SBSC Flow Cytometry Working Group

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Welcome to the SBSC Flow Cytometry Working Group discussion list.

This is a working group within the Synthetic Biology Standards Consortium (SBSC).

NOTE: All posts and replies to this group are publicly viewable. Only members may post to this group.

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Marc Salit, Jake Beal2

3/9/17

NIST and SBSC engagement with the Flow Cytometry Working Group project

Thank you to Marc and the rest of the team for your help and support, which has gotten us to the edge

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NIST and SBSC engagement with the Flow Cytometry Working Group project

Thank you to Marc and the rest of the team for your help and support, which has gotten us to the edge

3/9/17

Ross, David J. (Fed)

3/6/17

sony instrument

http://www.sonybiotechnology.com/sp6800/

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sony instrument

http://www.sonybiotechnology.com/sp6800/

3/6/17

Jake Beal, Ariel Haim Hecht4

2/28/17

Scatter plots of data

Hi Jake, Great, thanks. Btw, forgot to send it yesterday, and thanks Dave for the plug, if

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Scatter plots of data

Hi Jake, Great, thanks. Btw, forgot to send it yesterday, and thanks Dave for the plug, if

2/28/17

Jake Beal

2/27/17

Initial interlab analysis is looking great!

Hello, all: I've performed an initial analysis, which contains all of the data except for Rice

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Initial interlab analysis is looking great!

Hello, all: I've performed an initial analysis, which contains all of the data except for Rice

2/27/17

Nicholas DeLateur, … John Sexton24

2/26/17

Feb Interlab samples

MIT data is uploaded. On Fri, Feb 24, 2017 at 9:18 PM, John Sexton <john.t...@rice.edu>

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Feb Interlab samples

MIT data is uploaded. On Fri, Feb 24, 2017 at 9:18 PM, John Sexton <john.t...@rice.edu>

2/26/17

Jake Beal, John Sexton2

2/15/17

Experimental procedure in new document

Thanks Jake. I attempted to reorganize the Google Drive a little, but feel free to change things up

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Experimental procedure in new document

Thanks Jake. I attempted to reorganize the Google Drive a little, but feel free to change things up

2/15/17

Jake Beal, … Ross, David J. (Fed)12

2/9/17

Follow-on from today's meeting

Excellent! Please everybody make sure that Nicholas has the correct shipping information to send you

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Follow-on from today's meeting

Excellent! Please everybody make sure that Nicholas has the correct shipping information to send you

2/9/17

Jake Beal, … John Sexton5

1/30/17

Followups on yesterday's call

Thanks Sarah. In skimming that document briefly, I thought it was interesting to see that "

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Followups on yesterday's call

Thanks Sarah. In skimming that document briefly, I thought it was interesting to see that "

1/30/17

Nicholas DeLateur, … Ariel Haim Hecht7

1/27/17

Initial Freeze Thaw Procedure Validation

I don't know, hence why I did both, and am eminently interested in learning more how to better

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Initial Freeze Thaw Procedure Validation

I don't know, hence why I did both, and am eminently interested in learning more how to better

1/27/17

Ross, David J. (Fed), … Jake Beal11

1/23/17

Data uploaded

Thank you, Nicholas; I will look forward to the full report tomorrow! Thanks, -Jake On Mon, Jan 23,

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Data uploaded

Thank you, Nicholas; I will look forward to the full report tomorrow! Thanks, -Jake On Mon, Jan 23,

1/23/17

Brian Teague

1/23/17

The MIT contingent won't be around today

We're at an SBC-wide symposium. We'll be back next week! Brian & Nicholas -- Brian Teague

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The MIT contingent won't be around today

We're at an SBC-wide symposium. We'll be back next week! Brian & Nicholas -- Brian Teague

1/23/17

Nicholas DeLateur

1/9/17

Flow Cytometer Maintenance

The company is called Cytek Our contact there is Ray Lannigan (rlan...@cytekbio.com) "Their

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Flow Cytometer Maintenance

The company is called Cytek Our contact there is Ray Lannigan (rlan...@cytekbio.com) "Their

1/9/17

John Sexton, Jake Beal2

1/9/17

First Pass at Data Analysis

Adding to John's work, my own TASBE-based analysis shows we've got pretty good agreement

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First Pass at Data Analysis

Adding to John's work, my own TASBE-based analysis shows we've got pretty good agreement

1/9/17

John Sexton, … Nicholas DeLateur8

1/9/17

Documenting Bead Lots in Experiment Descriptions

Sebastian documented the lot number in his FlowCal files. Specifically, for both his "20160918

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Documenting Bead Lots in Experiment Descriptions

Sebastian documented the lot number in his FlowCal files. Specifically, for both his "20160918

1/9/17

Ariel Haim Hecht, Jake Beal3

1/5/17

NIST Stanford data collected, will be uploaded shortly

Thanks Jake. The data is up and ready to roll. I've included a readme sheet in the folder in case

