Another excellent PALM algorithm
Ricardo Henriques
Gene Expression and Biophysics Unit
Institute of Molecular Medicine
Faculty of Medicine, University of Lisbon Av. Prof. Egas Moniz
1649-028 Lisbon, Portugal
Phone: + 351 217999503,
Ext: 47318
Fax: + 351 217999504
E-mail: rhenr...@fm.ul.pt
~~ Or ~~
Unité de Biochimie Structurale et Cellulaire
Département de Biologie Structurale et Chimie
Institut Pasteur
25 rue du Docteur Roux
75724 Paris Cedex 15
Phone: +33 1 40 61 31 70
Ext: 3170
E-mail: rica...@pasteur.fr
Ricardo Henriques
Software site - http://code.google.com/p/palm3d/
Publication - http://dx.doi.org/10.1038/nmeth.1571
Cheers,
R
On Feb 21, 10:50 am, Ricardo Henriques <paxca...@gmail.com> wrote:
> Hello everyone,
> Andrew York from Hari Shroff's lab recently released also an excellent open-source algorithm for 3D PALM, check it here or the publication here.
> Cheers,
> Ricardo
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Ricardo Henriques
>
> Gene Expression and Biophysics Unit
> Institute of Molecular Medicine
> Faculty of Medicine, University of Lisbon Av. Prof. Egas Moniz
> 1649-028 Lisbon, Portugal
> Phone: + 351 217999503,
> Ext: 47318
> Fax: + 351 217999504
> Unité de Biochimie Structurale et Cellulaire
> Département de Biologie Structurale et Chimie
> Institut Pasteur
> 25 rue du Docteur Roux
> 75724 Paris Cedex 15
> Phone: +33 1 40 61 31 70
> Ext: 3170
Ann Wheeler
I have a question about exactly how Quickpalm works, I'm not a phycist
by training so I apologise if this was discussed in your paper and I've
not quite understood it.
I've been setting up Palm imaging on a microscope system very similar to
yours. However I've been using biological specimens. I have already
corrected the images for drift and attach the results. As you can see
from the dataset attached I was trying to take some images of Eos-Actin.
Admittedly I didn't take perhaps as many frames as I should as the Andor
software was being fussy about data export but I'm still a bit confused
why the actin, which is in a filament, comes out dotty after analysis?
When I've looked at samples with punctate staining they look just fine.
Can I ask does Quickpalm work by looking for individual PSFs? If so when
you will have overlapping PSFs, such as in the actin filaments in my
sample, how does Quickpalm deal with that?
Thanks in advance for your advice.
Ann
Dr Ann Wheeler
Blizard Advanced Light Microscopy Facility Manager
Blizard Institute of Cell and Molecular Science
Barts and The London School of Medicine and Dentistry
Queen Mary University of London
Blizard Building
4 Newark Street
London E1 2AT
Phone: 020 7882 2406
Fax: 020 7882 2180
On 21/02/2011 09:58, Ricardo Henriques wrote:
> Ups, seams the links didn't go through. Here they are:
> Software site - http://code.google.com/p/palm3d/
> Publication - http://dx.doi.org/10.1038/nmeth.1571
>
> Cheers,
> R
>
> On Feb 21, 10:50 am, Ricardo Henriques<paxca...@gmail.com> wrote:
>> Hello everyone,
>> Andrew York from Hari Shroff's lab recently released also an excellent open-source algorithm for 3D PALM, check it here or the publication here.
>> Cheers,
>> Ricardo
>>
>> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>> Ricardo Henriques
>>
>> Gene Expression and Biophysics Unit
>> Institute of Molecular Medicine
>> Faculty of Medicine, University of Lisbon Av. Prof. Egas Moniz
>> 1649-028 Lisbon, Portugal
>> Phone: + 351 217999503,
>> Ext: 47318
>> Fax: + 351 217999504
>> E-mail: rhenriq...@fm.ul.pt
>> ~~ Or ~~
>> Unit� de Biochimie Structurale et Cellulaire
>> D�partement de Biologie Structurale et Chimie
Ricardo Henriques
Unité de Biochimie Structurale et Cellulaire Département de Biologie Structurale et Chimie
Institut Pasteur 25 rue du Docteur Roux 75724 Paris Cedex 15 Phone: +33 1 40 61 31 70
Ext: 3170
E-mail: ricar...@pasteur.fr
--
Ricardo Henriques
PhD Student
Gene Expression and Biophysics Unit
Institute of Molecular Medicine
Faculty of Medicine, University of Lisbon
Av. Prof. Egas Moniz
1649-028 Lisbon, Portugal
Phone: + 351 217999503, Ext: 47318
Fax: + 351 217999504