I am currently facing a small problem with taxonomy assignments of certain OTUs.
I have run QIIME 1 on amplicon sequencing data, and performed de novo OTU picking using 97% identity threshold, and subsequently assigned taxonomy using greengenes_release/gg_13_8_otus version.
I was puzzled about the assinment of OTUs to the species Streptococcus alactolyiticus, thus I have recovered the corresponding OTUs and performed BLASTn search, revealing that most of the OTUs actually belong to the species S. thermophilus, which is consistent with the analysed sample.
Is there a way to correct the taxonomic assignment in .biom file?
Thank you in advance for your kind reply.