I'm stuck in the 454 sequence analysis of 7 different environments. The objective is to have the OTUs of the 7 samples separately and then compare.
I have an initial fasta file (where the 454 sequences of the 7 environments are mixed). I already ran the mapping file and split_library validation. From this second script, I got the seqs.fna file. And it is from there where I suppose that I must divide the samples corresponding to their origins. But I do not know how to do it.
In turn, I also followed the instructions of the tutorial, so the next step was pick_de_novo_otus.py. But the otus he generated is a mixture of all environments.
I hope you can help me again