emission strength

[Sheema R]

Hi Steve,

I have a couple of questions. 

1) In Rapidstorm's localization file, is the column corresponding to "emission strength" amplitude of the gaussian model, "I0=I/2*pi*simgax*sigmay", or is it "I"?

2) Also, would you please clarify if the unit for emission strength is A/D or photons? In the localization file, it is written emission strength is in A/D, but in your response to Zuzka on 4/7/14 you mention that "The intensity is the signal intensity (without background) in photons." 

I appreciate your help.

Thanks,

Sheema

[Steve Wolter]

Hi Sheema, +Zuzka Q. because I lied earlier,

On Mon, Nov 20, 2017 at 3:56 PM Sheema R <shee...@gmail.com> wrote:

Hi Steve,

I have a couple of questions. 

1) In Rapidstorm's localization file, is the column corresponding to "emission strength" amplitude of the gaussian model, "I0=I/2*pi*simgax*sigmay", or is it "I"?

It is I (the integral under the Gaussian).

 

2) Also, would you please clarify if the unit for emission strength is A/D or photons? In the localization file, it is written emission strength is in A/D, but in your response to Zuzka on 4/7/14 you mention that "The intensity is the signal intensity (without background) in photons." 

I've looked it up, and my memory served me badly. Current code says A/D counts: https://github.com/stevewolter/rapidSTORM/blob/aada50739181ec8555a5cf2c6831a90f33a468d4/guf/LocalizationCreator.cpp#L67

Sorry about the confusion.

Best, Steve

 

I appreciate your help.

Thanks,

Sheema

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[Sheema R]

Thanks Steve for your quick response, it is very helpful!

-Sheema

On Wednesday, November 22, 2017 at 1:35:00 PM UTC-5, Steve Wolter wrote:

Hi Sheema, +Zuzka Q. because I lied earlier,

On Mon, Nov 20, 2017 at 3:56 PM Sheema R <shee...@gmail.com> wrote:

Hi Steve,

I have a couple of questions. 

1) In Rapidstorm's localization file, is the column corresponding to "emission strength" amplitude of the gaussian model, "I0=I/2*pi*simgax*sigmay", or is it "I"?

It is I (the integral under the Gaussian).

 

2) Also, would you please clarify if the unit for emission strength is A/D or photons? In the localization file, it is written emission strength is in A/D, but in your response to Zuzka on 4/7/14 you mention that "The intensity is the signal intensity (without background) in photons." 

I've looked it up, and my memory served me badly. Current code says A/D counts: https://github.com/stevewolter/rapidSTORM/blob/aada50739181ec8555a5cf2c6831a90f33a468d4/guf/LocalizationCreator.cpp#L67

Sorry about the confusion.

Best, Steve

 

I appreciate your help.

Thanks,

Sheema

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