High Power Field?

[Sergei V. Gerasimov, MD]

Dear users of sci.bio.microbiology,

May anyone explain me what is the approximate volume of one high power
field (hpf). May I re-calculate the number of cells in 1 ml having to
say 5 cells per hpf?

Sergei

[Larry D. Farrell]

Depends completely on the depth of fluid in the field. I do not
understand your second sentence.

--
Larry D. Farrell, Ph.D.
Professor of Microbiology
Idaho State University

[d buebly]

On Sun, 29 Sep 2002 13:24:19 -0600, "Larry D. Farrell"
<farr...@isu.edu> wrote:

>"Sergei V. Gerasimov, MD" wrote:
>
>> Dear users of sci.bio.microbiology,
>>
>> May anyone explain me what is the approximate volume of one high power
>> field (hpf). May I re-calculate the number of cells in 1 ml having to
>> say 5 cells per hpf?
>>
>> Sergei
>
>Depends completely on the depth of fluid in the field. I do not
>understand your second sentence.

what is a high power field (hpf), is there a standardization of
microscope magnifications?
or is it an area measurement, something like nanometers squared?

1 hpf= 4400 nm^2 ????

[Bob]

On Sun, 29 Sep 2002 21:47:06 +0300, "Sergei V. Gerasimov, MD"
<serg...@mail.lviv.ua> wrote:

>This is a multi-part message in MIME format.
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If you are using a calibrated chamber, such as a Petroff-Hauser
chamber, then the depth is defined by the construction of the device,
and the conversion is given in the instructions.

If you are not using a chamber calibrated for this purpose, I would be
reluctant to try the conversion. If approx vales are adequate, maybe
you can include an internal standard of some beads.

bob