QTL analysis on F3 single plants

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Itay

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Apr 13, 2022, 3:05:59 PM4/13/22

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Hello Dr. Broman and group

Due to some failures with growing F2 I want to try different approach for using rqtl. I am thinking on taking 2 F3 seeds from each F2 plants, plant them in total of 500 plants and conduct the qtl analysis by genotyping and phenotyping all 500 single plants. What do you think? Am I going to far with my thoughts or whether it is feasible?

What are the consequences of it?

Karl Broman

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Apr 14, 2022, 12:06:57 PM4/14/22

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Genotyping and phenotyping the F3 plants seems a perfectly reasonable strategy, though I don't have any particular suggestions or experience with this.