Hi, Divya Thakur.
If you do not have an hourly dataset, then just fill the average data in the left-most column (12:00 AM).
For running the QUAL2K and create a model, you just need to input the river water quality data in the "Headwater" worksheet, which appoints the beginning of the river segment. Other water quality data to build a model should be in "Point Source" and "Diffuse Source", which are the water quality of the inflow to the river segment you are studying.
The WQ Data worksheet and other yellow worksheets are used for calibrating the model, not for creating a model.
If you fill in the WQ data worksheet, the black square dot will appear in the model (graph). The dots show the observational data which the model should follow in order to be properly calibrated. To adjust the model for following the dots, you should make some (usually a lot) of trial-and-error in changing the model coefficients.