Rapid Typing 4.6

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RapidTypingis a convenient and easy-to-use keyboard trainer that will help you improve your typing speed and reduce typos. With its lessons organized for various student level, RapidTyping will teach you touch typing or enhance existing skills.

Typing tutor can be used both in the classroom under the guidance of teacher, as well as for self-study. Available export the training statistics in the different formats and creating your own training courses.

Advance your typing speed and accuracy with the newest typing tutor! With Rapid Typing Tutor you will learn using your keyboard more efficiently in just a few easy lessons for absolutely free. Kids can learn by playing a typing game, while adults can take pre-configured courses or create their own training lessons. Rapid Typing Tutor is perfect for adults and kids, students and teachers, and provides comprehensive reporting and progress tracking for every student. Powerful statistics include 15 different parameters, including words-per-minute, characters-per-minute and accuracy reports.

The innovative typing tutor has a cheerful and intuitive user interface full of colors to make learning to type fun. The typing tutor features a variety of visual cues to help adults and kids master the computer keyboard, and provides full course statistics in tables and charts. Supporting multiple users makes Rapid Typing Tutor usable at schools, colleges and universities.

Secretaries, teachers and writers will find the ability of Rapid Typing Tutor to create their own courses custom-tailored to each student truly indispensible. Custom courses make it possible creating your own typing tutor meeting your own requirements. Whether you need to master a specific key, set of keys or a keyboard layout that you need to master, a custom-built typing tutor is your best solution.

The free typing tutor includes a full virtual keyboard with both hands moving over it to display the proper typing position for each hand and finger. Highlighted zones for each finger make placing your fingers correctly a second nature in no time.

For non-standard layout users, Rapid Typing Tutor automatically creates a new virtual keyboard based on the layout. You can take typing lessons for multiple keyboard layouts by quickly switching between them.

The beginner course helps to learn the position of keys on the keyboard. After completing this course, you will know which finger to use to hit each key without looking at the keyboard. The course also covers upper-case letters, special symbols, and the numeric pad.

You can evaluate your own performance or that of your kids or students at any time by simply looking at charts. Rapid Typing Tutor tracks your progress, and allows you to view your results at any time.

The editor shows the number of words and symbols in a lesson and the ratio between letters and the TAB and ENTER special keys. The editor filters out symbols that are missing on the existing keyboard from the imported text (such as special Word symbols or foreign-language symbols).

Make Your Own Plan Plan your lessons according to your skills and capabilities. Rapid Typing Tutor offers you complete freedom of learning, allowing you to make your own lessons and plan your studies. No need to force yourself into a Procrustean bed of pre-defined learning plans!

Typing Tutor is not just another boring keyboard training tool. With numerous colorful themes and avatars and a game-like environment, Typing Tutor makes learning typing fun for both adults and children. A built-in typing game makes learning to type an easy and entertaining experience.

Touch typing improves typing speed and accuracy. A touch typist never looks at the keyboard. The fingers hit the right keys by force of habit. The typist is entirely focused on the text being typed, reading the words and phrases as the fingers type the text reflexively. Touch typing can be learned by performing special exercises.

Stage one of the process begins with learning the home row of the keyboard (the row beginning with the Caps Lock key). Looking at the keyboard is strictly forbidden. This is followed by learning the lower and upper rows, the numbers row, upper-case letters and special symbols.

Be patient and practice as often as possible. Despite the simple Touch typing technique, it takes some time to gain high speed. By following the rules, you will see your speed and accuracy improve over time.

