join_paired_ends.py -f $TMPDIR/bioc3101_2016_read1.fastq -r $TMPDIR/bioc3101_2016_read2.fastq -b $TMPDIR/bioc3101_2016_barcodes.fastq -o $TMPDIR/fastq-join_joined
Traceback (most recent call last): File "/imports/home0/sejj036/qiime191/bin/join_paired_ends.py", line 178, in <module> main() File "/imports/home0/sejj036/qiime191/bin/join_paired_ends.py", line 174, in main write_synced_barcodes_fastq(assembly_fp, index_reads) File "/imports/home0/sejj036/qiime191/lib/python2.7/site-packages/qiime/join_paired_ends.py", line 68, in write_synced_barcodes_fastq " paired-end ID processed was:\n\'%s\'\n" % (joined_label))StopIteration:
Reached end of index-reads file before iterating through joined paired-end-reads file! Except for missing paired-end reads that did not survive assembly, your index and paired-end reads files must be in the same order! Also, check that the index-reads and paired-end reads have identical headers. The last joined paired-end ID processed was:'M01520:130:000000000-ALUEB:1:2106:16170:1516 1:N:0:0'
[sejj036@login09 761977.1.]$ head bioc3101_2016_read1.fastq@M01520:130:000000000-ALUEB:1:2106:15023:1418 1:N:0:0TACAGAGGGTGCTAGCGTTGTTCGGTTTCATTGGGCGTACAGTTCGTGTAGGCGTTTTTTTCTGTCATTTGTTATATCCCTCGTCTCTACCTTGGTTCGTCGCTTTTTACTGGCTTGCTTTATTTCCTTCTTTTTGTGTTGTATTCCCTGTTTATCGTTTTCATGCGTATCTATCTGGATGCTCTCCTTTGGCTTTGGCTTCTTCCTTGTTTGTTTCTGTCTCTGTTTCTCTTTTTCTTGTTTATCAATC+AAAAA111AA1A1A1FEEEEFEE0000B12BDD10AAE///222A/A/BF11AA//AF/>E012B22B2@2B0222B@2@B>//?F022>111111/1//<///?/11/<F21/00/?@111>@22212111111/00?0/01??1<111>1=111.0>..10=00.<...=0=000000//0000/<0;0/:/./0///./0009;00;0///;/0;00;0;C00;000;000009/9///-9//////@M01520:130:000000000-ALUEB:1:2106:14763:1432 1:N:0:0TACGTAGGGGTCTAGCGTTTCTCTTTTTCACTGTGCGTTTTTTTTTCTTATTCTTTTTTTTTTTTCTTTTTTTTTCTCCTTTTTCTCTTCTCCTTTTCTTCCTTTTTTTCTTTTTTTCTTTTTTTCTTTATTTTTTTTTTTTTTTTCTTTTTTATTTTTTTATTTCTTATCTATTTTTTTTTACTCCTTTTTCTTTTTCTTCTTTCTTTTCCTTTTCTTTCTCTTTTTTTCTTTTTCTTTTTTATCTCTC+>1>111>1A11>BG3F1AE01BF3331B02AFDD1ABB0//////A01D22222D111>>E//>E01>B1EE?//0B>11121?0?222>>1111211<<<1?1?11>-0<1111-</==D00;:/0000:0:00---:-----9-////BF>-//9/:---/;;//9/B/B/99///-9;--//////9/9BB//9//9:/9///9/9/////9//9///9/9///:--/////9//9/9/;-9/////@M01520:130:000000000-ALUEB:1:2106:16060:1443 1:N:0:0TACGAAGGACCCGAGCGTTATCCGGATTTATTGGGTTTATAGGTTGCGTAGGCGGCGCGGTACGTCGGGTGTGATATCTCGGTGCTTATCTCCGTATCTTCTTTCGCTACTTCCGTGCTTGAGGTCTGGAGTGGTGACTGGTATTTTCTGTGTAGCGGTGTACTGCGTAGCTATCGTATGTATGTCCAGTGTCTTATTCGGGTCTCTGGCCCGTTCCTGTCTCTTTGGCTCTTTTTTCTGTGTAGCATTC
