Hello! I'm a graduate student trying to get a handle on different quantification methods for RNAseq data. I've successfully built sailfish indices for my transcriptome, but I'm running into problems when I try and use sailfish quant.
The command I'm running is the following
sailfish quant -i <path to index> -l "T=PE:O=><:S=U" -1 left -2 right -o <path to output>
in the above command "left" and "right" refer to files containing a list of files of reads, as seen below.
less left
tissue_1_R1.fastq
tissue_2_R1.fastq
tissue_3_R1.fastq
however, when I run sailfish quant, I get the following error message.
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Exception : [boost::bad_any_cast: failed conversion using boost:any_cast]
================
sailfish quant was invoked improperly.
For usage information, try sailfish quant --help
For reference, I'm using the older version (0.6.3), because I'm working on a shared computer cluster, and they haven't updated.
My question is this- is the error message I'm receiving above due to me improperly inputting sailfish commands, or is this something I need to take up with my Sysadmins on the cluster, to update Sailfish and look into the Boost errors?
Thanks in advance,
Robert