inconsistent read length!

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JiYeon Han

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Jan 1, 2018, 6:24:12 AM1/1/18

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Hello i'm newbie to sequencing analysis.

I recently tried to solve this problem but couldn't get the right result.

the warning sign was like this :

Paired-end read HWI-D00574:287:CBPNWANXX:4:2205:5975:94929 has alignments with inconsistent mate lengths!

"rsem-parse-alignments 00.Reference/rsem_ref 30.RSEM/SKMEL1.temp/SKMEL1 30.RSEM/SKMEL1.stat/SKMEL1 20.ubu/SKMEL1.transcriptome_alignments_filtered.bam 3 -tag XM" failed! Plase check if you provide correct parameters/options for the pipeline!

/opt/gridengine/default/spool/multi-0-3/job_scripts/123583: line 63: =: command not found

Is there anybody who knows how to fix this problem..?

Yu Wang

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Jun 8, 2021, 11:25:15 PM6/8/21

to RSEM Users

Hi, all

Read A00632:98:HC27MDRXY:1:2102:2799:1047 has alignments with inconsistent read lengths!

Did anyone have idea about this error? Thanks in advanced.

Best,

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