enzyme catalysis

[Deepa Maheshvare]

Dear All,

I would like to find out whether the subunit of an enzyme or the enzyme complex is involved in catalyzing each of the reactions present in glycolysis  pathway.

Can we find this information on SABIO?

Thanks a lot for your time and kind consideration.

Deepa

[Maja Rey]

Dear Deepa,
in general you can't filter for this kind of information. Sometimes in the variant field (wildtype/mutant) of the single entry there is further information given about which part or subunit of the enzyme has been used in the experiment.
Kind regards,
Maja

[Deepa Maheshvare]

Dear Maja,

Thanks for the clarification. Reactions catalysed by wildtype is of my interest.

For example, if we consider the protein present in SABIO EntryID: 1569

Under the Modifiers tag ,the following information is given,

Modifiers
name	location	effect	comment	protein complex
glucokinase(Enzyme)	-	Modifier-Catalyst	-	P35557;

 

And for the protein with with SABIO EntryID: 12712,we have the following

Modifiers
name	location	effect	comment	protein complex
phosphopyruvate hydratase(Enzyme)	-	Modifier-Catalyst	-	(P13929)*2;

 

 

From the above would it be right if I infer the following:

1.The reaction in the first example is catalysed by 1 subunit of protein

2.The reaction in the second example is catalysed by the protein complex, which is made of two subunits of the protein.

 

Please correct me if my interpretation is wrong.

Thank you,

Deepa

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[Maja Rey]

Dear Deepa,

in your examples you can only see that the protein P13929 is active as a dimer naturally and the protein P35557 is active as a monomer. This information is taken from the publication or from UniProtKB directly. Some of the kinetic data in SABIO-RK are derived from experiments where only subunits or parts of enzymes have been used. This information is, as I mentioned before, included in the variant field (wildtype/mutant) within the General Information as in SABIO EntryIDs 42083 or 56012.

Kind Regards,

Maja