FW: Problem with Reference Sprauer: Please Help

[Roy, Arindam]

Hello All,

 

Please see the message below from Brett. Have anyone of you seen this?

 

Brett, Unfortunately I have not seen this with my instrument. Do you think 20 psi is too high? If you keep it lets say at 10 psi, do you see the same?

David, What do you think about this?

 

Thanks

Roy

 

B_Wenner <bwe...@verizon.net> wrote:  

 

 Hello Roy,

I have recently experienced an issue with the reference sprayer, and I
was wondering if you or other Agilent Q-TOF users have experienced the
same. This past week the reference sprayer has started giving a
unstable spray. That is, periodically (every few seconds) the spray
seems to drop out, resulting in a downward spike in the TIC. I know
it is the reference sprayer because if I plot an EIC of a reference
ion, it exhibits this drop-out behavior, but an EIC of a background
ion coming from the analytical sprayer does not. Furthermore, if I
remove the reference nebulizer from the electrospray chamber and allow
it to spray on a black surface, I can physically see the dot of
moisture come and go. The nebulizing pressure does not fluctuate (20
psi). I have done a thorough investigation and tightening of all the
connections to the valves associated with pneumatic delivery of the
reference/cal solutions. I cannot find a leak anywhere. If you are
aware of anyone else experiencing this issue please let me know. I'm
at a loss. Thanks.

Best Regards,
Brett Wenner
Duke University


On Nov 8, 9:09 am, royar...@yahoo.com wrote:
Hello all,

Dr. David Weil from Agilent is thinking about doing another QTOF up
date (Web demo) on Tuesday of next week and he is wanting to ask the
QTOF users group what questions we might have about the use of the
1GHZ (the current system we all have) system and software?
Dr. Weil is also going to review the new 4GHZ system and mention
results from the Universal IRM source.

So if you have any questions/ideas regarding the QTOF, that you would
 like Dr. Weil to discuss during his web demo, then please send those
 to david_w...@agilent.com or simply reply to this e mail.

Best regards,

Roy

P.S. As always if I can share/facilitate anything about QTOF, please let me know. Has any of you found any bug in the software?

[B_Wenner]

Yes, I've tried varying the nebulizer pressure anywere between 5-30
psi. Although the fluctuations are somewhat attenuated at 5-10 psi,
the problem still exists. Also, if I disconnect the PEEK tubing from
the nebulizer and watch a drop of reference solution accumulate on the
end of the tubing, I have convinced myself that I can see an
occasional tiny bubble exit the tubing. To me this would indicate a
leak, but as I mentioned I cannot find one anywhere.

Brett

On Nov 9, 11:47 am, "Roy, Arindam" <Arindam....@Covidien.com> wrote:
> Hello All,
>
> Please see the message below from Brett. Have anyone of you seen this?
>
> Brett, Unfortunately I have not seen this with my instrument. Do you
> think 20 psi is too high? If you keep it lets say at 10 psi, do you see
> the same?
> David, What do you think about this?
>
> Thanks
> Roy
>

[david...@agilent.com]

To you all..

For the QTOF the Reference Sprayer MUST be set at 5 psi and not above that.

You might want to check to make sure you don't have a leak in the line also that the head pressure in the bottle is correct.

David Weil

[Greer, Ronda (OWE)]

Hi Roy,

 

Hope things are going good for you in St Louis.  Thanks for spearheading the QTOF group.  Just to let you know, I’ll be out of the lab on Tuesday morning,  but will be in on Tuesday afternoon.

 

Thanks,

Ronda

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[Roy, Arindam]

Hi Ronda,

 

Its good to hear from you. Hope all is well with QTOF and Photography! St Louis is good from yesterday because its 67.F. now!! Yeay!

 

Brett,

 

We would be very interested to know your experience of using the reference sprayer at 5 psi. My sprayer has been set at 5 psi (which I have changed in the past to 10 psi at most), and never had any problem. If you still see the problem, you may may have to call Agilent. Never the less please keep us updated.

 

David,

 

Once you have finalized the session, would you please let us know the time , date and other "instructions" regarding the session?

 

Best,

Roy

---

From: QT...@googlegroups.com [mailto:QT...@googlegroups.com] On Behalf Of Greer, Ronda (OWE)
Sent: Monday, November 12, 2007 8:49 AM
To: QT...@googlegroups.com
Subject: RE: Problem with Reference Sprauer: Please Help

[david...@agilent.com]

The QTOF presentation is going to be rescheduled from this coming Tuesday to a date in December.

I was not able to get all the information together for the TC in time

 

Please do submit questions/concerns that you would like addressed in the future.

 

Regards

 

David Weil

[B_Wenner]

This morning Jim Stricklin, our Agilent service engineer stopped by,
and we spent some time diaganosing the reference sprayer issue. We
decided flush the valve and lines associated with the reference
solution with a higher rate (1.5 mL/min) of mobile phase to better
visualize if a leak was present. To do this, we first unscrewed/
removed the bottle of reference solution and placed a beaker under
the
dip tube. Then we disconnected the PEEK tubing from the reference
nubulizer and connected the tubing directly the HPLC pump. From the
tune page, we selected the box for reference solution bottle A to
switch the valve to the open position. We then pumped acetonitrile
from the HPLC at 1.5 mL/min. After about 20 seconds we observed an
obvious leak around the nut that connected the reference solution dip
tube to the valve. This leak was not visible with the low flow rate
at which the reference solution is delivered under normal orperating
conditions. Upon removing the nut, it was obvious that it mas not
properly seated in the valve port. After a quick cleaning of the
threads and reseating/tightening of the nut, everthing seems to be
fine. Long story short, my initial suspicion of a leak was correct.
All is good now. Thaks for all or your help.

Brett

[Roy, Arindam]

Thanks Brett for keeping us in the loop. Great job! I am glad the problem is fixed.

Best,

Roy

P.S. Please don’t forget to send your suggestions (I am sure you have) for next webX demo which will be conducted by David Weil in Dec, 07 !!!

-----Original Message-----
From: QT...@googlegroups.com [mailto:QT...@googlegroups.com] On Behalf Of B_Wenner
Sent: Tuesday, November 13, 2007 1:45 PM
To: QTOF