I'm new in this field, having some problems with my first analysis.
Could you help me to solve the problems of reference?
I'm doing my works with 16S rRNA sequences.
My problems are,
1. Reference sequences: RDP
- I want to use RDP reference sequence, but I have no idea which RDP data should I use for analysis; training set, unaligned, aligned.
- I want to know the meaning of "RDP Training set". What is RDP Training set? Is this using when the taxonomy assignment method is 'RDP classifyer'?
2. Error in Taxonomy assignment with RDP reference data
I got error when 'assign_taxonomy.py' with unaligned RDP data as reference sequence.
pick_open_reference_otus.py -i seqs_chimera_filtered.fna -o w_rdp_otus/ -r unaligned_rdp.fa -f -a --suppress_taxonomy_assignment
Then I tried to assignment with unaligned rdp reference sequence but I failed.
Here is the command:
assign_taxonomy.py -i rep_set.fna -r Trimmed_reads/unaligned_rdp.fa -t Trimmed_reads/trainset16_022016.rdp/trainset16_022016.rdp.tax -o unaligned_rdp_assignment
Traceback (most recent call last):
File "/qiime/qiime/build/scripts-2.7/assign_taxonomy.py", line 417, in <module>
File "/qiime/qiime/build/scripts-2.7/assign_taxonomy.py", line 394, in main
File "/qiime/python/python2/lib/python2.7/site-packages/qiime-1.9.1.dev0-py2.7.egg/qiime/assign_taxonomy.py", line 1306, in __call__
result = self._uc_to_assignments(app_result['ClusterFile'])
File "/qiime/python/python2/lib/python2.7/site-packages/qiime-1.9.1.dev0-py2.7.egg/qiime/assign_taxonomy.py", line 1364, in _uc_to_assignments
tax = self.id_to_taxonomy[subject_id].split(';')
But, when I use greengenes as reference, there's no error. What should I do for solve this problem?
I need some help to understand how to use the right reference sequence in each process.
2017. 07. 06. THU.
- Hyojung Kim