Error Message: Strand is neither '+' nor '-'

[hongye zhou]

.gtf file might be corrupted!

Stop at line : scaffold_0       exon    LTR/Copia/uwas  1278560 1280947 .       +       .       gene_id "uwas_1_754.1"; transcript_id "lala";

Error Message: Strand is neither '+' nor '-'!

As seen, this gtf file contains strand information, so what is the problem?

[Bo Li]

Hi Hongye,

Can you check if there are extra [tab] between adjacent fields?

P.S., according to http://mblab.wustl.edu/GTF22.html, it seems the order
of your second and third fields are not correct?

Best,
Bo

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