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NIST Stanford data collected, will be uploaded shortly

Thanks Jake. The data is up and ready to roll. I've included a readme sheet in the folder in case

1/5/17

Ross, David J. (Fed), Jake Beal3

1/3/17

NIST Gaithersburg data uploaded

Hi, David: I've done a first pass with the data that you submitted, and ran into some related

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NIST Gaithersburg data uploaded

Hi, David: I've done a first pass with the data that you submitted, and ran into some related

1/3/17

Ross, David J. (Fed), … Jake Beal13

12/17/16

Some technical notes

A note of reminder about focus: this conversation is drifting far away from the measurement of

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Some technical notes

A note of reminder about focus: this conversation is drifting far away from the measurement of

12/17/16

Brian Teague, … Jake Beal4

12/14/16

Jake: would you write out the "five scenarios" for me?

Sure; I've added it with the name "Driving scenarios, measurand, and target precision"

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Jake: would you write out the "five scenarios" for me?

Sure; I've added it with the name "Driving scenarios, measurand, and target precision"

12/14/16

Ross, David J. (Fed), … Ariel Haim Hecht7

12/10/16

Preliminary data

We're mixing our own M9. Pre-mixed was out of stock when we went shopping. From stories I've

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Preliminary data

We're mixing our own M9. Pre-mixed was out of stock when we went shopping. From stories I've

12/10/16

Ariel Haim Hecht, … Ross, David J. (Fed)7

12/9/16

Proposing hypotheses and designing experiments

From Paul DeRose (Fluorescence Guy at NIST) in response to my question on whether there are

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Proposing hypotheses and designing experiments

From Paul DeRose (Fluorescence Guy at NIST) in response to my question on whether there are

12/9/16

John Sexton, … Jake Beal8

12/6/16

Minor Post-Meeting Tasks

Provided by SpheroTech. Thanks, -Jake (sent from my phone) On Dec 6, 2016 5:26 PM, "Ariel Haim

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Minor Post-Meeting Tasks

Provided by SpheroTech. Thanks, -Jake (sent from my phone) On Dec 6, 2016 5:26 PM, "Ariel Haim

12/6/16

jakebeal

12/5/16

Interlab planning slides

Hi, folks: Enclosed please find my current slides with interlab plan sketches, for discussion this

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Interlab planning slides

Hi, folks: Enclosed please find my current slides with interlab plan sketches, for discussion this

12/5/16

Jake Beal, … Sebastián Castillo Hair11

12/2/16

Doodle poll: flow cytometry meeting

First, thanks to Marc for meeting with us. We really appreciate your perspective on these issues. I

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Doodle poll: flow cytometry meeting

First, thanks to Marc for meeting with us. We really appreciate your perspective on these issues. I

12/2/16

jakebeal, Peter McLean2

11/21/16

Units without spectra

The followup is awesome. Straight to my questions/comments: - I'm concerned about the unit

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Units without spectra

The followup is awesome. Straight to my questions/comments: - I'm concerned about the unit

11/21/16

John Sexton, … Sebastián Castillo Hair3

11/7/16

High-Res Bead Spectrum Data

I did some basic analysis on John's bead data and produced the following plot: This is showing

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High-Res Bead Spectrum Data

I did some basic analysis on John's bead data and produced the following plot: This is showing

11/7/16

Peter McLean, Brian Teague3

11/7/16

Addressing the inter-channel problem

On 11/02/2016 04:52 PM, Peter McLean wrote: > > I have added a `pfm` folder into the analysis

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Addressing the inter-channel problem

On 11/02/2016 04:52 PM, Peter McLean wrote: > > I have added a `pfm` folder into the analysis

11/7/16

Peter McLean

11/3/16

Updates to our draft documentation

I've updated, added to, and rearranged some of the documentation in our publications directory.

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Updates to our draft documentation

I've updated, added to, and rearranged some of the documentation in our publications directory.

11/3/16

Peter McLean, Jake Beal3

10/12/16

Spectral datasets for Rainbow Beads and Fluorophores

So for a formal followup, the 2016 interlab folder has been reorganized a bit, but has all of the

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Spectral datasets for Rainbow Beads and Fluorophores

So for a formal followup, the 2016 interlab folder has been reorganized a bit, but has all of the

10/12/16

Peter McLean, … jakebeal5

7/28/16

Joining the FlowCyt Fun!

How did I manage to fail on saying the day? Here's what it should have said: Several of us have

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Joining the FlowCyt Fun!

How did I manage to fail on saying the day? Here's what it should have said: Several of us have

7/28/16

jakebeal

1/5/16

Request for input: Minimum Flow Cytometry Unit Information

Hello, all: We are now making the first limited dissemination of the draft MinFlowUnit standard (

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Request for input: Minimum Flow Cytometry Unit Information

Hello, all: We are now making the first limited dissemination of the draft MinFlowUnit standard (

1/5/16