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HELP: HPLC CHEMSTATION SOFTWARE

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Caobin Zhu

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Jul 4, 1997, 3:00:00 AM7/4/97
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Hi to all,
I have a strange situation. Recently I started to use HP HPLC
chemstation software. My supervisor lost the software manual and I am
not sure if we are using the software the right way. The problem is
that when I load a file back again which has been integrated before, I
LOST EVERY INTEGRATION DETAIL! This is terriable!
Any help would be greatly appreciated.
By the way, we are using Windows 95 with 16MB ram. the LC
chemstation software is rev. A.04.01.

Thanks.

helen_w.

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Jul 21, 1997, 3:00:00 AM7/21/97
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In article , cao...@flash.net says...
You do not have a problem, all HP chemstation versions save the chromatograms
separatly from the integration parameters. You must be sure to save your
methods (they contain the integration parameters).

Before you bring up a data file, the method you used to integrate should be
loaded first. In order to see everything, you need to reintegrate to see all
the details on the screen. Printing out hard copies as a reference is not a bad
idea. I have had too many computerized instruments crash and would have lost
all data if I didn't do that.

You should also contact HP - manuals are cheap (~$20 - $70).

Caobin Zhu

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Jul 28, 1997, 3:00:00 AM7/28/97
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On Fri, 04 Jul 1997 13:02:00 GMT, cao...@flash.net (Caobin Zhu)
wrote:

>Hi to all,
> I have a strange situation. Recently I started to use HP HPLC
>chemstation software. My supervisor lost the software manual and I am
>not sure if we are using the software the right way. The problem is
>that when I load a file back again which has been integrated before, I
>LOST EVERY INTEGRATION DETAIL! This is terriable!
> Any help would be greatly appreciated.
> By the way, we are using Windows 95 with 16MB ram. the LC
>chemstation software is rev. A.04.01.
>
>Thanks.


Hi to all,
Thanks for many peple's reply to my question. However I'd like
to make this question more specific (or clearer if it is not clear
last time and I am sorry for that if that is the case).

My problem is that AFTER I MADE EVERY MANUAL INTEGRATION TO
the LC chromatogram, and when I reload THAT FILE BACK, then ALL THAT
INTEGRATION IS LOST! I tried saving the method but still it does not
help. Any help would be greatly appreciated.
Thanks again.

Gary Radford

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Jul 30, 1997, 3:00:00 AM7/30/97
to
I'm assuming you are using HP['s current sotware for LC and from the
infomation that I had rec'd it IS supposed to save the manual
integration events....some people I work with do pcb's and are using an
older system Chemstation that you have to manually log the integration
events and then separately save this to a file yourself. I would expect
that your manual integrations are saved with the data file, not the
method file...? try looking in the data file directory and see if there
is a file wwith date of manual integration( different than the
acquisition time

Otherwise I would guess that there is either a sofware bug. or you may
need to save or turn on saving integration events

Does the online haelp give any reference to saving integration events?
Also just loading the data file may not be enough - you may also have to
load the manual integration events file

Well that's about all my guesses - I would try to find out from HP
software support centre if there is nothing in the online or CD help
files.
--


Gary Radford, Radford Scientific

The opinions expressed here represent the company I work for.

Cutting the salaries of the H? Board of Directors by 10% could result in
the hiring of 80 new support techs.....that would increase customer
satisfaction.( or maybe get the phone answered in under 10 minutes )

Gary Radford

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Aug 7, 1997, 3:00:00 AM8/7/97
to Scott Robertson

Scott Robertson wrote:
>
> Also something to consider is that in the new software you have to
> specifically select a command that indicates that you want to save
> manual integration events to the method. If you do this, and the method
> is loaded when you load the signal, the manual events you had previously
> saved will be applied to the loaded chromatogram. If you want more
> specifics, feel free to email me.
>
> Scott Robertson
> Anesta Corp.
>
> To reply via email, simply remove the NOSPAM.
Hi Scott

This doesn't make sense...that the manual events are saved with the
method and not the data file. If you are doing manual integration of a
complex chromatogram or crappy separation then each chromatogram is
probably going to need different baseline events and these should be
saved with the data file to later re-integrate if needed.

I could understand in a perfect world running standards or very similar
samples that the manual integration events could be saved with the
method and applied to a batch or sequence of data files.

If true this could be a major problem for anyone doing pcb or any other
mixed matrix or dirty samples at the lowest concentration levels.

Regards,

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