How t remove three primers using split_library.py

[monica steffi matchado]

Dear All,

I have pair-end Illumina raw reads for V1-V9 region. During sequencing, 3 primers were used as follows:

    V13F    AGAGTTTGATGMTGGCTCAG
    V13R    TTACCGCGGCMGCSGGCAC
    V46F    GTGCCAGCTGCCGCGGTAATAC
    V46R    CTGACGACAGCCATGCA
    V79F    GCAACGAGCGCAACCCC
    V79R    AAGGAGGTGATCCAGGC

How do I incorporate these primers in map.txt file and remove using split_library at once?

[TonyWalters]

Hello Monica,

For the LinkerPrimerSequence and ReversePrimer fields, put in all primer variants, comma separated, with no spaces, e.g. 

AGAGTTTGATGMTGGCTCAG,GTGCCAGCTGCCGCGGTAATAC,GCAACGAGCGCAACCCC

for the LinkerPrimerSequence fields.