rapidSTORM error - Read error at scanline

[Pedro Vallejo]

Dear all,

I've been getting this error while reconstructing some recent data sets (see attached image of error message). Has anyone encountered a similar error? If so, can you share with me where it comes from and what I can do to address it?

Thank you,

Best,

Pedro

[Sven Proppert]

Dear Pedro,

this is a very often encountered error but nothing you need to worry about too much. It simply means that your data-acquisition software (I assume you use Andor Solis?) had some problems writing the last frames of your .tif measurement. You can easily check this by, e.g., loading a "corrupted" file into fiji. If I am right, fiji will tell you, that the file consists of, e.g., 14998 frames while it should have been 15000 frames.

There are some ways to bypass this error - they all just skip the last frames of the measurement:
1) set an expression filter like this: frame < 14990 fr (would skip the last 10 frames of a 15000 frames measurement. make sure to always add the unit fr!!!)
2) you could also set a value for maxframe (change "expression to assign from" in an expression dropdown field to "maxframe" and then enter 14990 fr to the input field below)
3) directly underneath the input field where you load the tif-files is a checkbox called something like "maximum number of frames" (don't remember the exact label but it shoud be easy to find). Check this box and enter an appropriate value like 14990. be aware that you must not add fr here, because this field has the unit attached automatically. - if I remember correctly
Be aware, that you only need one of the three solutions. Whichever suits you best...

If you have problems finding the fields I indicated above, you might need to switch from beginner- to expert mode.

Let us know, if that helps you. If not, I can also provide you with some screenshots.

Best,
Sven