rsem-calculate-expression -p 1 \
--bowtie2-path /Users/yvonnezhao/miniconda/bin \
It first proceeds but then stopped with the error message:
[E::hts_open_format] Failed to open file "b" : No such file or directory
Assertion failed: (in != 0), function BamWriter, file ./BamWriter.h, line 54.
"rsem-run-em /Users/yvonnezhao/RSEM_tutorial/ref/mouse_ref 3 exp/LPS_6h exp/LPS_6h.temp/LPS_6h exp/LPS_6h.stat/LPS_6h -p 1 -b b exp/LPS_6h.temp/LPS_6h.bam 0 --append-names" failed! Plase check if you provide correct parameters/options for the pipeline!
Could you help me understand which part went wrong and how to fix it?
RSEM website: http://deweylab.biostat.wisc.edu/rsem/
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