[sejj036@login09 761977.1.]$ head bioc3101_2016_read2.fastq@M01520:130:000000000-ALUEB:1:2106:15023:1418 3:N:0:0CCTTTTTTCTCCCCTCTCTTTCTCTTTTCTTCTTCTTTATCTTTCCTTTTTGTCTCCTTCTCCTCTGTTTTTCCTCCCTATATCTCCGCATTTCCCCTCTACTCCTTTTTTTCCACTCCCCTCTTTCTTCCTCTTGCTTGCCTTTTTCTTTCTTCTTTCCTCTTTTTTGCCTTTGTCTTTCTCTCTTGTCTTTTCTTTCCTCCTACCTGCCCTTTACTCCCAGTCTTTTCTACCCTCGTTTTCACCCTCT+>1>>>D@1BD3B1A111BBFGF3A333DD13A2AA2AB2D2222AA122A1/A22AABEF1AA10112D1BA0BE10/01B1DAF2@///??D221>0?F112@@11211>>>B2>100?/<?F1122B21B>F1111B1/B11B<B<1>1221@21@2@1111>@1//11111111=>=<111111111<<1100000000/<00.00...;::0000/./0:000:00:000.0.-....000099./@M01520:130:000000000-ALUEB:1:2106:14763:1432 3:N:0:0CCTTTTTTCTCCCCTCTCTTTCTCTTCTCTTCTTCTTTTTCTTTCCTTTTTTCCTCCTTCTCCTCTTTTTTTCTTCCTAATATCTACTCTTTTCTCCTCTCCTCTCTCCTTTCCTCTTTCCTCTCCCTTTCTCCTTTCTTCCTTTATCCTTTTCTTTTCTTTTTTTTTTCTCTTTTTTTTCTCCTCTTCCTTTTTTTTTCTTCTTCTCTCCCTTTCCTCTCTTTTTTTCTTTCCTTCTCTTTCTCTCTCT+1111>DD>>F@@AA111BBEGDE333ADA3D12AA2AB11011A2112BB1A01DA0BABB1A1A111AAA>>1E21121D2DBF2@B12BBED121B>G0010101101B@@1111112>B1F0000111B21112>B12112B1BB122212111B211>1<<<</-01111111-<@/0000/:;00::000----/=00;00;000///;90//////;///99/////////////;/;///;//@M01520:130:000000000-ALUEB:1:2106:16060:1443 3:N:0:0CCCGTTTTCTCCCCTGTCCTTCTTGCCTCTGCGTCAGGAACTGTCCTGTGACCCGCCTTCTCCTCTGGTCTTCCTTTCGTTATCTACGCATTTCACCGCTCCTCCGTTATTTCCATTCTCCTCTCCTTTCCTCCATCTCGGTTGTTTCGATTTCTGTTTCGTATTTTTTCTCCGTTATTTCTCACCCTCCTTACCTCTCCTCCTTCGCACTCTTTCTTCCCTTTATCTCCTTCTTTCTCTTGTTTCCTTC
[sejj036@login09 761977.1.]$ head bioc3101_2016_barcodes.fastq@M01520:130:000000000-ALUEB:1:2106:15023:1418 2:N:0:0TACTTGTCTGAT+>1111111311@@M01520:130:000000000-ALUEB:1:2106:14763:1432 2:N:0:0TTTCACCAGGTT+>1111B1111@1@M01520:130:000000000-ALUEB:1:2106:16060:1443 2:N:0:0TTTCACTAGGTG
[sejj036@login09 761977.1.]$ tail bioc3101_2016_read1.fastq+AAAA1DC?F11B1BGFGGGCCAGEEG?E1B1DDFGHGGGGHH1E/AAEAEGG1EE?/>//>EFHHH2B11>FFFGHFH1>/</?FGDBGH210?>?CCHHH/<//??2FGHF11<?/AF?CFFDD111F<F.EG./...=CC@:C0C00;FGGFBF@GCAEFGFGGGGFGGGGGG90B/ABBFF-BB-9@@FF@@9@FE9@@-9-AEFFFF/;//9/BFBF/BFFBB?