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How can we prepare a sodium acetate/citrate buffer (0,1 M, pH 6,0)

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Bert770

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Mar 29, 2004, 1:03:09 PM3/29/04
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I need to prepare a sodium acetate/citrate buffer (0,1 M, pH 6,0)for a
chromogenic substrate (ELISA). How much sodium acetate and how much
sodium citrate do I need to use to have a volume of 1L and a pH of
6,0? Thanks to anybody who will help me.

Roger Murphy

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Mar 30, 2004, 1:17:16 AM3/30/04
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Make a 0.1M solution of tri-sodium citrate and pH to 6.0 with 10% acetic
acid! or else go to your library and have a look at one of the many standard
buffer books.

"Bert770" <h.sa...@usherbrooke.ca> wrote in message
news:5dd58885.04032...@posting.google.com...

Bert770

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Mar 30, 2004, 8:09:05 AM3/30/04
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"Roger Murphy" <roger....@ludwig.edu.au> wrote in message news:<4069012c$1...@news.unimelb.edu.au>...

The buffer msut contain sodium acetate and sodium citrate not acetic
acid, it's not the same molecular weight! Any other suggestions? The
librairy of my faculty is quite poor in buffer books.

Florian Gembardt

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Mar 30, 2004, 12:40:16 PM3/30/04
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mw(na-acetate):82.03
for 0.1M you need 8.203g
mw(citric acid):192.1
for 0.1M you need 19.21g

for a ph6.0 you have to adjust the ph with acetic acid or naoh.

Bert770

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Mar 30, 2004, 10:30:21 PM3/30/04
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Florian Gembardt <pf...@web.de> wrote in message news:<c4cbe0$1kb$1...@brutus.eur.nl>...

Thanks but I also found this method:

"Substrate Buffer- Sodium Acetate Buffer pH 6.0

8.25g sodium acetate
Add 800ml of distilled water
Adjust to pH 6.0 with 0.1M citric acid (2.1g in 100ml distilled water)
Make up to 1000ml with distilled water
Aliquot into 100ml volumes and autoclave. "

Source: http://www.cabri.org/guidelines/animal/AHC9833521.html

Is that method also good?

I don't know if by sodium acetate/citrate buffer in the article they
mean, sodium acetate and sodium citrate or sodium acetate and citrate.

In the website, when they talk about sodium carbonate/bicarbonate,
they mean sodium carbonate and sodium bicarbonate, not sodium
carbonate and bicarbonate.
Any idea of what is the right answer?

Florian Gembardt

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Apr 7, 2004, 10:49:52 AM4/7/04
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you are talking about salts.salts consit of anions and cations.charge
should be zero.thats why it should be sodium bicarbonate.

srees...@gmail.com

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Jul 22, 2016, 5:11:50 AM7/22/16
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On Monday, March 29, 2004 at 11:33:09 PM UTC+5:30, Bert770 wrote:
> I need to prepare a sodium acetate buffer (0,1 M, pH 6,0)for
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