STORM.png from sets of frames

[mdan...@asu.edu]

Dear Steve,

I have the following problem: My idea was to get a Movie where the STORM-image is developing. Therefore I need a STORM image form all my 5000 frames, from 4999 frames, from 4998 frames and so on - in order to sum them up to a movie which can be run on a presentation simultaneously with the raw data.
The idea was to choose 5000 for "Size of one slice in images" and 1 for "Start new slice every n frames" - so that the program takes first all 0-5000 frames, than 1-5000, 2-5000,...,since there are not more than 5000 frames available. rapidSTORM does not give me an error for this, but my computer shuts down.

Do you know how I can manage this?

Thank you!

Melanie

[Steve Wolter]

Hi Melanie,

sorry for the very late answer, I've been preoccupied with my day job, wedding planning and my PhD defense. I think the problem is that you're looking at a massive need for memory: For the last localization, 5000 result images need to be kept in memory and potentially displayed. I assume that you already disabled the "Display image" checkbox in the viewer, but I suspect that it still won't be enough.

Three workarounds come to mind:

1. Reduce the output image's size by cropping (set minposx, minposy, maxposx and maxposy in the expression filter) and using larger pixel sizes.

2. Use a "Localization file" output so that you can write the localizations to 5000 different files and then use a batch script to convert each into an image.

3. Use a larger slice distance and run the job multiple times with different values for the first image to process.

I hope this helps, either you if you still have the problem, or a future reader.

Best regards, Steve

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