What are acceptable results in rsem-plot-model?

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Dekel Wainer

Jul 15, 2020, 3:50:45 AM7/15/20
to RSEM Users
Hi all!
I performed rsem-calculate-expression on 4 samples and received what seems to me low-quality alignment. 
Where do you usually place the limit and say that a sample is too low quality to continue analysis? Is there something I can change in the run?

Here are 2/4 samples' alignment statistics:
1. Unique: 26%; Multi: 41%; Unalignable: 33%
2. Unique: 20%; Multi: 31%; Unalignable: 49%

Thanks a lot!
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