rssem runs and build index and run-em also shows logs (n reads processed...). But after that it shows like -
rsem-run-em
cDNA_hap12 1
path/AP7290021
path/AP7290021.temp/AP7290021
path/AP7290021.stat/AP7290021 -p 20 -b
path/AP7290021.temp/AP7290021.bam 0 " failed! Plase check if you provide correct parameters/options for the pipeline!
"Exited with exit code 255."
Command I gave -
rsem-calculate-expression -p 20 --bowtie2 <read_path>/AP7290021-singles-trim.fq ./cDNA_hap12/cDNA_hap12 <output_path>/AP7290021
Please suggest a solution soon.