Video cues require a target, which must be a file containing video. While QLab can play files in any format supported by AVFoundation, the following formats are recommended , listed in order of preference, for videos without transparency:
Prores 422 Proxy
Prores 422 LT
PhotoJPG
H.264
For videos with transparency, also referred to as alpha channel support, AVFoundation only supports ProRes 4444.