--:A---AB--9--;-;?A99A@M01520:130:000000000-ALUEB:1:1109:18959:28767 1:N:0:0TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGCGCGTAGGCGGCTAGGTAAGTCAGTGGTGAAATCTCCCAGCTTAACTCGGAAACTGCCATTGATACTATTTATCTTGAATACTTTGGAGGTAAGCGGAATTTGTCATGTAGCGGTGAAATGCTTAGATATGACATAGAACACCAATTGCGAAGGCAGCTTACTATACAGTTATTGACACTGAGGCACGAAAGCGTGGGGATCAAAC+AAAA11BAC11B3B1FFGG1AAFEEG0E1D2DFFGGEGGGHH1B//AAAEEF1EG///00BBFGHF@F22BGGFFHGH21100BFG1BBF21<E/?E<GHGF11>BGFGHH22222@@F@GF1FF<2@F1F1CG<<1?1A@C@<1111=1>FGFDD@CFGF0GDGGGHCHGHGH000000C;<CCGGE.C0C0C-;?EA.;9B009;000;0999;/;BBF/;BBBFFE-9A99-AB-9A@@@F9;-B9B@M01520:130:000000000-ALUEB:1:1109:19301:28793 1:N:0:0TTCTTTTTTTTCTCTCTTTTTTCTTATTTACTTTTCTTTCTTTTTTCTTCTTCTTTTTTTTTTTTCTTTTTTTTTTTCTCCTTTCTTTTCTTTTTTTTTTCTTTTTTTTCTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTCTCTTTTTTTTTCTTATTTTTCTTTTTTTTTTCCTTTTTCTTTTTCTTCTTTCTTTTTCTTTTTTTTCTCTTTTTTTCTTTTTCTTTTTTTTCTTTC+>11113@BA10B13B1BFG1DAABD2ADA22DBFDAAE12211D1/0AA22D221A11/>>///>01@111>////>01211<>>F111211111//->-<0=000-;-/;:0:0:-/;00009-9--------@--------9;-////;/9///9/://--99//B///;/;/99///9----////;9B////9///:/9///;//////;://9--/;9B9/9/-9//////////9/--9/////
[sejj036@login09 761977.1.]$ tail bioc3101_2016_read2.fastq+AA1>AFCAFFFFGGB1AECGGGG000EGHFH1AEEE/A2A11BAFCFF10A///EEEGGHHGGG>0EE>/EGH@GDC0B1GGFHGGGGGFGFHHFHDEGGECGGF0BB01GHHG0A/</FHGHHBG0</A<CH0FGB<<--<=GH0GH000/AC--:;0CCCB9CFB9CF0009.9.9CFGBF?.9/;9CBBF/9/--9;-AAFB;9--;-A-BFBB-A@---;///99BB-----;;@9----;A9-9-@M01520:130:000000000-ALUEB:1:1109:18959:28767 3:N:0:0CCTGTTTGCTCCCCTCGCTTTCGTGCCTCAGCGTCAATAACTGTCCAGTAAGCTGCCTTCGCAACTGGTGTTCCTCCTCATATCTAAGCATTTCACCGCTACACCTCACATTCCGCTTTCCTCTTCAGTACTCAAGACAAACAGTCTCAATCGCAGTTCCCCAGTTAAGCTCGTAGCTTTCACCTCTGACTTACCTACCCTCCTACGCACCCTTTCCGCCCACTCATTCCGAACACCGCTTGCGACCTCT+AA1>AF@B@DFFGG1AEFAGGGA000FHFGG1BBEEAA2111A2A1DB212111BBFGGHHG///0B0B0FEH22100>BFBFGGG1BFFGGFHFG1EEGEE1>0001/1BFGF0>///BFFHF11212B1BG1BG10B00/B@G1@F2211/<//<?1?100CCD11?F11<<.>.11=<1=<0<000=0D00/00<<C0:CG00;-.;:B99FB0..;@9.://090;/..---;-9--;------:/@M01520:130:000000000-ALUEB:1:1109:19301:28793 3:N:0:0CCTTTTTTCTCCCCTCTCTTTCTTTCCTCTTCTTCTTTTTTTTTCCTTTTTTCCTCCTTCTCCTCTTTTTTTCCTCCTTTTCTCTTCTCTTTTCTCTTCTTCTCCTTTCTTTCCTCTCTCCTCTCCCTTCCTCTTTCTTTTCTTTTTCCTCTTCTTTTTCCTTTTTTTTCTTTTTTTTTTCTCTTTTTTCTTTTCTTTCCTCCTTCTTTCTCTTTTCTCCCTTTTTTTTTTTTTTTTTCTTTCTTCCTTT+111>>BD>>B31111110ABE3A3331D11112A221B11//AA0112211/01AA0BBB1111011@11>>01B10012B2B2B21@@2BBE21222>B12211BB212B>21>1B0<10<0B0000B00B1@1122111211>1<01>111111<?101111<<---/00000--::?/0099000-/990;000000;0/:00009////9://////-/9//-9----------/////////9//
