I have not used the rPi capture software, but I did do a simple arduino capture for 16mm film with a stepper drive and without using a trigger.
I have twice captured several reels, with over 1 kilometer of film, forget how many frames and there was no issue with framing and no trigger, apart from the usual jitter from slightly worn sprocket holes.
I did use two trigger/limit switches on the film going to the take up reel as I did not have a good slipping clutch.