Hello,
In my previous run with redundans that included all the three steps: reduction, scaffolding and gapclosure. I found that this assembly didn't include the mate pair libraires because they didn't pass the QC during the scaffolding step. So, as suggested in the manual I modified the "is" file and reran the redundans on the contigs.reduced.fa file with --noreduction and got the following errors, can you please help me troubleshoot it, Thanks!
python2.7 /redundans-dev/redundans.py -i trim36*.fastq MK-HMTI_*.fastq.int_trimmed -f ./Hmisstrim36_reddev/contigs.reduced.fa -t 20 --log Hmiss_nored-dev.log -o ./HN_noreddev --noreduction
[WARNING] Poor quality: Major orientation (FR) represent 85.11% of pairs in trim36_PE100_500bp_R1.fastq - trim36_PE100_500bp_R2.fastq: [106, 8511, 1294, 89]
[WARNING] Poor quality: Major orientation (FR) represent 86.1% of pairs in trim36_PE100_500bp_R1.fastq - trim36_PE100_500bp_R2.fastq: [96, 8610, 1221, 73]
Traceback (most recent call last):
File "/redundans-dev/redundans.py", line 517, in <module>
main()
File "/redundans-dev/redundans.py", line 512, in main
o.norearrangements, o.verbose, o.log)
File "/redundans-dev/redundans.py", line 321, in redundans
identity, overlap, minLength, resume)
File "/redundans-dev/redundans.py", line 146, in run_scaffolding
iters=1, resume=resume, verbose=0, log=log, basename=basename)
File "/redundans-dev/redundans.py", line 243, in run_gapclosing
stats = FastaIndex(pout).stats()
File "/redundans-dev/bin/FastaIndex.py", line 341, in stats
longest = max(stats[0] for stats in self.id2stats.itervalues())
ValueError: max() arg is an empty sequence