[sejj036@login09 761977.1.]$ tail bioc3101_2016_barcodes.fastq+>111111>1111@M01520:130:000000000-ALUEB:1:1109:18959:28767 2:N:0:0TACTGTACTGAT+>1>1>33B3333@M01520:130:000000000-ALUEB:1:1109:19301:28793 2:N:0:0TTTTTTTCTTTT+>1111111B331
count_seqs.py -i bioc3101_2016_barcodes.fastq,bioc3101_2016_read1.fastq,bioc3101_2016_read2.fastq16807541 : bioc3101_2016_barcodes.fastq (Sequence lengths (mean +/- std): 12.0000 +/- 0.0000)16807541 : bioc3101_2016_read1.fastq (Sequence lengths (mean +/- std): 250.0000 +/- 0.0000)16807541 : bioc3101_2016_read2.fastq (Sequence lengths (mean +/- std): 250.0000 +/- 0.0000)50422623 : Total
grep -n M01520:130:000000000-ALUEB:1:2106:16170:1516 bioc3101_2016_barcodes.fastq629:@M01520:130:000000000-ALUEB:1:2106:16170:1516 2:N:0:0
[sejj036@login09 761977.1.]$ sed '633q;d' bioc3101_2016_read1.fastq@M01520:130:000000000-ALUEB:1:2106:15692:1516 1:N:0:0[sejj036@login09 761977.1.]$ sed '633q;d' bioc3101_2016_read2.fastq@M01520:130:000000000-ALUEB:1:2106:15692:1516 3:N:0:0[sejj036@login09 761977.1.]$ sed '633q;d' bioc3101_2016_barcodes.fastq@M01520:130:000000000-ALUEB:1:2106:15692:1516 2:N:0:0
echo "tail -n 10 $TMPDIR/bioc3101_2016_read1.fastq"
echo "tail -n 10 $TMPDIR/bioc3101_2016_read2.fastq"
Hi Jamie,
tail -n 10 bioc3101_2016_read1.fastq
+AAAA1DC?F11B1BGFGGGCCAGEEG?E1B1DDFGHGGGGHH1E/AAEAEGG1EE?/>//>EFHHH2B11>FFFGHFH1>/</?FGDBGH210?>?CCHHH/<//??2FGHF11<?/AF?CFFDD111F<F.EG./...=CC@:C0C00;FGGFBF@GCAEFGFGGGGFGGGGGG90B/ABBFF-BB-9@@FF@@9@FE9@@-9-AEFFFF/;//9/BFBF/BFFBB?--:A---AB--9--;-;?A99A@M01520:130:000000000-ALUEB:1:1109:18959:28767 1:N:0:0TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGCGCGTAGGCGGCTAGGTAAGTCAGTGGTGAAATCTCCCAGCTTAACTCGGAAACTGCCATTGATACTATTTATCTTGAATACTTTGGAGGTAAGCGGAATTTGTCATGTAGCGGTGAAATGCTTAGATATGACATAGAACACCAATTGCGAAGGCAGCTTACTATACAGTTATTGACACTGAGGCACGAAAGCGTGGGGATCAAAC+AAAA11BAC11B3B1FFGG1AAFEEG0E1D2DFFGGEGGGHH1B//AAAEEF1EG///00BBFGHF@F22BGGFFHGH21100BFG1BBF21<E/?E<GHGF11>BGFGHH22222@@F@GF1FF<2@F1F1CG<<1?1A@C@<1111=1>FGFDD@CFGF0GDGGGHCHGHGH000000C;<CCGGE.C0C0C-;?EA.;9B009;000;0999;/;BBF/;BBBFFE-9A99-AB-9A@@@F9;-B9B@M01520:130:000000000-ALUEB:1:1109:19301:28793 1:N:0:0TTCTTTTTTTTCTCTCTTTTTTCTTATTTACTTTTCTTTCTTTTTTCTTCTTCTTTTTTTTTTTTCTTTTTTTTTTTCTCCTTTCTTTTCTTTTTTTTTTCTTTTTTTTCTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTCTCTTTTTTTTTCTTATTTTTCTTTTTTTTTTCCTTTTTCTTTTTCTTCTTTCTTTTTCTTTTTTTTCTCTTTTTTTCTTTTTCTTTTTTTTCTTTC+>11113@BA10B13B1BFG1DAABD2ADA22DBFDAAE12211D1/0AA22D221A11/>>///>01@111>////>01211<>>F111211111//->-<0=000-;-/;:0:0:-/;00009-9--------@--------9;-////;/9///9/://--99//B///;/;/99///9----////;9B////9///:/9///;//////;://9--/;9B9/9/-9//////////9/--9/////
