Error Using RSEM Prepare Reference

[Christopher Bryan]

Command:

rsem-prepare-reference --gtf index/gencode.v23lift37.annotation.gtf \
--star  index/GRCh37.primary_assembly.genome.fa \
/ref/human

Output:

Parsed 200000 lines
Parsed 400000 lines
Parsed 600000 lines
Parsed 800000 lines
Parsed 1000000 lines
Parsed 1200000 lines
Parsed 1400000 lines
Parsed 1600000 lines
Parsed 1800000 lines
Parsed 2000000 lines
Parsed 2200000 lines
Parsed 2400000 lines
Parsing gtf File is done!
index/GRCh37.primary_assembly.genome.fa is processed!
198356 transcripts are extracted.
Extracting sequences is done!
Group File is generated!
Transcript Information File is generated!
Chromosome List File is generated!
Extracted Sequences File is generated!

rsem-preref /ref/human.transcripts.fa 1 /ref/human
Cannot open /ref/human.transcripts.fa! It may not exist.
"rsem-preref /ref/human.transcripts.fa 1 /ref/human" failed! Plase check if you provide correct parameters/options for the pipeline!

I attempted to create a reference for the tutorial dataset using the commands on Github, but I received the same message.

[Manuel Moradiellos Corpus]

Were you able to solve this issue??

Because I'm running with the same issue and I still don't understand why...

Checking other posts I've seen that some people had problems doing latter steps as they used a different name for the '/ref/human' argument when running other functions, but in our case I'm not sure what's wrong

Thanks in advance