centrifuging (?) liposomes could divide it from a fluid which might have uncombined, particularly oxidizable drug or phosphatidyl choline molecules

If there are longer versions of phosphatidylcholine they might make more durable liposomes, “Permeability and stability of liposomes are influenced by the rigidity/stiffness of the lipid bilayer.”

liposome size depends on how they are made, ultrasonication produced liposomes make “small unilamellar vesicles (SUV), 25 to 100 nm in size that consists of a single lipid bilayer.” shaking a liposome making solution with your arms makes “Large unilamellar vesicles (LUV), 100 to 400 nm in size that consists of a single lipid bilayer. Multilamellar vesicles (MLV), 200 nm to several microns that consist of two or more concentric bilayers.” it is possible a combination of all three types reaches a wider variety of tissues and likely has greater physiological activity and physiological availability than rapamycin at an enteric capsule

“liposomes prepared by using combinations of some lipids follows the order of physical stability form the correlation of the mean volume diameter, zeta potential and pH , egg lecithin (PC)/cholesterol (CH)/stearylamine (SA) < PC/CH/phosphatidylserine (PS) < bovine brain ceramides (CM)/CH/palmitic acid (PA)/CS < PC/CH/cholesteryl sulphate (CS) at 4°C, as well as at 25°C, 2122after a 6-month storage period” also, “Vesicles composed of saturated phospholipids were found more stable compared to phosphatidyl-choline(PC) liposomes”

Ethanol makes for better drug loading, “that rely on incubation temperatures above the phase transition temperature (Tc) of the bulk phospholipid to promote drug loading. In the absence of cholesterol, liposome permeability is enhanced at these temperatures which, in turn, can result in the collapse of the pH gradient and/or unstable loading. Doxorubicin loading studies, for example, indicate that the drug could not be loaded efficiently into cholesterol-free DSPC liposomes. this problem could be circumvented by the addition of ethanol as a permeabilityenhancer.Doxorubicinloadingratesincholesterol-free DSPC liposomes were 6.6-fold higher in the presence of ethanol. In addition, greater than 90% of the added doxorubicin was encapsulated within 2 h at 37 °C, an efficiency that was 2.3-fold greater than that observed in the absence of ethanol.” also, “Optimal ethanol concentrations ranged from 10% to 15% (v/v) and these concentrations did not significantly affect liposome size, retention.”

Dissolve in etoh first, then put the water in perhaps, perhaps some solvent that rapamycin is more soluble in than water could be put in the ultrasonic liposome maker at a 1 part per 100 amount to heighten colubility, another way is to see if the pile of rapamycin dissolves whn you stir the liposome making fluid

Liposomes can be dried over sorbitol, “formulation of proliposomes lipid dried over a fine particulate, water soluble support like sodium chloride, sorbitol or polysaccharides imparts adequate physical and chemical stability and are ideally suitable for lipophilic drugs” That might be functional with trehalose as well to absorb nonreacted fluids, while the trehalose might also preserve the liposomes

Trehalose as a liposome preservative, at lyophilized of liposomes, “aggregation of liposomes could be prevented by the formation of stable boundaries between the vesicles. The ability of cryoprotectants to form these stable boundaries has been attributed to their ability to replace the bound water around the bilayer via interaction with the polar region of the lipid 36head group (water replacement hypothesis).” also, as to things like trehalose, “high concentrations (up to 30 mol.% in some cases) are required” (to where it is gooey)

the trehalose, possibly, could be added to the fluid ahead of ultrasonication, “In the preferred embodiment, the liposomes are made in the presence of the combination of at least one sugar (sucrose, trehalose, lactose, maltose and glucose)”

liposomes could increase plasma half life of rapamycin availability

antioxidants as liposome preservatives, “use of antioxidants like α-tochopherols, betahydroxy toluene (BHT)”

Rapamycin is made from fungi, “Rapamycin and its analogs are clinically important macrolide compounds produced by Streptomyces hygroscopicus” so depending on how much rapamycin the fungi make those genes could be transferred to plants or perhaps mammals like humans

Rapamycin might last awhile at the body, there are three plasma half lifes I have read, .9 hours, 9 hours, and 56 hours, one thing online says, “The long elimination half-life of sirolimus necessitates a loading dose but allows once daily administration, which is more convenient”

Longevity technology: “Centenarians delay age-related methylation changes, and they can pass this methylation preservation ability on to their offspring.” suggests that drugs, or possibly some non-CRISPR CRISPR like thing could duplicate longevity methylation at everybody

a-tocopherol looks kind of like phenylethylamine, perhaps putting a nitrogen on it, and maybe trimming away a couple CH3s that are on the long alkane distal to the cylic (bicyclic) part, as well as putting a halogen atom on it (I do not know if 2 grams, at numerous doses of a-tocopherol is beneficial or nonbeneficial) so that just a few milligrams or even micrograms rather than 350 mg is a fun dose, also halogenation might outcompete a-tocopherol at some valuable body location, it could be a thing though, a stimulant that is perhaps slightly beneficial