[sejj036@login06 761977.1.]$ tail -n 10 bioc3101_2016_read2.fastq
+AA1>AFCAFFFFGGB1AECGGGG000EGHFH1AEEE/A2A11BAFCFF10A///EEEGGHHGGG>0EE>/EGH@GDC0B1GGFHGGGGGFGFHHFHDEGGECGGF0BB01GHHG0A/</FHGHHBG0</A<CH0FGB<<--<=GH0GH000/AC--:;0CCCB9CFB9CF0009.9.9CFGBF?.9/;9CBBF/9/--9;-AAFB;9--;-A-BFBB-A@---;///99BB-----;;@9----;A9-9-@M01520:130:000000000-ALUEB:1:1109:18959:28767 3:N:0:0CCTGTTTGCTCCCCTCGCTTTCGTGCCTCAGCGTCAATAACTGTCCAGTAAGCTGCCTTCGCAACTGGTGTTCCTCCTCATATCTAAGCATTTCACCGCTACACCTCACATTCCGCTTTCCTCTTCAGTACTCAAGACAAACAGTCTCAATCGCAGTTCCCCAGTTAAGCTCGTAGCTTTCACCTCTGACTTACCTACCCTCCTACGCACCCTTTCCGCCCACTCATTCCGAACACCGCTTGCGACCTCT+AA1>AF@B@DFFGG1AEFAGGGA000FHFGG1BBEEAA2111A2A1DB212111BBFGGHHG///0B0B0FEH22100>BFBFGGG1BFFGGFHFG1EEGEE1>0001/1BFGF0>///BFFHF11212B1BG1BG10B00/B@G1@F2211/<//<?1?100CCD11?F11<<.>.11=<1=<0<000=0D00/00<<C0:CG00;-.;:B99FB0..;@9.://090;/..---;-9--;------:/@M01520:130:000000000-ALUEB:1:1109:19301:28793 3:N:0:0CCTTTTTTCTCCCCTCTCTTTCTTTCCTCTTCTTCTTTTTTTTTCCTTTTTTCCTCCTTCTCCTCTTTTTTTCCTCCTTTTCTCTTCTCTTTTCTCTTCTTCTCCTTTCTTTCCTCTCTCCTCTCCCTTCCTCTTTCTTTTCTTTTTCCTCTTCTTTTTCCTTTTTTTTCTTTTTTTTTTCTCTTTTTTCTTTTCTTTCCTCCTTCTTTCTCTTTTCTCCCTTTTTTTTTTTTTTTTTCTTTCTTCCTTT+111>>BD>>B31111110ABE3A3331D11112A221B11//AA0112211/01AA0BBB1111011@11>>01B10012B2B2B21@@2BBE21222>B12211BB212B>21>1B0<10<0B0000B00B1@1122111211>1<01>111111<?101111<<---/00000--::?/0099000-/990;000000;0/:00009////9://////-/9//-9----------/////////9//
That's what I thought as well, but both the beginning and the end align. That is besides the identifier that specifies which read the sequence is from: 1:N:0:0 vs 3:N:0:0
[sejj036@login06 fastq-join_joined]$ head -n 12 fastqjoin.join.fastq@M01520:130:000000000-ALUEB:1:2106:16170:1516 1:N:0:0TACAGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTAGGCGGCCTTCTAAGTCGAACGTGAAATCCCTGGGCTCAACCCGGGAACTGCGTCCGATACTGGAAGGCTTGAATCCGGGAGAGGGATGCGGAATTCCAGGTGTAGCGGTGAAATGCGTAGATATCTGGAGGAACACCGGTGGCGAAGGCGGCATCCTGGACCGGTATTGACGCTGAGGAGCGAAAGCCAGGGGAGCAAACGGG+BBBABF;AADEDFFGGGGGG;GHGGGFFHHGHHFFHGGG+BFFCHEEEEEG?D9E>@6G?FBEFHHG?FGEHHEEHGH7F$B?EFG2FCF:;$?F<CFGHHDA@>DFC>CHFFG>>>GHHGE<60ECGGHHGGCCCFE<BGFBCFFGHHFFFEGEBHFGHHFEEFFFFGGFHHGBFGGHHGEE@EGEEFFFFFHE:EEEFGB:EBGFFFGFFC1;D;FFEE9FDFGEF$ACGGGGGEG8GGFFFFAFF4A>A>@M01520:130:000000000-ALUEB:1:2106:16740:1519 