[quickpalm] Dataset example - sub-diffraction beads under astigmatism for 3D PALM or STORM

[Ricardo Henriques]

Hi everyone,

I have just uploaded a small dataset example of how beads change their width/height over depth when imaged with our cylindrical lens (referenced on the paper).

You can use it to see if your system is giving out a similar performance or to test the "Create 3D calibration" method on the QuickPALM plugin, I've attached a snapshot of the calibration table graph generated with the dataset.

The image has 106.67nm pixel size and each slide was taken with a 10nm step, the link to the dataset: http://quickpalm.googlegroups.com/web/Subdiffraction+beads+under+astigmatism+voxel+106x106x10nm.tif

You may also want to check:

- the tutorial of how to create a 3D calibration table: http://code.google.com/p/quickpalm/wiki/Tutorial_Create_3D_calibration_table

- the thread on the characteristics of the cylindrical lens for astigmatism: http://groups.google.com/group/quickpalm/t/e0f7b5bb9d816df4

Cheers,

Ricardo Henriques

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Ricardo Henriques

PhD Student Gene Expression and Biophysics Unit  

Institute of Molecular Medicine

Faculty of Medicine, University of Lisbon Av. Prof. Egas Moniz 

1649-028 Lisbon, Portugal 

Phone: + 351 217999503, 

Ext: 47318 

Fax: + 351 217999504 

E-mail: rhenr...@fm.ul.pt

~~ Or ~~ 

PhD Student (in collaboration with) Groupe Imagerie et Modélisation
(Computational Imaging & Modeling Group)
Département Biologie Cellulaire et Infections
CNRS URA 2582
Institut Pasteur
25-28 rue du Docteur Roux
75015 Paris, France

Phone: +33 1 40 61 31 70

Ext: 3170

E-mail: rica...@pasteur.fr