Hi, I'm a new QIIME user. I have one 454 metadate file (16S rRNA). It has 7 barcodes, because I have 7 different samples. I've splitted the seqs in 7 fasta files (by Bioedit program). Now, I want to create and validate the map file in QIIME.
I have doubts about how I must create the map file. I've done a map file with sequences related to one barcode and I tried to execute but I couldn't. I send you a screenshot of the error message. I don't know if the message is for the script or about the map file.
When I have different barcodes, I have to create one map file each one? And each map file must have all sequences assigned to that barcode?
For more information, I've installed QIIME Virtual Box.
I hope that someone understand me.