reads.fastq and barcodes.fastq
3- Next, I'm to use split_libraries_fastq
I use barcodes.fastq from step 2, and fastqjoin.join.fastq from step 1.
split_libraries_fastq.py -i fastqjoin.join.fastq -m qiime_mapping.txt -q 19 -o slout -b barcodes.fastq --barcode_type 12
Unfortunately, I don't get any sequences.
My run ends with an exception raised for
"Mean of empty slice"
I've following queries to make sure I'm not barking at the wrong tree:
1) Am I using correct set of file at step 3?
2) In my mapping file, what barcodes should I be using?
a) plainly concatenate forward and reverse? (without any rev com of reverse barcode)
I got this information from this
b) Or, concatenate forward primer and reverse the barcode for reverse primer?
c) Something I do not know of.
In my mapping file I've column for forward and reverse primer.
Attached mapping file for reference.
Am I doing anything wrong in it? I validated it using validate script from qiime. No errors were found.
Thank you very much for your patience.