1:N:0:0TACAGGGGGAGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTAGGCGGCCTTCTAAGTCGAACGTGAAATCCCTGGGCTCAACCCAGGAACTGCGTCCGATACTGGAAGGCTTGAATCCGGGAGAGGGATGCGGAATTCCAGGTGTAGCGGTGAAATGCGTAGATATCTGGAGGAACACCGGTGGCGAAGGCGGCATCCTGGACCGGTATTGACGCTGAGGCGCGAAAGCCAGGGGAGCAAACGGG+AAB?5CABB0A5C9GFGGG?99HGEGGGGGH<HFCFG7C,BFGFEA7EEEE?G>(E7G7HBF;GGGGAFEEGHE;FFGGGHC/CFG/CBC00CGEC<<FDDD7</GDC>AHFHFFC><GFFC<=DC0G?HHHGGCDHFGGFBBCFFHGFFFFGEGCEE;GFFBB>:>DGBBFBBBFFHH9DE?9>CEEABEEAHEEEEEAB15A01;AAA;/A19*A9A4911EEEEA000GF;GGGFFGGFFFFAFF3>>>>@M01520:130:000000000-ALUEB:1:2106:14606:1523 1:N:0:0TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTCCTTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGAGTGCAGGAGAGAAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGATATCGAGAGGAACACCAGTGGCGAAGGCGAGTCTCTGGACGACTCCTGACACTGAGGCACGAAAGCCGGGGGAGCAAACAGG+BBBB3BF@FFBCB4AFFFGFG:HGGGGGHHD;HGDHFGC*BF:GGG/GGGFC-FFE7GG7GG4FGHHHFGHHHHFF<:91:?E<>E2EGF2@EHFDHG?4G>FHFF5F>FGEB/D/,>GHHFFHBG@<FGFGB1F<DGHHFBB<C=00<EFHHG>0FBEGGEFGFBEGGF>EFFCC1B@2FEF>B9CFFFEF/HEFEEFGBB;BFFBFFA92A1F(DFGFD1A;1GFA00EGGEEE91FGGFF:FFFFAAAAA
sed 's/ 1:N:0:0//g' bioc3101_2016_read1.fastq > bioc3101_2016_read1.fixed.fastq
split_libraries_fastq.py -i $TMPDIR/fastq-join_joined/fastqjoin.join.fastq -b $TMPDIR/fastq-join_joined/fastqjoin.join_barcodes.fastq -o $TMPDIR/slout -m $TMPDIR/map.tsv --barcode_type 12
Traceback (most recent call last):
File "/imports/home0/sejj036/qiime191/bin/split_libraries_fastq.py", line 365, in <module> main() File "/imports/home0/sejj036/qiime191/bin/split_libraries_fastq.py", line 344, in main for fasta_header, sequence, quality, seq_id in seq_generator: File "/imports/home0/sejj036/qiime191/lib/python2.7/site-packages/qiime/split_libraries_fastq.py", line 317, in process_fastq_single_end_read_file parse_fastq(fastq_read_f, strict=False, phred_offset=phred_offset)): File "/imports/home0/sejj036/qiime191/lib/python2.7/site-packages/skbio/parse/sequences/fastq.py", line 174, in parse_fastq seqid)skbio.parse.sequences._exception.FastqParseError: Failed qual conversion for seq id: M01520:130:000000000-ALUEB:1:2106:16170:1516. This may be because you passed an incorrect value for phred_offset.