assign_taxonomy.py -i FILE.fasta -r 97_otus.fasta -t 97_otu_taxonomy.txt
That generates the following files:
FILE_tax_assignments.log
FILE_tax_assignments.txt
Now, I would like to generate the final.biom but, using the following command:
biom add-metadata -i FILE_tax_assignments.txt -o final.biom -m FILE_Map.txt
I'm receiving an error:
ValueError: could not convert string to float: Unassigned
I converted the file in biom as well but with the same kind of error. Can somebody explain me better what's the error in the command please?
Thanks in advance!
$ biom add-metadata -i ~/merged.biom -o ~/final.biom --observation-metadata-fp ~/FILE_tax_assignments.txt --observation-header OTUID,taxonomy --sc-pipe-separated
This generated the file final.biom. I am trying to understand if the file generated is correct and if I can proceed with it for the creation of the graphs and downstream analysis.
Thanks in advance again