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The opportunistic human pathogen Propionibacterium acnes is composed of a number of distinct phylogroups, designated types IA1, IA2, IB, IC, II, and III, which vary in their production of putative virulence factors, their inflammatory potential, and their biochemical, aggregative, and morphological characteristics. Although multilocus sequence typing (MLST) currently represents the gold standard for unambiguous phylogroup classification and individual strain identification, it is a labor-intensive and time-consuming technique. As a consequence, we developed a multiplex touchdown PCR assay that in a single reaction can confirm the species identity and phylogeny of an isolate based on its pattern of reaction with six primer sets that target the 16S rRNA gene (all isolates), ATPase (types IA1, IA2, and IC), sodA (types IA2 and IB), atpD (type II), and recA (type III) housekeeping genes, as well as a Fic family toxin gene (type IC). When applied to 312 P. acnes isolates previously characterized by MLST and representing types IA1 (n=145), IA2 (n=20), IB (n=65), IC (n=7), II (n=45), and III (n=30), the multiplex displayed 100% sensitivity and 100% specificity for detecting isolates within each targeted phylogroup. No cross-reactivity with isolates from other bacterial species was observed. This multiplex assay will provide researchers with a rapid, high-throughput, and technically undemanding typing method for epidemiological and phylogenetic investigations. It will facilitate studies investigating the association of lineages with various infections and clinical conditions, and it will serve as a prescreening tool to maximize the number of genetically diverse isolates selected for downstream higher-resolution sequence-based analyses.

Introduction. Outbreaks of carbapenem-resistant A. baumannii and A. nosocomialis have occurred worldwide in healthcare settings. Rapid and reliable molecular typing of bacterial isolates is vital for the effective surveillance of institutional outbreaks. The Pan-PCR and OXA-PCR assays are two multiplex PCR-based assays for the molecular typing of Acinetobacter species.

Methodology. A total of 105 carbapenem-resistant A. baumannii isolates (CRABs) and 93 carbapenem-resistant A. nosocomialis isolates (CRANs) obtained from blood cultures were used for molecular typing by the Pan-PCR and OXA-PCR assays and two multilocus sequence typing (MLST) schemes.

Human respiratory syncytial virus (HRSV) is the leading pathogens causing acute respiratory infections (ARI) in children under five years old. We aimed to investigate the distribution of HRSV subtypes and explore the relationship between viral subtypes and clinical symptoms and disease severity.

HRSV is the main causative virus of bronchopneumonia in infants and children. The multiplex one-step qRT-PCR not only provides a rapid and effective diagnosis of HRSV infection, but also allows its typing. There were no significant differences in the severity of HRSV infection between subtypes A and B, except significant gender-specific and age-specific differences in some clinical characteristics and laboratory results. Knowing the viral load of HRSV infection can help understanding the clinical features of different subtypes of HRSV infection.

Therefore, we aimed to detect HRSV infections, identify virus subtypes and analyze viral load of hospitalized children with ARI from November 2016 to April 2017 in Zhejiang Province by multiplex One-Step qRT-PCR. We also explored the correlation between different subtypes and viral load of HRSV and clinical characteristics as well as severity of the disease. This study will help improve the prevention, control and treatment of HRSV in Zhejiang Province, which can help to reduce both patient hospitalization time and its associated financial costs.

Patients were diagnosed with ARI carrying respiratory symptoms (such as cough, rhinorrea, nasal congestion, sneezing) and signs of lower respiratory tract involvement (respiratory distress, crackles, wheezing, or chest-X ray infiltrates). Patients were diagnosed with neumonia carrying crackles or respiratory distress and X-ray pneumonic infiltrates, and bronchopneumonia carrying wheezing or respiratory distress.

The reference strains used in this study were Long strains for HRSV subtype A and CH1856 for HRSV subtype B. Both strains were purchased from the China Center for Type Culture Collection and have been stored in our Central Laboratory, Children's Hospital, Zhejiang University School of Medicine, for many years. The recombinant positive plasmid containing HRSV M gene was provided by Shanghai Sangon Biotech Co., Ltd.

Serum IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ levels were determined using a CBA HumanTh1/Th2 Cytokine Kit II (BD Biosciences, San Diego, CA, USA) according to the manufacturer's specifications. Following the acquisition of sample data using a FACScalibur flow cytometer (BD Biosciences), results were generated using BD CBA Software (BD Biosciences, San Jose, CA, USA). Then we established the standard curve for each reagent. 1.0 pg/mL was the lowest detection limit for these six cytokines, while 5000 pg/mL was the highest.

T-cell subsets were detected by multicolor flow cytometry (FACSCalibur, BD, USA) using blood samples containing heparin. Mouse anti-human CD45-FITC, CD3-PC5, CD4-PE, and CD8-ECD monoclonal antibodies, and other reagents were purchased from BD. Data was analyzed by the MultiTEST software.

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