Question

[Mengjia Fu]

Hi Alex,

Thanks a lot your  support to all STAR users.

I am very interested in plant  N6-Methyladenosine RNA Modification, Recently I aligned the input （control data）and IP sample（Sequencing data obtained after m6A antibody enrichment）to reference genome by STAR respectively,  found that the Uniquely mapped reads % only 15.06%  in the IP.Log.final.out ,and other IP samples ' Uniquely mapped reads %  up to 45% ； In input .Log.final.out this value is more than 70%，Do you think the Uniquely mapped reads % only 15.06% in IP.Log.final.out   is correct or do you recommend other value for this parameter?

[Alexander Dobin]

Hi,

I am not familiar with this technology, so cannot really comment on what the acceptable mapped % is.

Generally, 15% is a low %.