rsem-simulate-read does not produce any fastq files.
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Marzie E. Rasekh
Sep 20, 2023, 1:24:19 AM9/20/23
to RSEM Users
When I run simulate-read, I get the fasta file, sim.genes.results, and sim.isoforms.results but no fastq files. I am not getting any error message. Just says number of resimulation is 0.
Refs.loadRefs finished!
GEN 1000000
GEN 2000000
GEN 3000000
GEN 4000000
GEN 5000000
GEN 6000000
GEN 7000000
GEN 8000000
GEN 9000000
GEN 10000000
GEN 11000000
GEN 12000000
GEN 13000000
GEN 14000000
GEN 15000000
GEN 16000000
GEN 17000000
GEN 18000000
GEN 19000000
GEN 20000000
GEN 21000000
GEN 22000000
GEN 23000000
GEN 24000000
Total number of resimulation is 0
Here is the output:
$ rsem-simulate-reads Mus_musculus.GRCm38.93/rsem/GRCm38 GRCm38/rsem/SRR5511184.stat/SRR5511184.model GRCm38/rsem/SRR5511184.isoforms.results 0.22 24906633 simulated/RSEM/sim1_SRR5511184 --seed 0
Refs.loadRefs finished!
GEN 1000000
GEN 2000000
GEN 3000000
GEN 4000000
GEN 5000000
GEN 6000000
GEN 7000000
GEN 8000000
GEN 9000000
GEN 10000000
GEN 11000000
GEN 12000000
GEN 13000000
GEN 14000000
GEN 15000000
GEN 16000000
GEN 17000000
GEN 18000000
GEN 19000000
GEN 20000000
GEN 21000000
GEN 22000000
GEN 23000000
GEN 24000000
Total number of resimulation is 0
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