IGF1 (Insulin like growth factor) as well as insulin if modified, perhaps with gene therapy or germline genetic modification could produce IGF1 as well as insulin that do sugar response things while being neutral to longevity, perhaps causing less AMPK activation (If that is what they do)

fetal environment as well as parental epigenetics are published as effecting longevity, methylation, ethylation and other epigenetic things could be modified with supplements or drugs during pregnancy to align the person the fetus becomes with highest longevity, wellness, and healthspan; I do not know if more methylation during pregnancy is the thing, or if just specif areas of DNA methylation are the thing, different tissues, at the devloping fetus have different epigentics,

How much food the parents eat makes a difference in progeny wellness, “There is consistent evidence from both mice and rat models that modulating the maternal dietary status such as protein restriction during pregnancy can significantly affect lifespan”, “F1 male mice exposed to maternal undernutrition during prenatal life produced F2 male offspring with impaired glucose tolerance and increased adiposity”, Also, at humans, “F2-generation offspring of women who were exposed to the famine of 1944–1945 in the Netherlands (i.e., Dutch Hunger Winter) throughout the gestational period had 1.8 times more health problems in their adulthood than descendants of non-exposed women [70]. The offspring of the fathers, but not the mothers who suffered from intrauterine undernutrition during the Dutch famine also had a significantly greater weight and body mass index in their adult life than the descendants of parents unexposed to the famine”, I perceive this suggests that at the fetus different amounts of something like methylation (also acetylation, phosphorylation, methylation, phosphorylation, sumoylation, and ubiquitination) could effect longevity, which suggests drug localization of methyl donors could be beneficial, perhaps optimal methylation of the CNS and heart could be a longevity area, “the epigenetic code changes dramatically during the embryonic development of the organism to initiate differential gene expression patterns between various developing tissues. This code consists of chemical modifications of DNA and histone proteins that play a crucial role in packing the DNA by forming nucleosomes.” Acetylation promotes gene expression, perhaps rather than methylation acetylation of longevity genes could cause greater longevity, also, “The dynamic “writing” and “erasing” of histone modifications are conducted by specific enzymes” suggesting that drugs that cause more beneficial enzymes to be produced, gene therapy as well as germline modification, particularly with tissue localization of beneficial amounts of enzymes could be beneficial, this could be a longevity techology; what one paper describes as socioeconomic adversity “In sons and grandsons of men born outside wedlock, a 1.64- and 1.83-fold excess risk of circulatory disease” Homesis also has an effect, so a drug or supplement that cause the right amount of stress, without the parents feeling the stress could be beneficial, “there is also evidence that exposure to mild stressors in early development can result in beneficial (hormetic) effects and that adaptive modulation of epigenetic processes could significantly contribute to these effects [92–94]. There is also evidence that adaptive/hormetic effects can persist over several generations”, as a longevity wellness drug, it is possible that parents could make their epigenetics (methylation, acetylation phosphorylation, others) like those of persons at the 99.999th percentile of longevity,

marmosets, a primate, could be used to measure the effect of epigenetics on longevity



finding longevity genes other than those that effect mTOR and AMPK: they could look at all the rodents (mice, beavers), and all the bats, and find the ones with the least and most favorable mTOR and AMPK genetics, then they could find the longest living rodents and bats with the least beneficial mTOR and AMPK genetics, it is possible that noting 33 year rodent lifespan difference the long lived non beneficial mTOR and AMPK rodents can be compared with the lifespan of rodents with highly favorable mTOR and AMPK genetics but lifespans of 1/2 to 1/4 or even 1/11 of that, then they can find the genes that cause the greater longevity even though mTOR and AMPK are least beneficial at the much longer lived rodents, also, they can just compare mice genetically modified to have highly beneficial mTOR and AMPK with the genes of beavers to find the beaver non-mTOR and non-AMPK genes that cause the greater longevity even though the beavers might have median mTOR and AMPK genetics; they can do the same thing with bats; The longevity drug benefit is that on finding the longevity genes of rodents that outlive rodents with optimal mTOR and AMPK genetics 4 to 11 times then the products of those genes, and the actual genes, can then be placed at mice to find out if they are longevity drugs and genes, characterizing humans as to the amount of the new longevity genes activity and the amounts of their gene products, is also beneficial.

crispr rodent gene swap, find the longevity gene swapping beaver and mouse genes with CRISPR/cas9 to find out which non mTOR and AMPK genes cause mice to live much longer like the 35 year beavers

Longevity technology:





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