Ets1 Download For Pc ##VERIFIED##
Arnou Vargo
The c-Ets1 protein is closely related to c-Ets2. It is believed that these two proteins derived from the same ancestor gene by duplication [9, 10]. The genomes of Drosophila and sea urchin contain only one Ets1-related gene, D-ets2 (Pointed) and SU-ets2, respectively, whereas birds, reptiles, amphibians and mammals harbor both the ets1 and ets2 genes [11, 12]. In humans, the ets1 and ets2 genes are located on two distinct chromosomes, ets1 on chromosome #11, ets2 on chromosome #21 [7]. No ets1-related gene has been found in the genome of the Caenorhabditis elegans, although this nematode worm does express a variety of other Ets proteins [13].
In birds and reptiles, the c-ets1 locus contains two different start sites leading to the expression of p68c-ets1 and p54c-ets1. These two proteins differ in their N-terminal sequence [14]. Exons α (I) and β (II) code for the N-terminus of p68c-ets1, whereas exon I54 (A) encodes the N-terminus of p54c-ets1 (Fig. 1). The exon β domain of p68c-ets1 shows a high degree of homology to the N-terminus of c-Ets2 and was thus called the Ets1-beta/Ets2-conserved sequence (BEC). In mammals, only exon I54 is present, therefore, p68c-ets1 is not expressed.
The human TATA-less ets1 gene contains eight exons (A, III-IX) [15]. Transcripts either harbor all exons or lack exon IV- or exon VII- or exon IV/VII-specific sequence [16]. Only two proteins are generated from these RNAs, p54c-ets1 (full length Ets1) and p42c-ets1 (ΔVII-Ets1) (Fig. 1). The Ets1 protein sequence is highly conserved among species. E.g., the DNA and protein sequence of chicken Ets1 is 85% or 95%, respectively, identical to the corresponding sequence of human Ets1 [17]. The Ets1 protein can be divided into six domains, A-F (Fig. 1). The E-domain is the DNA binding Ets-domain. The adjoining D- (or exon VII-) domain and the F-domain are regulatory domains that control the activity of the E-domain. The A- und B- domains also function as regulatory units, whereas the C-domain is the activation domain of p54c-ets1 and p42c-ets1.
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