About greengene database

25 views
Skip to first unread message

Jia Zhou

unread,
Nov 23, 2016, 1:21:26 AM11/23/16
to Qiime 1 Forum
Hi, Sir

In DeSantis TZ. et al, 2006, greengenes is chimera-checked, does it mean that we do not need to do chimeric sequences filtering when using qiime to analysis?

Thanks

Jia

zech xu

unread,
Nov 28, 2016, 12:57:55 PM11/28/16
to Qiime 1 Forum
Hi Jia,

It means the sequences in greengene are cleaned from chimeras, but you may have chimera in your reads (primarily from PCR). This is usually the problem people worry when coming to chimeras.
Reply all
Reply to author
Forward
0 new messages