--limitGenomeGenerateRAM option seems using only the default value (31GB)

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ERA

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Jan 3, 2017, 10:16:19 PM1/3/17

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Hi,

Whatever the value I assigned to –limitGenomeGenerateRAM, STAR seems using only the default value (31G) for this option as indicated in the error message and the Log.out files. I failed indexing a huge genome (17Gb). The machine I used have higher than 512G of RAM though.

These are my trials and error messages I got,

Trial #1: --limitGenomeGenerateRAM 110000000000 (110G) for – --genomeChrBinNbits 12

Error message #1: EXITING because of FATAL PARAMETER ERROR: limitGenomeGenerateRAM=31000000000is too small for your genome. SOLUTION: please specify limitGenomeGenerateRAM not less than105603022208 and make that much RAM available

I reduced values for each of these options: 106G and 10; 38G and 8; 37G and 7; and finally 36GB and 4 but I had the same problem.

Trial #5: --limitGenomeGenerateRAM 36000000000 (36G) for – --genomeChrBinNbits 4

Error message #5: EXITING because of FATAL PARAMETER ERROR: limitGenomeGenerateRAM=31000000000 is too small for your genome. SOLUTION: please specify limitGenomeGenerateRAM not less than35742657706 and make that much RAM available.

Any help resolving would be greatly appreciated,

Thanks a lot

Alexander Dobin

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Jan 6, 2017, 4:22:38 PM1/6/17

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Hi @ERA

please send me the Log.out file of of the trial 1.

Cheers

Alex

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