My mapping file looks like this:
#SampleID BarcodeSequence LinkerPrimerSequence Description
101-P-MS515F AAAACAAA GTGCCAGCMGCCGCGGTAA 101-P-MS515F
29-MS515F AAAACAAC GTGCCAGCMGCCGCGGTAA 29-MS515F
44-MS515F AAAACAAG GTGCCAGCMGCCGCGGTAA 44-MS515F
MER-MS515F AAAACAAT GTGCCAGCMGCCGCGGTAA MER-MS515F
WER-MS515F AAAACACA GTGCCAGCMGCCGCGGTAA WER-MS515F
Here are some random excerpts from my .fna file before split libraries
>101-P-MS515F::M02696:21:000000000-AFKTF:1:2114:28714:18709
AAAACAAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGAGCAGGCGGTCTCATAAGTCTGATGTGAAAGCCCACGGCTCAACCGAGGAAGGTCATTGGAAACTGGGGGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCGCGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG
>29-MS515F::M02696:21:000000000-AFKTF:1:2107:16203:25258
AAAACAACTACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGG
>WER-MS515F::M02696:21:000000000-AFKTF:1:2102:20315:17355
AAAACACATACGGAGGGGGCGAGCGTTGTCCGAGGTTACTGGGCGTAAAGGGCGCGTAGACGGGGTGGCAAGTCCGCTGTGAAAGCCCGGCGCTTAACGCCGGAGGGGCGGTGGATACTGCCAGTCTTGAAGGTGCTAGGGACAGATGGAATTACCAGTGTAGCGGTGAAATGCGTAGATATTGGTAGGAACACCAGTGGCGAAGGCGGTCTGTTGGAGCACTCATGACGCTGAGGCGCGAAAGCTGGGGGAGCGAACGGG
And here are some random excepts from my .qual file before running split libraries
>101-P-MS515F::M02696:21:000000000-AFKTF:1:2114:28714:18709
40 40 40 40 40 40 40 40 37 37 33 33 36 36 38 38 38 33 32 33 37 37 39 37 39 39 39 39 39 39 39 38 34 36 39 38 36 35 35 37 38 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 34 34 40 34 40 40 40 40 30 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 29 40 40 40 18 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 28 14 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 31 40 31 40 31 40 40 40 40 40 40 40 27 27 40 27 27 28 40 40 40 40 40 40 40 40 40 40 40 40 40 40 34 28 26 40 15 40 40 40 40 40 25 40 40 40 40 40 27 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 25 31 40 28 30 40 40 40 40 40 40 27 40 14 40 40 37 40 40 40 40 40 40 38 40 40 40 38 40 20 40 38 37 40 40 40 40 40 20 37 15 38 38 38 39 37 37 17
>WER-MS515F::M02696:21:000000000-AFKTF:1:2107:20652:25386
40 40 40 40 40 40 40 40 37 32 35 33 32 37 36 37 38 37 20 33 37 33 38 39 39 39 39 39 39 37 37 38 17 32 36 35 38 18 37 39 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 38 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 37 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 34 40 38 40 40 40 40 40 38 40 40 40 34 40 40 38 40 40 38 35 32 40 38 40 39 40 40 40 38 40 40 40 40 36 39 39 39 38 37 37 40 40 40 40 40 39 37 38 38 38 38 37 37 37 38
>44-MS515F::M02696:21:000000000-AFKTF:1:1106:23550:16882
40 40 40 40 40 40 40 40 37 37 33 33 33 38 38 37 38 37 33 37 38 38 38 38 38 38 38 38 38 39 32 36 37 39 38 38 38 39 39 39 39 39 38 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 40 40 40 39 40 40 40 40 40 40 39 40 40 17 40 39 40 38 40 38 38 40 38 40 40 40 40 39 40 40 37 39 40 39 40 40 40 39 40 39 40 40 38 40 38 39 39 39 39 39 39 39 39 39 39 39
Does anyone have a solution for this problem? Any help will be much appreciated.
Cheers,
Sam
AssertionError: Non-header line passed as input. Header must start with '@'.
Will this work on this file?
I did manage to run this through the demultiplex_fasta.py script, which allowed me to pick otu's which couldn't before it. However, I realise split libraries does some additional quality filtering thought it might be a good idea to run it though that instead.
Any ideas?
Thanks again,
Sam