RSEM run Error: --append-names" failed!

[Fathmath]

Hi,

I am trying to run RSEM. It was running successfully until I got this error:

 --append-names" failed! Please check if you provide correct parameters/options for the pipeline!

I used this command to run it:

rsem-calculate-expression \
  --paired-end \
  --bam \
  --estimate-rspd \
  --append-names \
  --num-threads 8 \
  /mnt/c/Users/path to file/sorted.bam \
  /mnt/c/Users/path to file/Sp1_reference \
  /mnt/c/Users/path to file/rsem_output

Temp output files were generated and it processed about 49 million reads.

Please help.

Thank you

[projectNew23]

Append name is not an important tag. You can remove that and run then check. As append name help you in adding a specific name to all the generated data. So it is negligible when we know what the data is.

Hope it helped you