Hi everyone,
I have performed an analysis in the non-alignment based-form. I would like to know the number of reads that mapped against the transcriptome, but I have some doubts about the output:
I know that my sample has 23.005.930 reads in each library (PE-reads), and the log file says:
...[info] Counted 14891682 total reads in the equivalence classes
...[info] Mapping rate = 71.4513%
Is it correct to say 14.891.682 are the number of reads from each library that are actually used to quantify, and 71.45% of them have mapped against my transcriptome? So I have about 10 million of PE-reads mapped against the transcriptome?
Thank you so much for your help,
Lucila.